LC determination of praziquantel in human plasma

Ridtitid, Wibool; Wongnawa, Malinee; Mahatthanatrakul, Werawath; Punyo, Jarurat; Sunbhanich, Methi

doi:10.1016/s0731-7085(01)00605-7

Cited by 26 publications

(19 citation statements)

References 8 publications

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“…The previous HPLC methods described [6,[8][9][10][11][12][13][14][15] are all based principally on the method developed by Xio et al [16]. However, those methods are rather time-consuming as they involve three-step liquid-liquid extraction procedure.…”

Section: Discussionmentioning

confidence: 99%

“…A number of analytical methods have been reported for determination of praziquantel in human and animal biological fluids and tissue organ extracts. These methods involve radiometric assay [2], fluorometric assay [3], enzyme-linked immunosorbent assay [4], thin-layer chromatography (TLC), gas chromatography [5,7] and high-performance liquid chromatography (HPLC) [6,[8][9][10][11][12][13][14][15]. Most of the HPLC with UV detection methods described previously are based principally on the method developed by Xio et al [16].…”

Section: Introductionmentioning

confidence: 99%

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A high-performance liquid chromatographic method for determination of praziquantel in rat plasma: Optimization and application to pharmacokinetic study

Shah

Dey

Basak

et al. 2017

Proceedings of the 21st International Electronic Conference on Synthetic Organic Chemistry

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The precision of the method based on within-day repeatability and reproducibility (day-to-day variation) was within 15% (relative standard deviation: R.S.D. should be less than 15 according to CDER guidance for Bio-analytical Method Validation). Good accuracy was observed for both the intra-day or inter-day assays, as indicated by the minimal deviation of mean values found with measured samples from that of the theoretical values (below ±15%). Limit of Quantitation (LOQ) was

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A high-performance liquid chromatographic method for determination of praziquantel in rat plasma: Optimization and application to pharmacokinetic study

Shah

Dey

Basak

et al. 2017

Proceedings of the 21st International Electronic Conference on Synthetic Organic Chemistry

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“…From the very beginning different analytical methods were developed by different researchers and reported its level in animals and humans from a biological fluids (plasma, serum), tissue organ extracts. Some of the analytical methods, which include radiometric assay [2] , fluorometric assay [3] , enzyme-linked immunosorbent assay (ELISA) [4] , thinlayer chromatography (TLC), gas chromatography (GC) [5,6] , high-pressure liquid chromatography (HPLC) [7,[8][9][10][11][12][13][14] .…”

Section: Research Papermentioning

confidence: 99%

High-Performance Liquid Chromatography Determination of Praziquantel in Rat Plasma; Application to Pharmacokinetic Studies

Dey¹,

Ghosh²,

Rangra³

et al. 2017

pharmaceutical-sciences

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A very simplistic, selective, sensitive, and reproducible procedure based on a reversed-phase highperformance liquid chromatography was used and developed for the determination of praziquantel in rat plasma. For the separation of praziquantel from the internal standard, diazepam on an Enable, C18 column (250×4.6 mm, 5 µm particle size), with the retention time of 6.4, 8.5 min, respectively. For praziquantel with UV detector at 225 nm. The mobile phase was a mixture of acetonitrile:water in a ratio of (60:40 v/v), running through the column at the flow rate of 1 ml/min. Sample preparation of 200 µl of plasma was done by a protein precipitation by using perchloric acid. Calibration curve was found to be linear with correlation coefficients (r 2 ) is 0.9989 prepared in plasma at the concentrations of 5 to 1000 ng/ml. The precision of the above method was based on inter-day, and intra-day repeatability, and reproducibility (day-to-day variation) were found to be within the limit of 15%. Limit of quantification was accepted and found to be 5 ng/ml using 200 µl samples. The method appears to be robust and has been applied to a pharmacokinetic study of praziquantel in three groups of rats with a single oral dose of 40 mg/kg body weight.

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“…Evaluation and the determination of various pharmacokinetic parameters of ABZ and PRQ, the method has been developed which is simple, selective and sensitive analytical method for Limit of Quantitation [LOQ] determination in plasma samples for different metabolites. A number of High-performance liquid chromatographic [HPLC] system [20][21][22][23][24][25][26][27][28][29] and capillary electrophoresis methods [CE] [30][31] has been developed for the estimation of ABZ & PRQ. A recent advances method has been developed for the estimation of ABZ, ABZSO, ABZSO2 & PRQ in trans form by tandem mass spectrophotometry [LC-MS/MS].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of albendazole and praziquantel in rat plasma by HPLC-UV

Shah

Dey

Kant

et al. 2017

Proceedings of the 21st International Electronic Conference on Synthetic Organic Chemistry

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INTRODUCTIONThe most common helminthic disease of the nervous system is nee is the most common helminthic disease of the nervous system, is neurocysticercosis, which is considered as a serious public health problem in developing countries of Latin America, Asia and Africa [1][2][3]. The use of both the drugs Albendazole [ABZ] and Praziquantel [PRQ] consider an effective against the cystic larvae for the treatment of neurocysticercosis over the last 20 years with the use of both the drugs ABZ & PRQ. ABZ has been found an effective drug of choice than PRQ, but this persistence of cysts even after continuous use of ABZ [2]. For the patients, who were the persistence of cyst after the use of ABZ, an alternative treatment therapy was developed where there was simultaneous use of PRQ and ABZ has been evaluated [4][5]. Albendazole and Praziquantel in combination were used extensively for human hydatid disease [6][7][8][9]. Albendazole [ABZ] is an inactive moiety but it readily gets metabolized to an active metabolite Albendazole sulfoxide [ABZSO] then this ABZSO is then further metabolized to an inactive metabolite Albendazole sulfone [ABZSO2] [10]. As ABZ is metabolized extensively, therefore, the plasma concentration of ABZ is also very low as a result the pharmacokinetic studies were been done using Albendazole sulfoxide [ABZSO] as an active metabolite and Albendazole sulfone [ABZSO2] as an inactive metabolite [11][12][13][14][15]. Metabolism of Praziquantel [PRQ] occurs giving a number of hydroxylated metabolites [16][17][18], among which the active metabolite is mainly trans-4-hydroxypraziquantel [TRANS], an active metabolite [19]. Evaluation and the determination of various pharmacokinetic parameters of ABZ and PRQ, the method has been developed

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LC determination of praziquantel in human plasma

Cited by 26 publications

References 8 publications

A high-performance liquid chromatographic method for determination of praziquantel in rat plasma: Optimization and application to pharmacokinetic study

A high-performance liquid chromatographic method for determination of praziquantel in rat plasma: Optimization and application to pharmacokinetic study

High-Performance Liquid Chromatography Determination of Praziquantel in Rat Plasma; Application to Pharmacokinetic Studies

Simultaneous determination of albendazole and praziquantel in rat plasma by HPLC-UV

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