LB1597 Palmitoyl-KVK-L-ascorbic acid inhibits melanogenesis in B16F1 cells through the down-regulation of tyrosinase and MITF

Abstract

Vitiligo is an acquired skin disorder characterized by the loss of pigment cells from the epidermis. We found there were abundant SENP proteins couple with the abnormal chemokines in vitiligo lesions. SUMOylation is a reversible process that is catalyzed by the enzymes and reversed by a family of Sentrin/SUMO-specific proteases(SENPs). Interferon-g (IFN-g) triggers keratinocytes for inflammation response by activating the intracellular JAKÀSTAT1 signaling. By analyzing the expression profiles of genes from SENP1-/-cells, we found the down-regulation of IFN target genes in SENP-/-cell in comparison with SENP+/+. Therefore, we proprse that SENP1 plays an important role in IFN induce signal and chemokines activation. We found the down-regulation of IFN-induced genes in SENP1-/-or SENP1-silenced KCs, and the decrease in activation of Jak2 and Stat1. SENP1-deficient KCs show defects in IFN-g signaling and type 1 T helper cells Chemokines activation. Meanwhile, we also find that SENP1 expression is induced by IFN-g. PTP1B in SENP1-deficient KCs is highly SUMOylated, which reduces PTP1B-inducedde-phosphorylation of STAT3. Activated STAT3 then suppresses STAT1 activation via SOCS3 induction in SENP1-deficient keratinocytes. Accordingly, SENP1-deficient keratinocytes show reduced ability to resist UVB. These results reveal a crucial role of SENP1-controlled STAT1 and STAT3SUMOylation balance in keratinocyte function.

Detection of Ascorbic Acid Using Green Synthesized Carbon Quantum Dots

“…The abnormal concentration of AA can be related to various kinds of diseases including scurvy, mental disorder, cancer, cold, AIDS, and digestive disorder [1][2][3][4][5]. In addition, AA has been found to participate in a lot of life processes, such as cell division, iron absorption, acceleration of collagen synthesis, melanogenesis inhibition, and metabolism [6][7][8][9]. Since AA is so important, it is of great significance to develop a rapid, sensitive analytical method for the detection of AA.…”