Layer-by-layer engineered nanocapsules of curcumin with improved cell activity

Kittitheeranun, Paveenuch; Sajomsang, Warayuth; Phanpee, Sarunya; Treetong, Alongkot; Wutikhun, Tuksadon; Suktham, Kunat; Puttipipatkhachorn, Satit; Ruktanonchai, Uracha

doi:10.1016/j.ijpharm.2015.06.040

Cited by 33 publications

(18 citation statements)

References 33 publications

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“…On the other hand, TEM and SEM provide the droplet images in a dry state, thus, smaller droplet sizes were observed. Similar results were reported by Kittitheeranun et al [44]. Additional atomic force microscopy (AFM) images are available in the Supplementary Materials, Section 2.1.…”

Section: Resultssupporting

confidence: 87%

Drug Release Properties of Diflunisal from Layer-By-Layer Self-Assembled κ-Carrageenan/Chitosan Nanocapsules: Effect of Deposited Layers

Rochín-Wong

Rosas-Durazo²,

Zavala-Rivera

et al. 2018

Polymers

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Engineering of multifunctional drug nanocarriers combining stability and good release properties remains a great challenge. In this work, natural polymers κ-carrageenan (κ-CAR) and chitosan (CS) were deposited onto olive oil nanoemulsion droplets (NE) via layer-by-layer (LbL) self-assembly to study the release mechanisms of the anti-inflammatory diflunisal (DF) as a lipophilic drug model. The nano-systems were characterized by dynamic light scattering (DLS), zeta potential (ζ-potential) measurements, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray energy dispersive spectroscopy (XEDS) and Fourier transform infrared spectroscopy (FTIR) to confirm the NE-coating with polymer layers. In addition, kinetic release studies of DF were developed by the dialysis diffusion bag technique. Mathematical models were applied to investigate the release mechanisms. The results showed that stable and suitably sized nanocapsules (~300 nm) were formed. Also, the consecutive adsorption of polyelectrolytes by charge reversal was evidenced. More interestingly, the drug release mechanism varied depending on the number of layers deposited. The nanosized systems containing up to two layers showed anomalous transport and first order kinetics. Formulations with three and four layers exhibited Case II transport releasing diflunisal with zero order kinetics. Hence, our results suggest that these polyelectrolyte nanocapsules have great potential as a multifunctional nanocarrier for drug delivery applications.

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Section: Resultssupporting

confidence: 87%

Drug Release Properties of Diflunisal from Layer-By-Layer Self-Assembled κ-Carrageenan/Chitosan Nanocapsules: Effect of Deposited Layers

Rochín-Wong

Rosas-Durazo²,

Zavala-Rivera

et al. 2018

Polymers

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“…24 The encapsulation of enzymes using CaCO 3 porous particles also contributed to better loading of enzymes and other applications. [25][26][27] However, the synthesis of enzyme-metal hybrids using unstable enzymes at 25 C is limited due to 3 days of long incubation. 1,3,28 Therefore, modication of synthesis conditions is required to improve the efficiency of unstable enzymes aer immobilization.…”

Section: Introductionmentioning

confidence: 99%

Protein–inorganic hybrid system for efficient his-tagged enzymes immobilization and its application in l-xylulose production

et al. 2017

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“…One of the strategies used to improve the stability of nanostructures in gastro conditions, is the application of a coating to prevent the hydrolysis of proteins by proteolytic enzymes (Somchue et al, 2009). Layer-by-Layer (LbL) deposition technique is one of the methods used in different templates (from hard and planar to rigid particles, and more recently in soft and porous templates such as nanohydrogels) to improve the stability, functional and mechanical properties of different structures (Boddohi, Almod ovar, Zhang, Johnson, & Kipper, 2010;Hirsj€ arvi, Qiao, Royere, Bibette, & Benoit, 2010;Kittitheeranun et al;Kotov, 2003;Sato, Yoshida, Takahashi, & Anzai, 2011;Wong, Müller, Díez-Pascual, & Richtering, 2009). LbL assembly is based on the electrostatic interaction between oppositely charged polyelectrolytes alternatively adsorbed onto an appropriate template (Decher, 2003).…”

Section: Introductionmentioning

confidence: 99%

Influence of chitosan coating on protein-based nanohydrogels properties and in vitro gastric digestibility

2016

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Chitosan coating was applied in Lactoferrin (Lf)-Glycomacropeptide (GMP) nanohydrogels by layer-bylayer coating process. A volume ratio of 10 of Lf-GMP nanohydrogels (0.2 mg mL À1 , at pH 5.0) to chitosan (1 mg mL À1 , at pH 3) demonstrated to be the optimal condition to obtain stable nanohydrogels with size of 230 ± 12 nm, a PdI of 0.22 ± 0.02 and a z-potential of 30.0 ± 0.15 mV. Transmission electron microscopy (TEM) images showed that the application of chitosan coating in Lf-GMP did not affect the spherical shape of nanohydrogels and confirmed the low aggregation of nanohydrogels in solution. The analysis of chemical interactions between chitosan and Lf-GMP nanohydrogels were performed by Fourier transform infrared spectroscopy (FTIR) and by circular dichroism (CD) that revealed that a specific chemical interaction occurring between functional groups of protein-based nanohydrogels and active groups of the chitosan was established. The effect of chitosan coating on release mechanisms of Lf-GMP nanohydrogels at acid conditions (pH 2, 37 C) was evaluated by the encapsulation of a model compound (caffeine) in these systems. Linear Superposition Model was used to fit the experimental data and revealed that Fick and relaxation mechanisms are involved in caffeine release. It was also observed that the Fick contribution increase with the application of chitosan coating. In vitro gastric digestion was performed with Lf-GMP nanohydrogels and Lf-GMP nanohydrogels with chitosan coating and it was observed that the presence of chitosan improve the stability of Lf and GMP (proteins were hydrolysed at a slower rate and were present in solution by longer time). Native electrophoreses revealed that the nanohydrogels without coating remained intact in solution until 15 min and with chitosan coating remained intact until 60 min, during gastric digestion.

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Layer-by-layer engineered nanocapsules of curcumin with improved cell activity

Cited by 33 publications

References 33 publications

Drug Release Properties of Diflunisal from Layer-By-Layer Self-Assembled κ-Carrageenan/Chitosan Nanocapsules: Effect of Deposited Layers

Drug Release Properties of Diflunisal from Layer-By-Layer Self-Assembled κ-Carrageenan/Chitosan Nanocapsules: Effect of Deposited Layers

Protein–inorganic hybrid system for efficient his-tagged enzymes immobilization and its application in l-xylulose production

Influence of chitosan coating on protein-based nanohydrogels properties and in vitro gastric digestibility

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