Layer‐by‐layer self‐assembly of collagen and chitosan biomolecules on polyurethane films

Abstract

Polyurethane (PU) films used in wound dressing applications often have appropriate properties. Still, surface modification is necessary to increase the biocompatibility. In this research, the surface of the PU films was modified with collagen and chitosan biomolecules through layer by layer (LbL) self‐assembly process. The PU films were synthesized from castor oil and hexamethylene diisocyanate. Then, they were treated with low‐pressure nitrogen plasma to graft with poly(acrylic acid). Before performing the LbL process, the surfaces of the PU films were modified using three different reagents, including (A) NaOH solution, (B) EDC/NHS solution, or (C) hexamethylene diamine. Then, the collagen and chitosan deposited in three and five layers on the surface. The images of FESEM, confocal microscopy, and AFM showed that the best performance of the LbL deposition process was after the surface modification with hexamethylenediamine (HMDA). The MTT assay showed that the presence of these biomolecules had boosted the proliferation of fibroblast cells. Increasing the number of the deposited layer from three to five, decreased the cell viability and antibacterial activity of the films. The obtained results propose the modification with HMDA and the deposition of three layers of collagen and chitosan on the PU films to improve its biocompatibility.

Synthesis and surface modification of sunflower oil‐based non‐isocyanate polyurethane: Physicochemical and antibacterial properties

“…One of the approaches used for the modification of films is LbL self‐assembly (LbL). This method is based on binding molecules through electrostatic forces and is controlled by the adsorption and desorption balance between anionic and cationic solutions 25 . Polyelectrolyte deposition involves simple dipping and drying steps in repetitive cycles.…”

“…This method was applied by Meng and coworkers 24 onto a coronary stent improving its anticoagulant properties, using chitosan/heparin as oppositely charged polyelectrolytes. Bahrami and coworkers 25 used a collagen‐chitosan coating by the LbL self‐assembly method to modify the surface of castor oil‐hexamethylene diisocyanate PU films. The three‐layer deposition of the coating improved the biocompatibility of the films.…”

Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

“…Materials based on collagen and chitosan films have been widely utilized, such as in biomedicine (Andonegi et al 2020;Bhuimbar et al 2019), tissue engineering (Becerra et al 2022;Pandini et al 2022), food packaging (Ahmad et al 2016) and the leather industries (Ocak 2021). CS conferred the film-forming ability of RHLC similar to other extracted collagens or recombinant collagens (Andonegi et al 2020;Bahrami et al 2020;Lu et al 2020). Adding an active constituent (such as a bacteriostatic compound) would improve the value and use of the film (Correia et al 2022).…”

Tri‐functional unit groups contained polyurethane composites with excellent antibacterial property and biocompatibility

“…These studies may have excellent antibacterial performance, but lack evaluations of biocompatibility, and complicated synthesis process are required. Besides, studies focus on biocompatibility which is achieved by introducing natural macromolecules like chitosan and collages, 24,25 meanwhile, the additional modification step is needed to attain better antibacterial functionalities.…”