LC-MS Data Analysis for Differential Protein Expression Detection

Abstract

In proteomic studies, liquid chromatography coupled with mass spectrometry (LC-MS) is a common platform to compare the abundance of various peptides that characterize particular proteins in biological samples. Each LC-MS run generates data consisting of thousands of peak intensities for peptides represented by retention time (RT) and mass-to-charge ratio (m/z) values. In label-free differential protein expression studies, multiple LC-MS runs are compared to identify differentially abundant peptides between distinct biological groups. This approach presents a computational challenge because of the following reasons (i) substantial variation in RT across multiple runs due to the LC instrument conditions and the variable complexity of peptide mixtures, (ii) variation in m/z values due to occasional drift in the calibration of the mass spectrometry instrument, and (iii) variation in peak intensities caused by various factors including noise and variability in sample handling and processing. In this chapter, we present computational methods for quantification and comparison of peptides by label-free LC-MS analysis. We discuss data preprocessing methods for alignment and normalization of LC-MS data. Also, we present multivariate statistical methods and pattern recognition methods for detection of differential protein expression from preprocessed LC-MS data.