LC-ESI-MS/MS method for simultaneous determination of eleven bioactive compounds in rat plasma after oral administration of Ling-Gui-Zhu-Gan Decoction and its application to a pharmacokinetics study

Ji, Bin; Zhao, Yunli; Yu, Peipei; Yang, Bin; Zhou, Ceng

doi:10.1016/j.talanta.2018.08.020

Cited by 33 publications

(22 citation statements)

References 19 publications

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“…As shown in Fig. 2, the mean plasma concentration-time profiles exhibited that plasma concentrations of 1 gradually increased and peaked at 6-12 h after oral administration in all four groups, which is consistent with previous reports (Xu et al 2013;Ji et al 2018). This slow appearance suggests the glycyrrhizin (1 with two glucuronic acid groups attached) was metabolized to 1 (aglycone of glycyrrhizin) by intestinal flora after oral administration (Krähenbühl et al 1994;Akao 2000).…”

Section: Pharmacokinetic Feature Of 1 In Vivosupporting

confidence: 90%

“…This slow appearance suggests the glycyrrhizin (1 with two glucuronic acid groups attached) was metabolized to 1 (aglycone of glycyrrhizin) by intestinal flora after oral administration (Krähenbühl et al 1994;Akao 2000). This metabolic change enables 1 to be more easily absorbed in rat plasma (Wang et al 2012;Zhou et al 2015) and explains why only much smaller amounts of glycyrrhizin were detected in plasma compared with the expected amount based on the quantity of glycyrrhizin in the methanol extract of G. uralensis (Ji et al 2018;Shan et al 2018). In the present study, the mean amounts of glycyrrhizin and 1 in the methanol extract of G. uralensis were 33.63 mg/g and 0.07 mg/g, respectively; however, similar to the results mentioned above, glycyrrhizin was detected at low concentrations in the rat plasma samples or was not detected.…”

Section: Pharmacokinetic Feature Of 1 In Vivomentioning

confidence: 99%

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Pharmacokinetic Change of Glycyrrhetinic Acid from the Roots and Rhizomes of Glycyrrhiza uralensis by Coadministration with the Rhizomes of Atractylodes japonica, A. macrocephala, or A. chinensis in an Animal Model

Kim

2020

Rev. Bras. Farmacogn.

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Atractylodes rhizomes (Atractylodes japonica Koidz. ex Kitam., A. macrocephala Koidz., and A. chinensis), Asteraceae, are widely used to treat digestive disorders and these rhizomes are commonly paired with the roots and rhizomes of Glycyrrhiza uralensis Fisch., Fabaceae, in herbal formulae, which may lead to mutual interaction in vivo. To assess the influence of three species of Atractylodes rhizomes on the pharmacokinetic features of G. uralensis, the levels of glycyrrhetinic acid in rat plasma samples were quantified using ultra-performance liquid chromatography/ion-trap mass spectrometry after intragastric coadministration. The results demonstrated that simultaneous administration of the extracts of the three Atractylodes rhizomes reduced the mean residence time and last measured concentration of glycyrrhetinic acid in plasma samples. Among the Atractylodes rhizomes, the combination of A. japonica shortened the elimination half-life and time to the maximum concentration of glycyrrhetinic acid. In conclusion, Atractylodes rhizomes, especially A. japonica, might alter the pharmacokinetic characteristics (mostly the duration in vivo) of glycyrrhetinic acid from G. uralensis when these herbs are coadministered. This study provides an understanding for species-specific influences on pharmacokinetic features in herbal combinations.

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Section: Pharmacokinetic Feature Of 1 In Vivosupporting

confidence: 90%

Section: Pharmacokinetic Feature Of 1 In Vivomentioning

confidence: 99%

Pharmacokinetic Change of Glycyrrhetinic Acid from the Roots and Rhizomes of Glycyrrhiza uralensis by Coadministration with the Rhizomes of Atractylodes japonica, A. macrocephala, or A. chinensis in an Animal Model

Kim

2020

Rev. Bras. Farmacogn.

