Layilin, a cell surface hyaluronan receptor, interacts with merlin and radixin

Hyaluronan and Layilin Mediate Loss of Airway Epithelial Barrier Function Induced by Cigarette Smoke by Decreasing E-cadherin

“…We confirmed that layilin specifically binds HA (39) as reported previously. What pointed toward layilin as an ideal HA receptor to be involved epithelial permeability is that it is known to interact with members of the ezrin, radixin, and moesin proteins (81) that are key regulators of cytoskeletonplasma membrane interactions in polarized cell. CD44 and RHAMM are two of the better characterized HA receptors in airway epithelial cells (19,21,(82)(83)(84), but in intact epithelium CD44 expression is restricted to the basolateral domain (83,85,86) and thus, at least initially, cannot be accessed by fragments released during luminal HA degradation.…”

Structural Basis for the Interaction between the Cytoplasmic Domain of the Hyaluronate Receptor Layilin and the Talin F3 Subdomain

“…2,3 Layilin is a widely expressed 55-kDa type 1 transmembrane protein that contains an extracellular domain homologous with the carbohydrate-recognition domain of C-type lectins. 4 The homology between layilin and selectins, which are involved in the initial adhesion of lymphocytes to endothelial cells, has led to the suggestion that layilin supports transient adhesion, which allows the subsequent assembly of more stable, although still short-lived, focal complexes (FXs). 4,5 Adhesion in FXs is mediated by the wellcharacterised integrin family of dimeric receptors 6 that bind to proteins rather than the carbohydrate components of the ECM.…”

“…4 The homology between layilin and selectins, which are involved in the initial adhesion of lymphocytes to endothelial cells, has led to the suggestion that layilin supports transient adhesion, which allows the subsequent assembly of more stable, although still short-lived, focal complexes (FXs). 4,5 Adhesion in FXs is mediated by the wellcharacterised integrin family of dimeric receptors 6 that bind to proteins rather than the carbohydrate components of the ECM. Some FXs transform into larger, more stable structures called focal adhesions (FAs) as a result of RhoA-mediated actomyosin contraction 7,8 or exposure to mechanical force.…”

Hyaluronan drug delivery systems are promising for cancer therapy because of their selective attachment, enhanced uptake, and superior efficacy

“…It is approximately a 55 kDa transmembrane protein that shares structural similarity to C-type lectins [52]. Although the structure of layilin has been found to be different from CD44 receptor [52], they share a common ligand on the extracellular and cytosolic surfaces [52,78]. The affinity of hyaluronan for the extracellular domain of layilin parallels hyaluronan-CD44 complexes [52].…”

“…On the cytosolic domain, layilin binds to merlin, radixin, and talin, which are members of the ERM family [52,78]. These interactions have a downstream effect on cell migration and tumor metastasis [52,78].…”