Lazaroid U-74389G inhibits the osteoblastic differentiation of IL-1β-indcued aortic valve interstitial cells through glucocorticoid receptor and inhibition of NF-κB pathway

Sun, Fuqiang; Shi, Jing; Chen, Si; Deng, Cheng; Hu, Xingjian; Li, Huadong; Geng, Li; Liu, Yi; Dong, Nianguo

doi:10.1016/j.jsbmb.2015.05.001

Cited by 16 publications

(10 citation statements)

References 46 publications

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“…It is well known in the literature that IL‐1β increases IL‐6 and IL‐8 secretion 14,40‐42 . In a recent study, human preadipocytes were shown to be highly responsive to stimulation with IL‐1β, resulting in release of various cytokines, including TNF‐α, IL‐6, IL‐8, and IL‐10 40 .…”

Section: Discussionmentioning

confidence: 99%

“…38,39 It is well known in the literature that IL-1b increases IL-6 and IL-8 secretion. 14,[40][41][42] In a recent study, human preadipocytes were shown to be highly responsive to stimulation with IL-1b, resulting in release of various cytokines, including TNF-a, IL-6, IL-8, and IL-10. 40 Because protein production detection is paramount, results showed significant decrease in secretion of IL-6 and IL-8 induced by IL-1b in HGF and HPLF when AT1R was silenced.…”

Section: Discussionmentioning

confidence: 99%

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Angiotensin II Type 1 Receptor Knockdown Impairs Interleukin‐1β‐Induced Cytokines in Human Periodontal Fibroblasts

Gabriele

Morandini

Dionísio

et al. 2017

Journal of Periodontology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Angiotensin II Type 1 Receptor Knockdown Impairs Interleukin‐1β‐Induced Cytokines in Human Periodontal Fibroblasts

Gabriele

Morandini

Dionísio

et al. 2017

Journal of Periodontology

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“…We used RNA sequencing to explore the mechanism underlying the therapeutic effect of ADSCs and found that ADSCs may mediate repair by activating expression of the NF-jB signaling pathway. NF-jB activation plays a major role in the transcriptional control of acute and chronic inflammation, 46 and the complex may induce anti-inflammatory effects through this pathway. These findings suggest that ADSCs alone as well as the complex could effectively treat radiation-induced vaginal injury.…”

Section: Paracrine And/ormentioning

confidence: 99%

Healing effects of a protein scaffold loaded with adipose-derived mesenchymal stem cells on radiation-induced vaginal injury in rats

She

et al. 2020

J Int Med Res

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Objectives Cervical cancer, the most common female cancer after breast cancer, is typically treated using radiotherapy. However, pelvic radiotherapy can cause irreversible damage to the vagina, seriously affecting patients’ quality of life. In this study, protein scaffolds loaded with rat adipose-derived mesenchymal stem cells (ADSCs) were implanted into irradiated tissue to assess their healing potential. Methods We established a rat model of radiation-induced vaginal injury. Complexes (consisting of protein scaffolds loaded with ADSCs) were implanted into injury sites. Histological analysis were used to assess regeneration of the vaginal epithelium. RNA sequencing was used to study the therapeutic mechanism of the complexes. Results The complexes promoted vaginal epithelial cell regeneration, vaginal tissue repair and improved vaginal stenosis and contracture. Compared with rats transplanted with ADSCs, rats transplanted with complexes achieved better therapeutic effects. Conclusions Protein scaffold-ADSC complexes had a beneficial therapeutic effect on radiation-induced vaginal injury in rats and may serve as the basis of a novel therapeutic approach for radiation dermatitis.

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“…Antioxidant action of ALA is mediated by two essential nuclear factors: nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor kappa-light chain-enhancer of activated B cells (NF-kB) [ 5 , 6 , 7 , 8 , 9 , 10 ]. Amongst the many cytokines under transcriptional control of these NFs, IL-1β and IL-6 represent two relevant pro-inflammatory cytokines involved in pathological processes in many different tissues and cell types [ 11 , 12 , 13 , 14 , 15 ]. These two genes are of particular interest regarding our scopes, since it is already known that they are regulated by ALA and are involved in neurodegenerative processes [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Alpha-Lipoic Acid Downregulates IL-1β and IL-6 by DNA Hypermethylation in SK-N-BE Neuroblastoma Cells

et al. 2017

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Alpha-lipoic acid (ALA) is a pleiotropic molecule with antioxidant and anti-inflammatory properties, of which the effects are exerted through the modulation of NF-kB. This nuclear factor, in fact, modulates different inflammatory cytokines, including IL-1b and IL-6, in different tissues and cell types. We recently showed that IL-1b and IL-6 DNA methylation is modulated in the brain of Alzheimer’s disease patients, and that IL-1b expression is associated to DNA methylation in the brain of patients with tuberous sclerosis complex. These results prompted us to ask whether ALA-induced repression of IL-1b and IL-6 was dependent on DNA methylation. Therefore, we profiled DNA methylation in the 5’-flanking region of the two aforementioned genes in SK-N-BE human neuroblastoma cells cultured in presence of ALA 0.5 mM. Our experimental data pointed out that the two promoters are hypermethylated in cells supplemented with ALA, both at CpG and non-CpG sites. Moreover, the observed hypermethylation is associated with decreased mRNA expression and decreased cytokine release. These results reinforce previous findings indicating that IL-1b and IL-6 undergo DNA methylation-dependent modulation in neural models and pave the road to study the epigenetic mechanisms triggered by ALA.

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Lazaroid U-74389G inhibits the osteoblastic differentiation of IL-1β-indcued aortic valve interstitial cells through glucocorticoid receptor and inhibition of NF-κB pathway

Cited by 16 publications

References 46 publications

Angiotensin II Type 1 Receptor Knockdown Impairs Interleukin‐1β‐Induced Cytokines in Human Periodontal Fibroblasts

Angiotensin II Type 1 Receptor Knockdown Impairs Interleukin‐1β‐Induced Cytokines in Human Periodontal Fibroblasts

Healing effects of a protein scaffold loaded with adipose-derived mesenchymal stem cells on radiation-induced vaginal injury in rats

Alpha-Lipoic Acid Downregulates IL-1β and IL-6 by DNA Hypermethylation in SK-N-BE Neuroblastoma Cells

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