LC-MS/MS based quantification of steroidal biomarkers in polycystic ovary syndrome induced rats

Agrawal, Sristi; Bisen, Amol Chhatrapati; Sanap, Sachin Nashik; Biswas, Arpon; Kumar, Mukesh; Verma, Sagar; Bhatta, Rabi Sankar

doi:10.1016/j.jpba.2023.115484

Cited by 7 publications

(2 citation statements)

References 59 publications

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“…This approach holds promise for ocular therapeutic protein delivery with minimal systemic exposure and toxicity. [ 226–229 ] The utilization of receptor/ligand interactions offers a significant advantage in this pursuit. By augmenting the permeability of membranes for various biopharmaceuticals and macromolecules, it facilitates intracellular delivery, primarily through receptor‐ and ligand‐mediated endocytosis.…”

Section: Novel Polymeric Conjugatesmentioning

confidence: 99%

Polymers and their engineered analogues for ocular drug delivery: Enhancing therapeutic precision

Biswas,

Kumar,

Choudhury

et al. 2024

Biopolymers

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Ocular drug delivery is constrained by anatomical and physiological barriers, necessitating innovative solutions for effective therapy. Natural polymers like hyaluronic acid, chitosan, and gelatin, alongside synthetic counterparts such as PLGA and PEG, have gained prominence for their biocompatibility and controlled release profiles. Recent strides in polymer conjugation strategies have enabled targeted delivery through ligand integration, facilitating tissue specificity and cellular uptake. This versatility accommodates combined drug delivery, addressing diverse anterior (e.g., glaucoma, dry eye) and posterior segment (e.g., macular degeneration, diabetic retinopathy) afflictions. The review encompasses an in‐depth exploration of each natural and synthetic polymer, detailing their individual advantages and disadvantages for ocular drug delivery. By transcending ocular barriers and refining therapeutic precision, these innovations promise to reshape the management of anterior and posterior segment eye diseases.

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Section: Novel Polymeric Conjugatesmentioning

confidence: 99%

Polymers and their engineered analogues for ocular drug delivery: Enhancing therapeutic precision

Biswas,

Kumar,

Choudhury

et al. 2024

Biopolymers

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“…The psychological impact on patients has been profound, with increased anxiety, depression, isolation, grief, and disrupted social support networks. Moreover, there is a substantial need for healthcare providers and policymakers to recognize the potential risks faced by females such as polycystic ovary syndrome patients and to ensure their adequate protection and support during the pandemic [ 100 , 101 , 102 ]. Other diseases like mucormycosis and keratomycosis fungal infection that has gained attention during the COVID‐19 pandemic.…”

Section: Navigating the Pandemic: Impacts And Interventionsmentioning

confidence: 99%

COVID‐19 retreats and world recovers: A silver lining in the dark cloud

Bisen

Agrawal

Sanap

et al. 2023

Health Care Science

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The coronavirus disease (COVID‐19), which the World Health Organization classified as the Sixth Public Health Emergency Of International Concern (PHEIC) on January 30, 2020, is no longer a PHEIC. Millions were affected due to unawareness. The increase in fatalities and shortage of medicine was the first outrage of COVID‐19. As per the Johns Hopkins COVID‐19 resource center database, it was observed that the disease has spread dynamically across 200+ nations worldwide affecting more than 600 million people from 2019 to 2023, and over thousands of people were victimized regularly at a 2% mortality rate (approx.). In the midway, the mutant variants of concern like omicron, and delta have also created havoc and caused significant impact on public health, global economy, and lifestyle. Since 2019, 3 years now passed and the dynamic disease statistics seem decelerated; moreover, the prevalence of COVID‐19 is also fading. The Johns Hopkins resource center has also stopped recording the data of the global pandemic recently from March 10, 2023. Hence, based on the facts, we are presenting a concise report on the pandemic from 2019 to 2023, which includes a brief discussion of the global pandemic. We have highlighted global epidemiology, emphasizing the Indian COVID scenario, vaccination across the globe, and the psychosocial and geopolitical consequences of COVID‐19 with a brief background to pathology, clinical management, and the worldwide response against triage. A lot has changed and still needs to change after three tough years of COVID‐19. Even though science has progressed and advanced research in medicine is pointing toward future generations, there is no standard care supplied for COVID‐19‐like calamities. COVID‐19 cases might have declined but its influence on the society is still stagnant. This COVID experience has taught us that, despite our bleak beginnings, there is always hope for the future and that we must act with foresight to improve things for future generations.

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Codetermination of antimicrobial agents in rabbit tear fluid using LC–MS/MS assay: Insights into ocular pharmacokinetic study

Bisen,

Mishra,

Agrawal

et al. 2024

J Mass Spectrom

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Managing ocular microbial infections typically requires pharmacotherapy using antibiotic eye drops, such as moxifloxacin hydrochloride (MFX), combined with an antifungal agent like amphotericin B (AB). We carried out and validated an LC–MS/MS assay to quantify these compounds in rabbit tear fluid in order to look into the pharmacokinetics of these two drugs. We employed a protein precipitation technique for the extraction of drugs under examination. A Waters Symmetry C18 column was used to separate the analytes and internal standard. The composition of the mobile phase was like (A) 0.1% v/v formic acid in water and (B) methanol. The detection of MFX and AB was accomplished through the utilization of positive ion electrospray ionization under multiple reaction monitoring mode. The linearity curves for both analytes exhibited an acceptable trendline across a concentration range of 2.34–300 ng/mL for MFX and 7.81–1000 ng/mL for AB in surrogate rabbit tear fluid. The lower limit of quantitation for MFX was 2.34 ng/mL, while for AB, it was 7.81 ng/mL. The approach was strictly validated, encompassing tests of selectivity, linearity (with r2 > 0.99), precision, accuracy, matrix effects, and stability. Consequently, we employed this method to evaluate the pharmacokinetics profiles of MFX and AB in rabbit tear fluid following single topical doses.

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LC-MS/MS based quantification of steroidal biomarkers in polycystic ovary syndrome induced rats

Cited by 7 publications

References 59 publications

Polymers and their engineered analogues for ocular drug delivery: Enhancing therapeutic precision

Polymers and their engineered analogues for ocular drug delivery: Enhancing therapeutic precision

COVID‐19 retreats and world recovers: A silver lining in the dark cloud

Codetermination of antimicrobial agents in rabbit tear fluid using LC–MS/MS assay: Insights into ocular pharmacokinetic study

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