Layer-resolved colorectal tissues using nonlinear microscopy

Li, Lianhuang; Li, Hongsheng; Chen, Zhifen; Zhuo, Shuangmu; Feng, Changyin; Yang, Yinghong; Guan, Guoxian; Chen, Jianxin

doi:10.1007/s10103-015-1767-9

Cited by 13 publications

(7 citation statements)

References 30 publications

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“…There are four distinct peaks at 405, 511, 470, and 530 nm (red arrows in Fig. 1), where the strong fluorescence peak at 511 nm originates from elastic fibers or elastin in blood vessel wall, whereas the fluorescence peaks at 470 and 530 nm correspond to nicotinamide adenine dinucleotide hydrogen (NADH) and flavin adenine dinucleotide (FAD), respectively, in neuroendocrine tumor cells, and the 405 nm peak (half the 810 nm excitation wavelength) is responsible for collagen fibers or desmoplastic reaction induced by the neuroendocrine tumors [17,18]. Therefore, the neuroendocrine tumor can be label-freely detected by nonlinear microscopy because of these intrinsic nonlinear optical signals.…”

Section: Resultsmentioning

confidence: 99%

Nonlinear optical microscopy for label-free detection of gastrointestinal neuroendocrine tumors

Jiang

Chen

et al. 2016

Lasers Med Sci

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Neuroendocrine tumors (NETs), which are rare and slow-growing neoplasms, pose a diagnostic challenge as they are clinically silent at the time of presentation. Here, gastrointestinal neuroendocrine tumors were researched by nonlinear microscopy, and results demonstrate that this technique has the capability to identify neuroendocrine tumors in the absence of labels and can, in particular, detect rare neuroendocrine tumor cells, vascular invasion, desmoplastic reaction, and fibroelastosis induced by neuroendocrine tumors. These conclusions highlight the possibility of nonlinear optical microscopy as a diagnostic tool for label-freely differentiating neuroendocrine tumors by these histopathologic features.

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Section: Resultsmentioning

confidence: 99%

Nonlinear optical microscopy for label-free detection of gastrointestinal neuroendocrine tumors

Jiang

Chen

et al. 2016

Lasers Med Sci

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“…It is well known that collagen matrix forms the basic framework of stroma in mucosa and plays an important role in the development of cancer (Zheng et al, 2011;Li et al, 2015b). During precancerous development in normal mucosa, neoplastic cells will change the extracellular matrix.…”

Section: Discussionmentioning

confidence: 99%

Second‐harmonic imaging microscopy for identifying colorectal intraepithelial neoplasia

Jiang

Kang

et al. 2018

Journal of Microscopy

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In this study, second-harmonic imaging microscopy was used to monitor precancerous colorectal lesions at different stages. It was found that the morphology of glands and lamina propria in mucosa changes with the progression of colorectal diseases from normal to low-grade intraepithelial neoplasia to high-grade intraepithelial neoplasia and this microscopy has the ability of direct visualization of these warning symptoms. Furthermore, two morphologic variables were quantified to determine the changes of glands and collagen in lamina propria during the development of colorectal intraepithelial neoplasia. These results suggest that second-harmonic imaging microscopy has the potential in label-freely and effectively distinguishing between normal and precancerous colorectal tissues, and will be helpful for early diagnosis and treatment of colorectal diseases.

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“…The MPM system used in this work has been made description previously [29][30][31][32]. In short, the MPM images were obtained using a Zeiss LSM 510 META system (Jena, Germany) equipped with a mode-locked Ti: sapphire laser (tuning range of 700-980 nm, Mira 900-F; Coherent, Inc).…”

Section: Mpm Imaging Systemmentioning

confidence: 99%

Label-free detection of brain invasion in meningiomas by multiphoton microscopy

Fang

Cai

et al. 2018

Laser Phys. Lett.

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Brain invasion in meningiomas relates to the poor prognosis. Therefore, accurate detection and treatment of brain invasion are of particular importance. In this study, multiphoton microscopy (MPM) was introduced for label-free detection of brain invasion in meningiomas by the combination of the two-photon excited fluorescence imaging, second-harmonic generation imaging and spectral imaging. Results show that MPM has the ability to morphologically and spectrally identify brain invasion in meningiomas. What is more, the intensity ratios of the cerebral cortex over meningiomas at 475 nm, 511 nm and 540 nm were extracted as quantitative indicators for differentiating meningioma from surrounding normal cerebral cortex tissue. Together with custom-developed image processing algorithms, MPM could further identify the tumor boundary. With miniaturization and optimization of probes and optical fiber for in vivo imaging, combined with a laser ablation system, MPM would be used in an operating room for both the diagnosis and treatment of brain invasion in meningiomas.

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Layer-resolved colorectal tissues using nonlinear microscopy

Cited by 13 publications

References 30 publications

Nonlinear optical microscopy for label-free detection of gastrointestinal neuroendocrine tumors

Nonlinear optical microscopy for label-free detection of gastrointestinal neuroendocrine tumors

Second‐harmonic imaging microscopy for identifying colorectal intraepithelial neoplasia

Label-free detection of brain invasion in meningiomas by multiphoton microscopy

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