LC–MS method for the estimation of Δ8-THC and 11-nor-Δ8-THC-9-COOH in plasma

Hammell, Dana C.; Earles, D. Caroline; Stinchcomb, Audra L.

doi:10.1016/j.jpba.2004.11.055

Cited by 15 publications

(5 citation statements)

References 12 publications

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“…In contrast to GC, no decomposition of the cannabinoids occurs during liquid chromatography and hence the cannabinoid acid forms may be analyzed directly. Several papers report the application of LC-MS(/MS) [8][9][10][11][12][13][14]. Most of them require high sample volume (1 mL) to achieve high sensitivity, they are focused on just one compound and/or the method is not fully validated (following all criteria for chromatographic assays).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of THC and its metabolites in blood using liquid chromatography–tandem mass spectrometry

Fernández

Boeck

Wood

et al. 2008

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of THC and its metabolites in blood using liquid chromatography–tandem mass spectrometry

Fernández

Boeck

Wood

et al. 2008

Journal of Chromatography B

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“…Pharmacokinetic studies were conducted using the LC-MS method for the determination of Δ 8 -THC and its metabolite Δ 8 -THC-COOH in guinea pig plasma. A liquid–liquid extraction protocol was developed to extract the target analytes from guinea pig plasma using acetonitrile: ethyl acetate (50:50, v / v ) [ 75 ].…”

Section: Analysis Of δ 8 -Thcmentioning

confidence: 99%

Chemistry and Pharmacology of Delta-8-Tetrahydrocannabinol

Abdel-Kader,

Radwan,

Metwaly

et al. 2024

Molecules

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Cannabis sativa is one of the oldest plants utilized by humans for both economic and medical purposes. Although the use of cannabis started millennia ago in the Eastern hemisphere, its use has moved and flourished in the Western nations in more recent centuries. C. sativa is the source of psychoactive cannabinoids that are consumed as recreational drugs worldwide. The C21 aromatic hydrocarbons are restricted in their natural occurrence to cannabis (with a few exceptions). Delta-9-tetrahydrocannabinol (Δ9-THC) is the main psychoactive component in cannabis, with many pharmacological effects and various approved medical applications. However, a wide range of side effects are associated with the use of Δ9-THC, limiting its medical use. In 1966, another psychoactive cannabinoid, Delta-8-tetrahydrocannabinol (Δ8-THC) was isolated from marijuana grown in Maryland but in very low yield. Δ8-THC is gaining increased popularity due to its better stability and easier synthetic manufacturing procedures compared to Δ9-THC. The passing of the U.S. Farm Bill in 2018 led to an increase in the sale of Δ8-THC in the United States. The marketed products contain Δ8-THC from synthetic sources. In this review, methods of extraction, purification, and structure elucidation of Δ8-THC will be presented. The issue of whether Δ8-THC is a natural compound or an artifact will be discussed, and the different strategies for its chemical synthesis will be presented. Δ8-THC of synthetic origin is expected to contain some impurities due to residual amounts of starting materials and reagents, as well as side products of the reactions. The various methods of analysis and detection of impurities present in the marketed products will be discussed. The pharmacological effects of Δ8-THC, including its interaction with CB1 and CB2 cannabinoid receptors in comparison with Δ9-THC, will be reviewed.

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“…Pharmacokinetic studies were conducted using the LC-MS method for the determination of ∆ 8 -THC and its metabolite ∆ 8 -THC-COOH in guinea pig plasma. A liquid-liquid extraction protocol was developed to extract the target analytes from guinea pig plasma using acetonitrile: ethyl acetate (50:50, v/v) [75].…”

Section: Analysis Of δ 8 -Thc and δ 9 -Thc Metabolites In Different B...mentioning

confidence: 99%

Chemistry and Pharmacology of Delta-8-Tetrahydrocannabinol

Abdel-Kader,

Radwan,

Metwaly

et al. 2024

Preprint

View full text Add to dashboard Cite

Cannabis sativa is one of the oldest plants utilized by humans for both economic and medical purposes. Although the use of cannabis started millennia ago in the Eastern part of the hemisphere, its use has moved and flourished in the Western Nations in more recent centuries. C. sativa is the source of psychoactive cannabinoids that are consumed as recreational drugs worldwide. The C21 aromatic hydrocarbons are restricted in their natural occurrence to cannabis (with a few exceptions). Delta-9-tetrahydrocannabinol (Δ9-THC) is the main psychoactive component in cannabis with many pharmacological effects and various approved medical applications. However, a wide range of side effects are associated with the use of Δ9-THC, limiting its medical use. In 1966, another psychoactive cannabinoid, Delta-8-tetrahydrocannabinol (Δ8-THC) was isolated from marijuana grown in Maryland but in very low yield. Δ8-THC is gaining increased popularity due to its better stability and easier synthetic manufacturing procedures compared to Δ9-THC. Passing the U.S. Farm Bill in 2018 led to an increase in the sale of Δ8-THC in the United States. The marketed products contain Δ8-THC from synthetic sources. In this review, methods of extraction, purification, and structure elucidation of Δ8-THC will be presented. The issue of whether Δ8-THC is a natural compound or an artifact will be discussed, and the different strategies for its chemical synthesis will be presented. Δ8-THC of synthetic origin is expected to contain some impurities due to residual amounts of starting materials and reagents as well as side products of the reactions. The various methods of analysis and detection of impurities present in the marketed products will be discussed. The pharmacological effects of Δ8-THC, including its interaction with CB1 and CB2 cannabinoid receptors in comparison with Δ9-THC, will be reviewed.

show abstract

LC–MS method for the estimation of Δ8-THC and 11-nor-Δ8-THC-9-COOH in plasma

Cited by 15 publications

References 12 publications

Simultaneous analysis of THC and its metabolites in blood using liquid chromatography–tandem mass spectrometry

Simultaneous analysis of THC and its metabolites in blood using liquid chromatography–tandem mass spectrometry

Chemistry and Pharmacology of Delta-8-Tetrahydrocannabinol

Chemistry and Pharmacology of Delta-8-Tetrahydrocannabinol

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