LC–DAD/ESI–MS/MS characterization of phenolic constituents in Rosa canina L. and its protective effect in cells

Abstract

In an aim to prove the efficiency of polyphenols of Rosa canina fruits in promoting human health. A methanolic extract of R. canina fruits was prepared by successive maceration with solvents of increasing polarity. The polyphenol composition was analyzed by HPLC–DAD–ESI–MS. The biological activity of this extract on SH‐SY5Y cells and HepG2 cells was then studied. The antioxidant activity was tested by various in vitro tests such as DPPH‐radical‐scavenging activity, FRAP assay, hydroxyl radical scavenging assay and total antioxidant capacity. The subacute toxicity of R. canina was tested on female rats by repeated intraperitoneal administration of various doses. The phenolic profiles showed 25 antioxidants distributed into three classes of phenolic compounds: glycosylated and agglomerated flavonoids/isoflavonoids, tannins and phenanthrenes. Qualitative phytochemical analyses showed that this extract lacks alkaloids. The methanolic extract of R. canina fruits has a total antioxidant capacity of 82.69 ± 1.18 μg EAA/mg of methanol extract and the IC50 of the methods used is in the following increasing order: FRAP assay (61.88 μg/ml), then hydroxyl radical scavenging assay (67.45 μg/ml) and then DPPH radical‐scavenging activity (129.81 μg/ml). The extract of R. canina did not cause any phenotypic signs of toxicity or mortality during and after treatment. The LD50 was >5,000 mg/kg, hence, R. canina was considered nontoxic. An in vivo study proved the protective effect of R. canina against cardiac and hepato‐renal toxicities. These results drew the importance of a healthy diet, where diets rich in R. canina fruits can be used as a rich natural source of antioxidants and anticarcinogenic phenolic compounds.

Effect of a Combination of Rosa canina Fruits and Apple Cider Vinegar against Hydrogen Peroxide-Induced Toxicity in Experimental Animal Models

“…e hepatoprotective effect of apple vinegar and Rosa canina has been reported in previous studies [12,23,40,41].…”

“…e ability of Rosa canina to fight free radicals in vivo was confirmed by Fetni et al [40]. Researchers evoked that the extract of RC exhibits a high protective effect against cardiac and hepatorenal toxicities [40].…”

“…e ability of Rosa canina to fight free radicals in vivo was confirmed by Fetni et al [40]. Researchers evoked that the extract of RC exhibits a high protective effect against cardiac and hepatorenal toxicities [40]. In addition, Rosa canina stabilizes and maintains membrane cell integrity by correcting the sorting of membrane-associated proteins [44].…”

“…Moreover, the beneficial properties of both natural products could be due to their richness in bioactive components particularly syringic acid, gallic acid, caffeic acid, p-coumaric acid, p-hydroxybenzoic acid, and catechin for apple vinegar [25], and daidzein 7-O-glucoside, dalspinin-7-O-β-D-galactopyranoside, naringenin 5,7-di-O-galactoside, kaempferol 3-O-rutinoside, syringetin-3-glucoside, eriodictyol-7-O-glucoside, isorhamnetin-3-O-rutinoside, and so on, for Rosa canina [40]. e synergetic effect of natural products was previously investigated in our previous reports and proved its ability to prevent numerous ailments associated with oxidative stress [29][30][31].…”

Effect on Nutritional and Functional Characteristics by Encapsulating Rose canina Powder in Enriched Corn Extrudates

“…Botelho et al [69] reported that rosehip could also be a source of condensed and hydrolyz-able tannins, from which oligomeric procyanidins were identified by Guimarães et al [70], and ellagitannins reported by Fecka et al [71]. Fetni et al [72] also reported that rosehip methanol extract could have moderate levels of tannins. According to Rauf et al [73], tannins could bind the mineral nutrient and therefore interfere in the digestion and absorption processes.…”