LC/MS-based lipidomics to characterize breed-specific and tissue-specific lipid composition of chicken meat and abdominal fat

Li, Jingjing; Li, Zhiqiang; Ran, Jin-Shan; Yang, Chaowu; Lin, Zhongzhen; Liu, Yiping

doi:10.1016/j.lwt.2022.113611

Cited by 19 publications

(25 citation statements)

References 46 publications

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“…PUFAs are predominantly deposited in the glycerophospholipid of muscle, rather than free fatty acids [23,24]. The higher abundance of PUFA-containing glycerophospholipids in IMF could elucidate its bene cial roles in imparting a characteristic avor and nutritional value to meat [12]. Therefore, exploring the important candidate gene markers for speci c PUFA-containing glycerophospholipid molecules would contribute to a more thorough understanding of IMF deposition and poultry meat quality.…”

Section: Discussionmentioning

confidence: 99%

“…In our recently published study, the liquid chromatography (LC)-mass spectrometry (MS)-based lipidomics was used to characterize the comprehensive lipid composition of abdominal region and breast muscle. A total of 209 lipid molecules were commonly determined as the maker candidates for AF and IMF among three chicken breeds [12]. The signi cantly higher PUFA-enriched glycerophospholipids metabolites, such as PC (18:3e/19:2), PC (18:4e/21:2), PC (22:6e/17:2), PE (18:2e/22:6), and PE (18:2e/22:5), elucidated the underlying reasons why IMF could improve meat avor and nutritional value [12].…”

Section: Introductionmentioning

confidence: 99%

“…A total of 209 lipid molecules were commonly determined as the maker candidates for AF and IMF among three chicken breeds [12]. The signi cantly higher PUFA-enriched glycerophospholipids metabolites, such as PC (18:3e/19:2), PC (18:4e/21:2), PC (22:6e/17:2), PE (18:2e/22:6), and PE (18:2e/22:5), elucidated the underlying reasons why IMF could improve meat avor and nutritional value [12]. However, further investigation is required to combine these lipid metabolites with underlying molecular mechanisms to regulate and optimize body fat distribution in chickens.…”

Section: Introductionmentioning

confidence: 99%

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Molecular regulation of differential lipid molecule accumulation in intramuscular fat and abdominal fat of chicken

Huang

Wu³

et al. 2022

Preprint

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Background Reducing abdominal fat (AF) accumulation and increasing the level of intramuscular fat (IMF) simultaneously is a major breeding goal in poultry industry. Results To explore the different molecular mechanisms between AF and IMF, gene expression profiles in the breast muscle (BM) and AF among three chicken breeds were analyzed. A total of 4737 shared DEGs were identified between BM and AF, of which 2602 DEGs were upregulated and 2135 DEGs were downregulated in the BM groups compared with that in AF groups. DEGs involved in glycerophospholipid metabolism and glycerolipid metabolism were potential regulators resulting in the difference of lipid metabolite accumulation in IMF and AF. PPAR signaling pathway was the most important pathway involved in tissue-specific lipid deposition. Correlation analysis showed that most representative DEGs enriched in PPAR signaling pathway, such as FABP5, PPARG, ACOX1, GK2, were negatively correlated with PUFA-enriched glycerophospholipid molecules. Most DEGs related to glycerophospholipid metabolism, such as GPD2, GPD1, PEMT, CRLS1 and GBGT1, were positively correlated with glycerophospholipid molecules, especially DHA- and arachidonic acid (ARA)- containing glycerophospholipid molecules. Conclusion This study elucidated the molecular mechanism of tissue-specific lipid deposition and poultry meat quality.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular regulation of differential lipid molecule accumulation in intramuscular fat and abdominal fat of chicken

Huang

Wu³

et al. 2022

Preprint

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“…The β-CD:lipid ratio was determined according to the GC–MS results for lipid fractions, taking into account the main FAs in these samples. Thus, the mean molar mass for the lipid fractions was 861 g/mol (considering triglycerides as the main constituents, and a purity of 90.1%; other non-volatile constituents such as cholesterol and phospholipids were present in the chicken lipids [ 77 , 78 , 79 ]). On the other hand, β-CD had a water content of 14%, and this information was considered for the determination of the β-CD:lipid molar ratio.…”

