LC Assay for a HIV Tablet Containing Emtricitabine, Tenofovir Disoproxil Fumarate and Rilpivirine

Abstract

A liquid chromatographic method with UV detection was developed for the assay of a tablet for HIV (human immunodeficiency virus) treatment containing three active components, which are emtricitabine, tenofovir disoproxil fumarate and rilpivirine. A Hypersil BDS-C18 column was used as stationary phase and the assay was performed with gradient elution using mobile phases containing acetonitrile, 0.2 M potassium dihydrogen phosphate and water. Dimethyl sulfoxide-distilled water (1:1) was used as solvent for the active components. The method proved to be selective, linear, repeatable, sensitive and easy to perform.

Determination of antiretroviral drugs for buyers’ club in Switzerland using capillary electrophoresis methods

“…Different separative techniques, such as HPLC–UV [12–17], LC–MS [18,19], and high‐performance thin‐layer chromatography [20], have been introduced for the analysis of antiretroviral drugs.…”

Simultaneous determination and validation of emtricitabine, rilpivirine and tenofovir from biological samples using LC and CE methods

“…TEN is recovered at 70–80% of the oral dose from urine in the unchanged form (http://www.rxlist.com/viread-drug/clinical-pharmacology.htm) and 6.1% RIL is excreted unchanged in urine (http://www.rxlist.com/edurant-drug/clinical-pharmacology.htm). A literature review reveals some chromatographic methods developed for this drug combination (Pendela et al, ; Prabhakar Reddy, Chandra Teja, Ashraf Sultana, Vijayalakshmi, & Nalluri, ; Pranitha et al, ; Prasad Panigrahy & Kumar Reddy, ). Most of them successfully separate the combinations in about 10–15 min (Khan, Venkateswara, Pulla, Kumar Sudam, & Sujana, ; Pendela et al, ; Prabhakar Reddy et al, ; Pranitha et al, ), although Prasad Panigrahy and Kumar Reddy () achieved it in an 8 min run time using reversed‐phase HPLC.…”

“…A literature review reveals some chromatographic methods developed for this drug combination (Pendela et al, ; Prabhakar Reddy, Chandra Teja, Ashraf Sultana, Vijayalakshmi, & Nalluri, ; Pranitha et al, ; Prasad Panigrahy & Kumar Reddy, ). Most of them successfully separate the combinations in about 10–15 min (Khan, Venkateswara, Pulla, Kumar Sudam, & Sujana, ; Pendela et al, ; Prabhakar Reddy et al, ; Pranitha et al, ), although Prasad Panigrahy and Kumar Reddy () achieved it in an 8 min run time using reversed‐phase HPLC. Two other HPLC studies were found in the literature by Venkatesan et al Both were published in 2014 (Venkatesan & Kannappan, ; Venkatesan, Kannappan, & Mannemala, ).…”