Layer-by-layer fabrication of well-packed gold nanoparticle assemblies guided by a β-sheet peptide network

Abstract

In this study, we describe the first demonstration of well-ordered multilayer thin films of gold nanoparticles through a layer-bylayer (LbL) technique assisted by a polypeptide template. Anionic poly(L-glutamic acid) (PLGA)-modified gold nanoparticles were prepared as building units for LbL self-assembly. Two types of synthetic polymers, poly(L-lysine) (PLL) and poly(allylamine), were used as polycations that carry the same amino groups at the side chain, although the former is a polypeptide and the latter is a vinyl polymer. The deposition process and the resultant multilayer films were characterized by means of absorption, circular dichroism, Fourier transform infrared and atomic force microscopy measurements. Experimental results revealed that LbL multilayer properties, such as thickness, conformation of the peptide component, homogeneity and periodicity of nanoparticle layers, were significantly affected by the kind of polycations present. In particular, the combination of anionic PLGA-coated gold nanoparticles and cationic PLL induced b-sheet formation, which enabled the fabrication of a homogeneously well-packed gold nanoparticle multilayer without aggregation.

Gold nano‐decorated aligned polyurethane nanofibers for enhancement of neurite outgrowth and elongation

“…It has long been used to functionalize the different surfaces before coating with gold nanoparticles . Gold nanoparticles due to their citrate layer on the surface, they are negatively charged and can attach to positively charged surfaces via electrostatic interaction . So, in this study aligned PU nanofibers were functionalized with PLL and then immersed in gold nanoparticles solution.…”

“…28 Gold nanoparticles due to their citrate layer on the surface, they are negatively charged and can attach to positively charged surfaces via electrostatic interaction. 29 So, in this study aligned PU nanofibers were functionalized with PLL and then immersed in gold nanoparticles solution. In this way, very dense and homogeneous decoration with gold nanoparticles on nanofibers was carried out [ Fig.…”

Label-free detection of ApoE4-mediated β-amyloid aggregation on single nanoparticle uncovering Alzheimer's disease

“…2D is determination of β-sheet structure due to ApoE4 activity by CD. The parking of β-sheet of Aβ42 was higher with ApoE4 than that without ApoE4, suggesting tight β-sheet form (Higashi et al, 2010). Using in-silico analysis (molecular docking) for binding complexes of Aβ42 and ApoE isoforms (ApoE2, ApoE3, ApoE4), only ApoE4 exhibits a misfolded intermediate when jointed to Aβ42 (Luo et al, 2010).…”

Investigation of a structure of new functional peptide composite materials with gold nanoparticles

“…All these properties arise from the features of their molecular structure. Solid phase coatings based on PTs have good antifriction properties, which strongly depend on the conforma tion type [2]. Currently, the majority of investigations of PTs and polypeptides have been conducted in the field of medicine and biology, where their behavior in a liquid phase is of prime importance.…”