Section: Methodsmentioning

confidence: 99%

FTIR–PCA Approach on Raw and Thermally Processed Chicken Lipids Stabilized by Nano-Encapsulation in β-Cyclodextrin

Hădărugă

Chirilă

Szakal

et al. 2022

Foods

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This study evaluated similarities/dissimilarities of raw and processed chicken breast and thigh lipids that were complexed by β-cyclodextrin, using a combined FTIR–PCA technique. Lipid fractions were analyzed as non-complexed and β-cyclodextrin-complexed samples via thermogravimetry, differential scanning calorimetry and ATR-FTIR. The lipid complexation reduced the water content to 7.67–8.33%, in comparison with the β-cyclodextrin hydrate (~14%). The stabilities of the complexes and β-cyclodextrin were almost the same. ATR-FTIR analysis revealed the presence of important bands that corresponded to the C=O groups (1743–1744 cm−1) in both the non-complexed and nano-encapsulated lipids. Furthermore, the bands that corresponded to the vibrations of double bonds corresponding to the natural/degraded (cis/trans) fatty acids in lipids appeared at 3008–3011 and 938–946 cm−1, respectively. The main FTIR bands that were involved in the discrimination of raw and processed chicken lipids, and of non-complexed and complexed lipids, were evaluated with PCA. The shifting of specific FTIR band wavenumbers had the highest influence, especially vibrations of the α(1→4) glucosidic bond in β-cyclodextrin for PC1, and CH2/3 groups from lipids for PC2. This first approach on β-cyclodextrin nano-encapsulation of chicken lipids revealed the possibility to stabilize poultry fatty components for further applications in various ingredients for the food industry.

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“…Lipidomics is a subset of metabolomics, based on liquid chromatography equipped with mass spectrometry, and is a newly emerging field of system-level analysis. , It has been widely used to explore the changes in lipid composition and molecules in various biological processes and food analytics, thus revealing potential mechanisms. , Lipidomics has become a powerful tool for groundbreaking research because lipids are a specific class of metabolite that are important in maintaining cell homeostasis . Whether C3G exerts protective effects on BPA-induced lipid metabolism remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Untargeted Lipidomics Revealed the Protective Effects of Cyanidin-3-O-glucoside on Bisphenol A-Induced Liver Lipid Metabolism Disorder in Rats

Liu

Jin

Liu

et al. 2023

J. Agric. Food Chem.

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Bisphenol A (BPA) is an estrogenic endocrine disruptor that induces metabolic disorders. Cyanidin-3-O-glucoside (C3G) has multiple functional activities and is the most abundant anthocyanin belonging to the flavonoid subgroup. This study aimed to investigate the protective effect of C3G on BPA-induced liver lipid metabolism disorder and explore its mechanism via lipidomics analysis. The results showed that C3G supplementation significantly ameliorated the serum levels of low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, total cholesterol, triacylglycerols (TG), and alanine and aspartate aminotransferase (ALT and AST). Furthermore, liver lipidomics indicated that C3G effectively facilitated the recovery of differential lipid metabolites, including TGs, phosphatidylethanolamines, phosphatidylcholines, lysophosphatidylcholines, phosphatidylinositol, cholesteryl esters, and phosphatidylserine, and reversed the levels of hepatic lipid synthesis-related genes. Our results suggest that C3G has an effective regulatory effect on BPA-induced disorders of lipid metabolism.

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LC/MS-based lipidomics to characterize breed-specific and tissue-specific lipid composition of chicken meat and abdominal fat

Cited by 19 publications

References 46 publications

Molecular regulation of differential lipid molecule accumulation in intramuscular fat and abdominal fat of chicken

Molecular regulation of differential lipid molecule accumulation in intramuscular fat and abdominal fat of chicken

FTIR–PCA Approach on Raw and Thermally Processed Chicken Lipids Stabilized by Nano-Encapsulation in β-Cyclodextrin

Untargeted Lipidomics Revealed the Protective Effects of Cyanidin-3-O-glucoside on Bisphenol A-Induced Liver Lipid Metabolism Disorder in Rats

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