LC-MS-based metabolomics

Zhou, Bin; Xiao, Jun; Tuli, Leepika; Ressom, Habtom W.

doi:10.1039/c1mb05350g

Cited by 466 publications

(393 citation statements)

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“…Targeted approach focuses on identifying and quantifying selected metabolites such as substrate of an enzyme, direct products of a protein, a particular class of a compound or members of a particular pathway. While targeted approach is usually hypothesis driven, untargeted metabolites can generate new hypothesis for further tests by measuring ideally all the metabolites of a biological system [2] . The common analytical tools applied to metabolomics are nuclear magnetic resonance (NMR), gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-mass spectrometry (LC/MS).…”

Section: Oral Leukoplakia and Squamous Cell Carcinomamentioning

confidence: 99%

“…These building blocks and the biochemical intermediates generated during their production and utilization are collectively referred to as metabolites [1] . Metabolomics is the comprehensive analysis of all metabolites in a biological system [2] . Currently with the advent of sophisticated technologies, metabolomics has become a powerful tool to gain insight into various cellular functions.…”

Section: Introductionmentioning

confidence: 99%

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Salivary Metabolomics and Oral Carcinogenesis

Sridharan

Patankar

2016

Journal of Tumor

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provides an overview of the use of salivary metabolomics in OSCC and presents the current literature findings and future implications. INTRODUCTIONMetabolism is a set of processes catalyzing the production of energy and cellular building blocks by the cell utilizing the nutrients obtained from the environment. These building blocks and the biochemical intermediates generated during their production and utilization are collectively referred to as metabolites [1] . Metabolomics is the comprehensive analysis of all metabolites in a biological system [2] . Currently with the advent of sophisticated technologies, metabolomics has become a powerful tool to gain insight into various cellular functions. It enables the assessment of broad range of endogenous and exogenous metabolites which have an important role in biological systems thus serving as an attractive candidate to understand disease phenomenon. The human metabolome is composed of diverse group of low molecular weight structures like lipids, aminoacids, peptides, nucleic acid and organic acids, vitamins, thiols and carbohydrates [3] . Salivary metabolomics is an emerging field for identification of disease related biomarkers which may be helpful in early diagnosis as well as monitoring of oral cancer. The presence of wide range of biological components in human saliva, its close proximity to the lesion, the non-invasive nature of collection and decreased probability of spread of infection makes it an ideal bodily fluid for biomarker identification. Commonly employed analytical techniques for metabolomics include nuclear magnetic resonance (NMR) spectroscopy, Liquid chromatography/ mass spectrometry techniques, gas chromatography/ mass spectrometry among others. ABSTRACTCancers of the oral cavity has received reasonable importance especially in developing countries due to its significant morbidity and mortality rates. Oral squamous cell carcinoma is the common type of cancer occurring either de novo or from pre-existing potentially malignant disorders like leukoplakia. Ever since the disease has been identified, efforts are made to understand its pathogenesis in order to identify methods for early diagnosis, evolve appropriate treatment strategies and improve prognosis. Though tobacco, alcohol and microbes are considered important risk factors for OSCC; these are usually accompanied by various genetic and epigenetic factors. Modern analytical technologies provide comprehensive methods for identification of these molecular changes which can serve as an important biomarker. Metabolomics forms a part of 'omic' group that also includes genomics, proteomics and transcriptomics which is associated with the identification and quantitation of small molecules involved in metabolic reactions. The identification of a wide range of metabolites in bodily fluids is considered as an important diagnostic tool for assessment of disease biomarkers. The role of saliva as an important diagnostic tool for biomarker analysis has been accepted as a safe alternative to blood owing t...

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Section: Oral Leukoplakia and Squamous Cell Carcinomamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Salivary Metabolomics and Oral Carcinogenesis

Sridharan

Patankar

2016

Journal of Tumor

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“…A técnica de LC-MS utilizando técnicas de ionização brandas, principalmente ESI, tornam MS mais robusta para análises de rotina e, por isso, tem sido extensivamente empregada em metabolômica. [149][150][151][152][153][154] Além disso, a alta resolução e reprodutibilidade de LC-MS estabelece uma excelente base para o posterior processamento e análise de dados multivariados. Por isso, análises baseadas em LC-MS vêm sendo cada vez mais utilizadas no diagnóstico de doenças humanas.…”

Section: Cromatografia Líquida Acoplada à Espectrometria De Massasunclassified

“…Utilizando cromatografia líquida de interação hidrofílica (Hydrophilic Interaction Liquid Chromatography, HILIC) é possível separar compostos polares como açúcares, amino açúcares, vitaminas, ácidos carboxílicos, nucleotídeos, etc. 153 Assim, a combinação de diferentes colunas cromatográficas e modos de ionização permite à técnica de LC-MS cobrir uma vasta gama de metabólitos, justificando seu extenso emprego em metabolômica.…”

