LC-DAD and LC–MS–MS Analysis of Piperidine Alkaloids of Lobelia inflata L. (In Vitro and In Vivo)

Kursinszki, L.; Ludányi, Krisztina; Szöke, Éva

doi:10.1365/s10337-008-0628-0

Cited by 18 publications

(35 citation statements)

References 11 publications

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“…The concentration of lobeline in samples was calculated from its peak area by use of the calibration plot. Validation studies proved that both the repeatability of the method and the recovery were good (Kursinszki et al 2008). The amounts of lobeline derivatives norlobeline, norlobelanine, and lobelidine were expressed in lobeline.…”

Section: Methodsmentioning

confidence: 82%

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Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L.

Bányai

Vojnich

Máthé

et al. 2014

In Vitro Cell.Dev.Biol.-Plant

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Lobelia inflata L. is a medicinally important species that contains numerous piperidine alkaloids. The main alkaloid lobeline is used as a respiratory stimulant. Recently, there have been studies that report on its effect on the central nervous system, drug abuse, and multidrug resistance. We have studied the piperidine alkaloid formation of in vitro cultivated L. inflata on solid Murashige and Skoog medium. With the aim of increasing the anti-addictive lobeline production, we have investigated the effect of changing MgSO 4 levels of the medium. The compounds (−)-lobeline, norlobelanine, lobelanidine-(−)-8-ethyl-10-phenyl-norlobelionol, (−)-8-ethyl-10-phenyl-lobelionol, norlobeline, lobelidine, and lobelanine were identified by HPLC-tandem mass spectrometry (MS/MS). We studied the effect of changing the MgSO 4 levels on the content of lobeline and its derivatives for in vitro cultivated L. inflata. The highest lobeline content was measured in roots cultured on 185 mg L −1 MgSO 4 -containing medium; this value was 2.5 times higher (232.4 μg g −1 ) than in the control cultures. The greatest (fourfold) increase was observed in lobelidine (71.61 μg g −1 ). Addition of MgSO 4 at 740 mg L −1 resulted in the highest lobeline contents in the herba (aerial tissues; 267.8 μg g −1 ), whereas maximum lobeline production was achieved by a further increase in the MgSO 4 level (1,480 mg L −1 ), due to an intensive increase in biomass production. A slight increase was observed in the amount of lobeline derivatives in the herba grown in 740 mg L −1 MgSO 4 -containing medium. The most significant increase (25%) was recorded in the level of norlobelanine.

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Section: Methodsmentioning

confidence: 82%

“…After air-drying of the cartridge, the alkaloid containing fraction was eluted from the tube with 2×2.5 mL methanol. On basis of the HPLC determination, the recovery of lobeline from the SPE step was total (Kursinszki et al 2008).…”

Section: Methodsmentioning

confidence: 99%

Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L.

Bányai

Vojnich

Máthé

et al. 2014

In Vitro Cell.Dev.Biol.-Plant

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“…The alkaloid containing fraction was eluted with 2 × 2.5 mL methanol. According to Kursinszki et al [10] the recovery of lobeline from the SPE step was total, determined by HPLC.…”

Section: Methodsmentioning

confidence: 99%

“…Analysis was performed on an Agilent 6410 Triple Quad system using electrospray ionization (ESI) in positive mode. Chromatographic conditions were the same as described earlier by Kursinszki et al [10]. The eluent was 30 mM ammonium format (pH 2.80); injection volume: 10 μL; 40% eluent could flow into the mass spectrometer (solvent splitting).…”

Section: Citationmentioning

confidence: 99%

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Increasing the Anti-Addictive Piperidine Alkaloid Production of In Vitro Micropropagated Indian Tobacco by Nitrate Treatments

Vojnich¹,

Bányai²,

Máthé³

et al. 2017

J Plant Biochem Physiol

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High Pressure Liquid Chromatography Analysis of Plant Alkaloids

Oliveira

2014

Encyclopedia of Analytical Chemistry

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Alkaloids have been used in medicine for centuries because of their biological properties and are obtained mainly from plants. Many have beneficial pharmacological properties, some are psychotropic, and others are toxic to humans and animals. These properties have led to the development of methods for their analysis in plant materials and pharmaceutical products. HPLC (high performance liquid chromatography) is the technique of choice by many analysts for the analysis of alkaloids in various scientific areas. The continuous development of new and more efficient stationary phases and detection systems has provided the conditions for the successful analysis of various classes of alkaloids. This chapter shows examples of HPLC analysis of important classes of alkaloids. Most examples involve pharmacologically useful alkaloids. Examples of toxic and psychotropic alkaloids are also covered in separate sections. The analytical conditions are shown in tabular form, and the data provided in each example may be sufficient as a starting point for the practical analyst.

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LC-DAD and LC–MS–MS Analysis of Piperidine Alkaloids of Lobelia inflata L. (In Vitro and In Vivo)

Cited by 18 publications

References 11 publications

Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L.

Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L.

Increasing the Anti-Addictive Piperidine Alkaloid Production of In Vitro Micropropagated Indian Tobacco by Nitrate Treatments

High Pressure Liquid Chromatography Analysis of Plant Alkaloids

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