LC/MS Analysis of Marine Toxins

Suzuki, Toshiyuki; Uchida, Hajime; Watanabe, Ryuichi

doi:10.1016/bs.coac.2017.08.005

Cited by 4 publications

(2 citation statements)

References 184 publications

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“…The technique of electrospray ionization (ESI) liquid chromatography-mass spectrometry (LC-MS) has proven to be one of the most powerful tools for the detection, identification and quantification of marine toxins. Suzuki et al [ 58 ] recently conducted a review on LC-MS analysis of marine toxins which concluded that similar MS/MS fragmentation patterns obtained by different equipment demonstrate that MS/MS spectra of marine toxins could be useful fingerprints for identification of toxins. Further improvements in these methods are always being considered with new combined all toxin detection methods being developed and validated for use.…”

Section: Methods For Official Control Testingmentioning

confidence: 99%

Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins

Dillon

Zaczek-Moczydlowska

Edwards

et al. 2021

Sensors

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In the past twenty years marine biotoxin analysis in routine regulatory monitoring has advanced significantly in Europe (EU) and other regions from the use of the mouse bioassay (MBA) towards the high-end analytical techniques such as high-performance liquid chromatography (HPLC) with tandem mass spectrometry (MS). Previously, acceptance of these advanced methods, in progressing away from the MBA, was hindered by a lack of commercial certified analytical standards for method development and validation. This has now been addressed whereby the availability of a wide range of analytical standards from several companies in the EU, North America and Asia has enhanced the development and validation of methods to the required regulatory standards. However, the cost of the high-end analytical equipment, lengthy procedures and the need for qualified personnel to perform analysis can still be a challenge for routine monitoring laboratories. In developing regions, aquaculture production is increasing and alternative inexpensive Sensitive, Measurable, Accurate and Real-Time (SMART) rapid point-of-site testing (POST) methods suitable for novice end users that can be validated and internationally accepted remain an objective for both regulators and the industry. The range of commercial testing kits on the market for marine toxin analysis remains limited and even more so those meeting the requirements for use in regulatory control. Individual assays include enzyme-linked immunosorbent assays (ELISA) and lateral flow membrane-based immunoassays (LFIA) for EU-regulated toxins, such as okadaic acid (OA) and dinophysistoxins (DTXs), saxitoxin (STX) and its analogues and domoic acid (DA) in the form of three separate tests offering varying costs and benefits for the industry. It can be observed from the literature that not only are developments and improvements ongoing for these assays, but there are also novel assays being developed using upcoming state-of-the-art biosensor technology. This review focuses on both currently available methods and recent advances in innovative methods for marine biotoxin testing and the end-user practicalities that need to be observed. Furthermore, it highlights trends that are influencing assay developments such as multiplexing capabilities and rapid POST, indicating potential detection methods that will shape the future market.

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Section: Methods For Official Control Testingmentioning

confidence: 99%

Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins

Dillon

Zaczek-Moczydlowska

Edwards

et al. 2021

Sensors

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“…Although still in use in some countries, it is banned in some others or restricted to specific cases, when no alternative exists. Instrumental analysis methods, such as ultra-performance liquid chromatography coupled to fluorescence detection (UPLC-FLD), liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), or liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS), have been developed for the detection of these toxins [42]. Those techniques coupled to mass spectrometry are highly sensitive, precise, and provide unequivocal confirmation of the presence of these toxins (and even different toxin analogs) in samples.…”

Section: Introductionmentioning

confidence: 99%

Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems

Reverté

Alkassar

Diogène

et al. 2023

Foods

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The emergence of marine toxins such as ciguatoxins (CTXs) and tetrodotoxins (TTXs) in non-endemic regions may pose a serious food safety threat and public health concern if proper control measures are not applied. This article provides an overview of the main biorecognition molecules used for the detection of CTXs and TTXs and the different assay configurations and transduction strategies explored in the development of biosensors and other biotechnological tools for these marine toxins. The advantages and limitations of the systems based on cells, receptors, antibodies, and aptamers are described, and new challenges in marine toxin detection are identified. The validation of these smart bioanalytical systems through analysis of samples and comparison with other techniques is also rationally discussed. These tools have already been demonstrated to be useful in the detection and quantification of CTXs and TTXs, and are, therefore, highly promising for their implementation in research activities and monitoring programs.

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Occurrence, distribution, source, and influencing factors of lipophilic marine algal toxins in Laizhou Bay, Bohai Sea, China

Chen

et al. 2020

Marine Pollution Bulletin

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LC/MS Analysis of Marine Toxins

Cited by 4 publications

References 184 publications

Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins

Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins

Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems

Occurrence, distribution, source, and influencing factors of lipophilic marine algal toxins in Laizhou Bay, Bohai Sea, China

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