Layer-by-layer self-assembly of a metallo-supramolecular coordination polyelectrolyte studied by infrared spectroscopy, microgravimetry, and X-ray reflectance

Kurth, Dirk G.; Schütte, Markus

doi:10.1002/1521-3900(200102)164:1<167::aid-masy167>3.0.co;2-m

Cited by 10 publications

(10 citation statements)

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“…Ligand and MEPE syntheses were performed according to the procedure described elsewhere. [67,68] The resulting LbL sample consists of a PEI, a PSS, and a final Fe-MEPE Layer. Before coating, the used circular glass slides were washed with ethanol and dried with compressed air.…”

Section: Methodsmentioning

confidence: 99%

“…After the application of the last layer, the samples were dried with compressed air. [67,68] The resulting LbL sample consists of a PEI, a PSS, and a final Fe-MEPE Layer.…”

Section: Methodsmentioning

confidence: 99%

“…The observed absorption bands at 335, 390, and 595 nm are assigned to intraligand π-π* transitions, d-d transitions, and the characteristic metal-to-ligand-charge-transfer (MLCT) transition. [68] Since hydrophobicity is crucial for cell growth also contact angle measurements are performed. In previous work, we showed that the layer thickness can be determined accurately from the absorption value, [15] which amounts to 25 nm and 43 nm and the molar attenuation coefficient.…”

Section: Surface Characterizationmentioning

confidence: 99%

“…[38,70] As expected, the thinnest layers are formed by layer-by-layer (LbL) coating because only nanoscaled layers (2 nm) are deposited in each deposition cycle on the substrate. [68] Since hydrophobicity is crucial for cell growth also contact angle measurements are performed. [39] The coated glass slides are incubated in phosphate buffer, rinsed thoroughly with water, and dried before the contact angles are determined.…”

Section: Surface Characterizationmentioning

confidence: 99%

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Growth and Differentiation of Myoblastic Precursor Cells on Thin Films of Metallo‐Supramolecular Coordination Polyelectrolyte (MEPE)

Belka

Weigel

Berninger

et al. 2016

Adv Materials Inter

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The interaction of cells with substrate surfaces is a key issue in tissue engineering. In a proof-of-concept study, the use of metallo-supramolecular polyelectrolytes (MEPE) as substrates for the adherent growth of eucaryotic cells is investigated. The chosen MEPE comprises iron(II), thus resulting in an overall positive charge of the surface, which is beneficial for cell adherence. Additionally, the metal ions can dissociate from the assembly thereby triggering cellular processes such as cell differentiation. As cell-based test system the well characterized cell line C2C12 is used. The investigated MEPE functions as noncytotoxic, biocompatible substrate. Additionally, the substrate positively affects the myogenic differentiation of the investigated cells. This proof-of-principle study suggests that MEPEs may be interesting as component for tissue engineering approaches

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Section: Methodsmentioning

confidence: 99%

“…After the application of the last layer, the samples were dried with compressed air. [67,68] The resulting LbL sample consists of a PEI, a PSS, and a final Fe-MEPE Layer.…”

Section: Methodsmentioning

confidence: 99%

Section: Surface Characterizationmentioning

confidence: 99%

Section: Surface Characterizationmentioning

confidence: 99%

See 2 more Smart Citations

Growth and Differentiation of Myoblastic Precursor Cells on Thin Films of Metallo‐Supramolecular Coordination Polyelectrolyte (MEPE)

Belka

Weigel

Berninger

et al. 2016

Adv Materials Inter

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“…As in our previous publication, the metallo-supramolecular coordination polyelectrolyte (MEPE) based on the ditopic ligand 1,4-bis (2,2′:6′,2′-terpyridin-4′-yl) benzene and Fe(II)-ions was used [1]. As several methods can be employed in order to deposit the positively charged Fe-MEPE on surfaces, we used the layer-by-layer deposition method and dip-coating from solution [25,26,27,28,29,30]. Due to the dynamic nature of the interaction of terpyridines and metal ions, such as Fe(II), Ni(II), or Co(II), MEPEs may represent ideal metal ion release systems.…”

Section: Introductionmentioning

confidence: 99%

Growth on Metallo-Supramolecular Coordination Polyelectrolyte (MEPE) Stimulates Osteogenic Differentiation of Human Osteosarcoma Cells (MG63) and Human Bone Marrow Derived Mesenchymal Stem Cells

2019

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Background: Culturing of cells is typically performed on standard tissue culture plates generating growth conditions, which in general do not reflect the native three-dimensional cellular environment. Recent investigations provide insights in parameters, which strongly affect the general cellular behavior triggering essential processes such as cell differentiation. The physical properties of the used material, such as stiffness, roughness, or topology, as well as the chemical composition of the cell-surface interface are shown to play a key role in the initiation of particular cellular responses. Methods: We extended our previous research, which identified thin films of metallo-supramolecular coordination polyelectrolytes (MEPEs) as substrate to trigger the differentiation of muscular precursor cells. Results: Here, we show that the same MEPEs similarly stimulate the osteogenic differentiation of pre-osteoblasts. Remarkably, MEPE modified surfaces also trigger the differentiation of primary bone derived mesenchymal stem cells (BMSCs) towards the osteogenic lineage. Conclusion: This result leads to the conclusion that these surfaces individually support the specification of cell differentiation toward lineages that correspond to the natural commitment of the particular cell types. We, therefore, propose that Fe-MEPEs may be used as scaffold for the treatment of defects at least in muscular or bone tissue.

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Nanoscopic Structure of a Metallo‐supramolecular Polyelectrolyte–Amphiphile Complex, Elucidated by X‐ray Scattering and Molecular Modeling

et al. 2003

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A combination of molecular modeling and X-ray scattering was used to elucidate the structure of the metallosupramolecular polyelectrolyte--amphiphile complex (PAC) self-assembled from FeII, 1,4-bis(2,2':6,'2"-terpyridin-4'-yl)benzene, and dihexadecyl phosphate (DHP). An approximate structure of the semi-ordered material was derived from the analysis of the X-ray scattering data. The experimental data provided sufficient input for obtaining a useful starting configuration for molecular modeling. Various models of the supramolecular architecture are presented and discussed in terms of their total energies and scattering patterns. In an iterative approach each level of the structural hierarchy was refined until satisfactory agreement of calculated and experimental scattering patterns was reached. The remarkable sensitivity of the simulated scattering curves to even the smallest structural changes at all length scales restricts the arbitrariness of modeling. The final model of PAC consists of flat lamellae of alternating strata of interdigitated DHP monolayers and nematically ordered polyelectrolyte chains.

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Layer-by-layer self-assembly of a metallo-supramolecular coordination polyelectrolyte studied by infrared spectroscopy, microgravimetry, and X-ray reflectance

Cited by 10 publications

References 0 publications

Growth and Differentiation of Myoblastic Precursor Cells on Thin Films of Metallo‐Supramolecular Coordination Polyelectrolyte (MEPE)

Growth and Differentiation of Myoblastic Precursor Cells on Thin Films of Metallo‐Supramolecular Coordination Polyelectrolyte (MEPE)

Growth on Metallo-Supramolecular Coordination Polyelectrolyte (MEPE) Stimulates Osteogenic Differentiation of Human Osteosarcoma Cells (MG63) and Human Bone Marrow Derived Mesenchymal Stem Cells

Nanoscopic Structure of a Metallo‐supramolecular Polyelectrolyte–Amphiphile Complex, Elucidated by X‐ray Scattering and Molecular Modeling

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