Layer-by-Layer-Coated Gelatin Nanoparticles as a Vehicle for Delivery of Natural Polyphenols

Shutava, Tatsiana G.; Balkundi, Shantanu; Vangala, Pranitha; Steffan, Joshua J.; Bigelow, Rebecca L.; Cardelli, James A.; O’Neal, D. Patrick; Lvov, Yuri

doi:10.1021/nn900451a

Cited by 398 publications

(269 citation statements)

References 42 publications

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“…Shutava et al utilized a polyelectrolyte layer-by-layer technique to coat EGCG over gelatin core nanoparticles. It was shown that the biological activity of EGCG, blocking the signaling pathway of the breast cancer cell line in their study, remained [42]. Therefore, the utilization of biopolymers to protect EGCG from oxidation is an essential practice for forming a drug delivery system.…”

Section: Instability Of Free-egcg In Solutionsmentioning

confidence: 99%

Synthesis of Gelatin-γ-Polyglutamic Acid-Based Hydrogel for the In Vitro Controlled Release of Epigallocatechin Gallate (EGCG) from Camellia sinensis

et al. 2013

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Abstract:The antioxidant property and other health benefits of the most abundant catechin, epigallocatechin gallate (EGCG), are limited because of poor stability and permeability across intestine. Protecting the EGCG from the harsh gastrointestinal tract (GIT) environment can help to increase its bioavailability following oral administration. In this study, EGCG was loaded to hydrogel prepared from ionic interaction between an optimized concentration of gelatin and γ-polyglutamic acid (γ-PGA), with ethylcarbodiimide (EDC) as the crosslinker. Physicochemical characterization of hydrogel was done using Fourier transform-infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). The dependence of the swelling degree (SD) of the hydrogel to the amount of gelatin, γ-PGA, EDC, swelling time and pH was determined. A high SD of the crosslinked hydrogel was noted at pH 4.5, 6.8 OPEN ACCESSPolymers 2014, 6 40 and 9.0 compared to pH 7.4, which describes pH-responsiveness. Approximately 67% of the EGCG from the prepared solution was loaded to the hydrogel after 12 h post-loading, in which loading efficiency was related to the amount of EDC. The in vitro release profile of EGCG at pH 1.2, 6.8 and 7.4, simulating GIT conditions, resulted in different sustained release curves. Wherein, the released EGCG was not degraded instantly compared to free-EGCG at controlled temperature of 37 °C at different pH monitored against time. Therefore, this study proves the potential of pH-responsive gelatin-γ-PGA-based hydrogel as a biopolymer vehicle to deliver EGCG.

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Section: Instability Of Free-egcg In Solutionsmentioning

confidence: 99%

Synthesis of Gelatin-γ-Polyglutamic Acid-Based Hydrogel for the In Vitro Controlled Release of Epigallocatechin Gallate (EGCG) from Camellia sinensis

et al. 2013

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“…There have been a number of recent reviews focusing on the encapsulation of the more widely used polyphenols, discussing their effectiveness, variations, developments and trends (Barras et al, 2009;Das, Ng, 2010;Di Mattia et al, 2009;Dube et al, 2010;Ersus, Yurdagel, 2007;Fang et al, 2006;Hu et al, 2008;Kosaraju, Dath, Lawrence, 2006;Laine et al, 2008;Liang et al, 2011;Lucas-Abellan et al, 2007;Mourtzinos et al, 2007;Shi et al, 2007;Shutava et al, 2009;Tommasini et al, 2005;Zhang, Mou, Du, 2007;Xiong et al, 2006;Yu, Huang, 2010).…”

Section: Introductionmentioning

confidence: 99%

Alginate-pomegranate peels' polyphenols beads: effects of formulation parameters on loading efficiency

Zam

Bashour

Abdelwahed

et al. 2014

Braz. J. Pharm. Sci.

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Calcium alginate beads containing pomegranate peels' polyphenol extract were encapsulated by ionic gelation method. The effects of various formulation factors (sodium alginate concentration, calcium chloride concentration, calcium chloride exposure time, gelling bath time maintaining, and extract concentration) on the efficiency of extract loading were investigated. The formulation containing an extract of 1 g pomegranate peels in 100 mL distilled water encapsulated with 3 % of sodium alginate cured in 0.05 M calcium chloride for 20 minutes and kept in a gelling bath for 15 minutes was chosen as the best formula regarding the loading efficiency. These optimized conditions allowed the encapsulation of 43.90% of total extracted polyphenols and 46.34 % of total extracted proanthocyanidins. Microencapsulation of pomegranate peels' extract in calcium alginate beads is a promising technique for pharmaceutical and food supplementation with natural antioxidants.

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“…On the other hand both the nanoparticles with and without EGCG did not inhibit HGF-induced signalling after pre-incubation of 30 min. However, at the 5 h time-point the gelatin coated nanoparticle containing EGCG blocked HGF-induced signalling, therefore demonstrating that EGCG activity was retained but released slowly (Shutava et al, 2009). Recently, curcumin nanoparticles have shown enhanced bioavailability and greater cytotoxicity against breast cancer cells.…”

Section: Novel Drug Delivery Of Natural Compoundsmentioning

confidence: 97%

Experimental Therapeutics for the Treatment of Triple Negative Breast Cancer

Dzeyk¹,

Yadav²,

Rosengren³

2011

Breast Cancer - Current and Alternative Therapeutic Modalities

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Layer-by-Layer-Coated Gelatin Nanoparticles as a Vehicle for Delivery of Natural Polyphenols

Cited by 398 publications

References 42 publications

Synthesis of Gelatin-γ-Polyglutamic Acid-Based Hydrogel for the In Vitro Controlled Release of Epigallocatechin Gallate (EGCG) from Camellia sinensis

Synthesis of Gelatin-γ-Polyglutamic Acid-Based Hydrogel for the In Vitro Controlled Release of Epigallocatechin Gallate (EGCG) from Camellia sinensis

Alginate-pomegranate peels' polyphenols beads: effects of formulation parameters on loading efficiency

Experimental Therapeutics for the Treatment of Triple Negative Breast Cancer

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