LC-HR/MS Analysis of Lipophilic Extracts from Calendula arvensis (Vaill.) L. Organs: An Unexplored Source in Cosmeceuticals

Abstract

As part of a project aimed at promoting the use of Calendula arvensis (Vaill.) L. (field marigold, Asteraceae) phytocomplexes in cosmeceutical formulations, the chemical composition in apolar specialized metabolites is herein elucidated. Furthermore, the screening of the cytotoxicity of the apolar extracts was evaluated in order to underline their safety as functional ingredients for cosmetics. After dissection of Calendula organs (florets, fruits, leaves, bracts, stems, and roots), ultrasound-assisted maceration in n-hexane as an extracting solvent allowed us to obtain oil-like mixtures, whose chemical composition has been highlighted through a UHPLC-ESI-QqTOF-MS/MS approach. Twenty-nine metabolites were tentatively identified; different compounds, among which the well-known poly-unsaturated fatty acids, and oxylipins and phosphatides were detected for the first time in Calendula genus. The screening of the dose-response cytotoxicity of the apolar extracts of C. arvensis highlighted the concentration of 10 μg/mL as the most suitable for the formulation of cosmeceutical preparations. Sera enriched with leaf and fruit apolar extracts turned out to have the best activity, suggesting it can be used as a new source in skin care thanks to their higher content in fatty acids.

Calendula arvensis (Vaill.) L. Florets: Every Place and Season Resource? A Preliminary UHPLC-HRMS-Driven Assessment

“…As mentioned before, details about their identification are reported in a previous chemical investigation. 15 Site Variation in Floret Metabolite Content. The initial quantitative investigation was focused on giving insights into the metabolic variability related to different environmental site conditions along the coastal area, including soil features.…”

Different Extraction Procedures Revealed the Anti-Proliferation Activity from Vegetable Semi-Purified Sources on Breast Cancer Cell Lines

“…Therefore, a demethylated GPC deprotonated ion was generated, which, in turn, fragmented releasing its own fatty acid through the neutral loss of 225 Da and the corresponding product ion at m / z 224.069 ( Figures S4 and S5 ). In particular, fragments at m / z 277 could be attributed to an 18:2 fatty acid (e.g., α-linolenic or calendic acid), while the one at m / z 295 could be ascribed to its hydroxy-derivative, already described in plants belonging to Calendula genus [ 33 ]. The latter was also found in free form in both calendula and carrot samples.…”

Innovative Cosmeceutical Ingredients: Harnessing Selenosugar-Linked Hydroxycinnamic Acids for Antioxidant and Wound-Healing Properties

“…Subsequently, the absorbance at 570 nm for each well was measured using a Victor3 Perkin Elmer absorbance reader (Perkin Elmer/Wallac, Waltham, MA, USA). The cell mitochondrial redox activity inhibition (RAI, %) was calculated, compared with the untreated control cells, applying the following formula [ 21 ]: RAI (%) = ((Abs untreated cells) − (Abs treated cells)/(Abs untreated cells)) × 100 …”