Section:  Página 74unclassified

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Avaliação analítica de potenciais biomarcadores para câncer de bexiga em urina

Alberice¹

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A Deus pela dádiva da vida e força para vencer os obstáculos e alcançar meus objetivos.Aos meus pais Claudir e Aparecida pela oportunidade de estudar, por todo apoio e amor incondicional. Ao meu irmão Wellington pelo carinho e paciência.Ao meu namorado Camilo pelo companheirismo, amor, paciência e por me ajudar a ser uma pessoa melhor.Aos meus familiares que contribuíram e contribuem para o meu crescimento agradeço pelo apoio, carinho e torcida.Às minhas queridas amigas de infância Aline, Carol, Rachel, Giovana e Vanessa com as quais sei que posso contar independente da distância. Em especial agradeço à Rachel por me escutar e me entender, ainda que nenhuma palavra seja dita.Agradeço imensamente ao Prof. Dr. Emanuel, por todos esses anos de orientação e amizade, pela oportunidade e confiança no desenvolvimento do trabalho. Sou muito grata e honrada de poder ter crescido profissionalmente dentro do BioMicS. ResumoO câncer de bexiga é uma neoplasia urogenital que acomete homens e mulheres, sendo que somente no Brasil 8.600 novos casos ao ano são diagnosticados. Cistoscopia transuretral é a conduta padrão no diagnóstico e acompanhamento do câncer de bexiga. Entretanto, tal procedimento é extremamente invasivo e doloroso além de ter elevado custo e não garantir todos os resultados. Assim, busca-se por marcadores moleculares que possam auxiliar no diagnóstico e progressão do câncer de bexiga, bem como diminuir a necessidade de exames invasivos no acompanhamento de pacientes tratados. Nesse sentido, a urina tem papel de destaque como fonte de biomarcadores devido principalmente ao seu caráter não invasivo.Nesse trabalho foram utilizadas duas abordagens 'ômicas': proteômica e metabolômica, para a busca de biomarcadores em urina para o diagnóstico e prognóstico do câncer de bexiga, respectivamente. Com a abordagem proteômica buscou-se apenas por biomarcadores para o diagnóstico da doença e, utilizando as técnicas de eletroforese 2-DE, OFFGEL e MS, juntamente com análise estatística multivariada, foi possível identificar 32 proteínas que apresentam-se como potenciais marcadores para o câncer de bexiga. A abordagem metabolômica foi empregada para a busca de biomarcadores para reincidência e progressão da doença. As técnicas analíticas utilizadas nessa abordagem, LC-MS e CE-MS, mostraram-se complementares e, dos resultados obtidos com ambas e avaliados com análise estatística multivariada foi possível indicar 19 metabólitos para reincidência e 23 metabólitos para progressão do câncer de bexiga.Assim, neste trabalho explorou-se como as ciências 'ômicas', a qual abrange técnicas analíticas de high-throughput, estatística multivariada e ferramentas de bioinformática auxiliando na descoberta de potenciais biomarcadores não invasivos para o diagnóstico e prognóstico do câncer de bexiga. Portanto, o presente estudo foi de grande importância e relevância à medida que ilustrou como tais técnicas podem potencialmente auxiliar no diagnóstico e prognóstico de doenças e contribuir para tratamentos personalizados no futuro, indicando ...

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Location of the photosynthetic carbon metabolism in microcompartments and separated phases in microalgal cells

Launay,

Avilan,

Gérard

et al. 2023

FEBS Letters

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Carbon acquisition, assimilation and storage in eukaryotic microalgae and cyanobacteria occur in multiple compartments that have been characterised by the location of the enzymes involved in these functions. These compartments can be delimited by bilayer membranes, such as the chloroplast, the lumen, the peroxisome, the mitochondria or monolayer membranes, such as lipid droplets or plastoglobules. They can also originate from liquid–liquid phase separation such as the pyrenoid. Multiple exchanges exist between the intracellular microcompartments, and these are reviewed for the CO2 concentration mechanism, the Calvin–Benson–Bassham cycle, the lipid metabolism and the cellular energetic balance. Progress in microscopy and spectroscopic methods opens new perspectives to characterise the molecular consequences of the location of the proteins involved, including intrinsically disordered proteins.

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LC-MS-based metabolomics

Cited by 466 publications

References 88 publications

Salivary Metabolomics and Oral Carcinogenesis

Salivary Metabolomics and Oral Carcinogenesis

Avaliação analítica de potenciais biomarcadores para câncer de bexiga em urina

Location of the photosynthetic carbon metabolism in microcompartments and separated phases in microalgal cells

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