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“…The developed method was validated according to the recommendations of the Food and Drug Administration (FDA) guideline for the validation of the bioanalytical method (Ji et al, 2018). The following characteristics were evaluated: selectivity, linearity, lower limit of quantification (LLOQ), lower limit of detection (LLOD), accuracy, precision, recovery, matrix effects, and stability.…”

Section: Methodsmentioning

confidence: 99%

Quantitative analysis and pharmacokinetic comparison of multiple bioactive components in rat plasma after oral administration of Qi‐Shen‐Ke‐Li formula and its single‐herb extracts using ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Zhou

Zheng

et al. 2020

Biomedical Chromatography

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Qi‐Shen‐Ke‐Li (QSKL), a traditional Chinese formula prepared from six herbs, has long been used for the treatment of coronary heart disease and chronic heart failure. However, the herbal combination mechanism and underlying material basis of this multi‐herbal formula are not clear. In this study, an ultra‐high‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method to simultaneously determine multiple bioactive compounds in QSKL was established and validated. Using the developed method, 18 bioactive components in rat plasma after oral administration of QSKL formula and its single herb extracts were quantified. Based on these results, pharmacokinetic (PK) parameters (T1/2, Tmax, Cmax, AUC0–48h, and AUC0–∞) of the 18 bioactive components were analyzed and compared using PKSlover 2.0 PK software. The experimental data suggested that significant changes in PK profiles were observed between the QSKL formula and its single‐herb extracts. The herbal combination in QSKL significantly influences the system exposure and the PK behaviors of the 18 bioactive components, indicating multicomponent interactions among the herbs. This study provides insight into the herbal combination mechanism and underlying material basis of the QSKL formula.

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“…EH, GR (processed) and GS in a weight ratio of 15:12:10 (15, 12 and 10 g) were refluxed in 370 ml (1: 10, w/v), 370 ml (1: 10, w/v) and 296 ml (1: 8, w/v) of deionized water for 30 min, respectively (Ji et al, 2018). The mixture was filtered through a 200‐mesh sieve after reflux.…”

Section: Methodsmentioning

confidence: 99%

Quantification of 10 bioactive components of Yazhangsan in rat plasma by LC–MS/MS and its application

Zhang

Chen

Liu

et al. 2020

Biomedical Chromatography

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Yazhangsan (YZS) is a common prescription for the treatment of cough and asthma caused by wind‐cold. The purpose of this study was to investigate the pharmacokinetic profiles of 10 bioactive components in YZS. A simple, sensitive and reliable high‐performance liquid chromatography coupled with a triple‐quadruple mass spectrometry method (LC–MS/MS) was developed and fully validated in this study for the measurement of these 10 bioactive compounds in rat plasma. One‐step protein precipitation method using methanol was applied to the treatment of rat plasma samples. Chromatographic separation was conducted on a C18 column by gradient elution, and water (containing 0.1% formic acid) and acetonitrile were chosen as the mobile phase. The analytes were quantified by using a mass spectrometer in multiple reaction monitoring scanning mode, and electrospray ionization was performed in positive and negative ion modes. The established method met the requirements for the quantification of these 10 bioactive compounds in biological samples, and it was successfully applied to the pharmacokinetic study of 10 components in rats after the intragastrical administration of YZS. This study will lay a foundation for the investigation of the mechanism of action of YZS and provide useful data for the rational use of YZS in clinical.

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LC-ESI-MS/MS method for simultaneous determination of eleven bioactive compounds in rat plasma after oral administration of Ling-Gui-Zhu-Gan Decoction and its application to a pharmacokinetics study

Cited by 33 publications

References 19 publications

Pharmacokinetic Change of Glycyrrhetinic Acid from the Roots and Rhizomes of Glycyrrhiza uralensis by Coadministration with the Rhizomes of Atractylodes japonica, A. macrocephala, or A. chinensis in an Animal Model

Pharmacokinetic Change of Glycyrrhetinic Acid from the Roots and Rhizomes of Glycyrrhiza uralensis by Coadministration with the Rhizomes of Atractylodes japonica, A. macrocephala, or A. chinensis in an Animal Model

Quantitative analysis and pharmacokinetic comparison of multiple bioactive components in rat plasma after oral administration of Qi‐Shen‐Ke‐Li formula and its single‐herb extracts using ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Quantification of 10 bioactive components of Yazhangsan in rat plasma by LC–MS/MS and its application

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