LC methods for acyclovir and related impurities determination

Abstract

Acyclovir, guanine, and impurity A have been baseline separated with isocratic conditions at pH=3.0 and run time under 15 min by employing a SB-CN column from Agilent (150 mm x 4.6mm and 3.5 microm). Moreover, when run time was increased to 40 min six impurities (guanine, impurities A, F, G, Vir 3/4 and N(7)) plus acyclovir were separated in the same conditions. The mobile phase consisted of buffer A/acetonitrile 96:4 (v/v), being buffer A:25 mM H(3)PO(4) (Milli-Q H(2)O) brought to pH 3.0 with KOH. The same column provided separation for all the seven impurities described in pharmacopoeia, including impurity C, which coeluted with acyclovir in the previous conditions with a mobile phase prepared with 25 mM phosphoric acid (pH=1.8)/acetonitrile 96:4 (v/v). The method has been validated following ICH guidelines and it has demonstrated to be reliable for acyclovir and its impurities determination.

Enhanced dermal delivery of acyclovir using solid lipid nanoparticles

“…The prepared SLN formulations was centrifuged at 21,000 rpm for 45 min in a refrigerated centrifuge (Sigma 3K30, rotor no. 12150, Sigma, UK) at a temperature below 10°C, obtained supernatants were appropriately diluted and ACV was analyzed by modified HPLC method as reported by Huidobro et al [20]. This method was revalidated in which a Shimadzu HPLC system equipped with SPD-M20A PDA detector and Eclipse XDB-CN column from Agilent Technologies (250×4.6 mm, 5 μm) were used.…”

Sensitive and Rapid HPLC Quantification of Tenofovir from Hyaluronic Acid-Based Nanomedicine

“…1b) in human serum and urine (22), and the HPLC method for the determination of a nucleoside analog acyclovir and its related impurities have also been reported (23). However, to our knowledge there is no HPLC method that has been reported so far for simple and rapid quantitative estimation of TNF in nanomedicines (NMs) formulation.…”

Desenvolvimento e validação de um método analítico simples e rápido por espectroscopia UV para quantificação de aciclovir em matrizes hidrofílicas de liberação prolongada

“…Entre as distintas técnicas, citam-se espectrofotometria simples na região do visível com reação colorimétrica em meio básico, espectrofotometria UV para quantificação em comprimidos convencionais, espectrofluorometria, eletroquimiluminescência em célula galvânica e cromatografia líquida de alta eficiência (CLAE) com detector UV, sendo este último também o método descrito pela farmacopeia americana. [6][7][8][9][10][11][12] Entre os diferentes métodos descritos, a espectrofotometria na região UV do espectro eletromagnético é um dos métodos analíticos mais empregados, em função de robustez e custo relativamente baixo. 13 O controle de qualidade tornou-se uma ferramenta imprescindí-vel para a indústria farmacêutica, pois a partir dele pode-se garantir um medicamento seguro e eficaz.…”