Layer-by-layer polysaccharide-coated liposomes for sustained delivery of epidermal growth factor

Abstract

A three-dimensional layer-by-layer (LbL) structure composed by xanthan and galactomannan biopolymers over dioctadecyldimethylammonium bromide (DODAB) liposome template was proposed and characterized for protein drug delivery. The polymers and the surfactant interaction were sufficiently strong to create a LbL structure up to 8 layers, evaluated using quartz crystal microbalance (QCM) and zeta potential analysis. The polymer-liposome binding enthalpy was determined by isothermal titration calorimetry (ITC). The bilayer of biopolymer-coated liposomes with diameters of 165 (±15)nm, measured by dynamic light scattering (DLS), and ζ-potential of -4 (±13)mV. These bilayer-coated nanoparticles increased up to 5 times the sustained release of epidermal growth factor (EGF) at a first order rate of 0.005min(-1). This system could be useful for improving the release profile of low-stability drugs like EGF.

Essential Oils-Loaded Electrospun Biopolymers: A Future Perspective for Active Food Packaging

“…e stability of liposomes is being improved through various strategies such as polymer-coated liposomes, hydrogel-liposome composites, and nanoparticlestabilized liposomes [126][127][128].…”

Chitosan-coated microvesicles: Effect of polysaccharide-phospholipid affinity on decafluorobutane dissolution

“…At-cut gold QCM (Ti/Cr 5 MHz SRS) crystal sensors were previously cleaned by immersion in a H 2 O 2 : H 2 SO 4 (1:3 v/v) solution for 5 min and rinsed with ultrapure water. The QCM sensors were modified with hexanethiol (Morita, Nukui, & Kuboi, 2006) and then 100 g mL −1 DSPC was deposited as previously described by Kaminski et al (2016). The modified crystals were then immersed in chitosan solution (1 mg mL −1 pH 3) for 10 min and subsequently washed with ultrapure water.…”

The Versatile Dioctadecyldimethylammonium Bromide

“…A three-dimensional layer-by-layer (LbL) structure composed by xanthan and galactomannan biopolymers on DODAB liposome template created a LbL structure up to eight layers, evaluated using quartz crystal microbalance (QCM) and zeta potential analysis; these bilayercoated NPs increased up to five times the sustained release of epidermal growth factor (EGF) and could be useful for improving the release profile of low-stability drugs like EGF [87].…”