Layer-by-layer assembly of poly(l-glutamic acid)/chitosan microcapsules for high loading and sustained release of 5-fluorouracil

Yan, Shifeng; Zhu, Jie; Wang, Zhichun; Yin, Jingbo; Zheng, Yan‐Zhen; Chen, Xuesi

doi:10.1016/j.ejpb.2010.12.031

Cited by 97 publications

(57 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5-FU (MW: 130 Da) also showed a uniform cumulative release with diffusion as rate limiting step. Similar results were shown for 5-FU release from LbL-MC made of poly-L-glycolic acid -chitosan characterized by Baker-Lonsdale model (Yan et al, 2011). In case of molecules with greater molecular weight and electrostatic interactions, IM (MW: 589 Da) showed non-Fickian release model.…”

Section: Discussionsupporting

confidence: 69%

“…These were in DOI: 10.3109/10717544.2013.867381 correlation with the studies performed with similar polymers and other charged polymers (Mak et al, 2008). LbL-MC have been studied for encapsulation efficiency of small model molecules, including 5-FU (Yan et al, 2011), perylene (Manna & Patil, 2009), curcumin (Manju & Sreenivasan, 2011) ciprofloxacin hydrochloride (Mao et al, 2005), doxorubicin (Tao et al, 2007;Liu et al, 2010) and daunorubicin (Han et al, 2008). In this study, we chose small molecules with different charge.…”

Section: Discussionmentioning

confidence: 86%

See 1 more Smart Citation

Influence of charge on encapsulation and release behavior of small molecules in self-assembled layer-by-layer microcapsules

et al. 2013

View full text Add to dashboard Cite

ORIGINAL PAPERInfluence of charge on encapsulation and release behavior of small molecules in self-assembled layer-by-layer microcapsules Praveen K. Mandapalli, Suman Labala, Deekshith Vanamala, Manali P. Koranglekar, Lakshmi A. Sakimalla, and Venkata Vamsi K. Venuganti Department of Pharmacy, BITS Pilani, Hyderabad Campus, Shameerpet, Hyderabad 500078, Andhra Pradesh, India AbstractThe objective of this study is to investigate the influence of charge of model small molecules on their encapsulation and release behavior in layer-by-layer microcapsules (LbL-MC). Poly(styrene sulfonate) and poly(ethylene imine) were sequentially adsorbed on calcium carbonate sacrificial templates to prepare LbL-MC. Model molecules with varying charge, anionic -ascorbic acid, cationic -imatinib mesylate (IM) and neutral -5-fluorouracil were encapsulated in LbL-MC. Free and encapsulated LbL-MC were characterized using zetasizer, FTIR spectroscope and differential scanning calorimeter. The influence of IM-loaded LbL-MC on cell viability was studied in B16F10 murine melanoma cells. Furthermore, biodistribution of IM-loaded LbL-MC with and without PEGylation was studied in BALB/c mice. Results showed spherical LbL-MC of 3.0 AE 0.4 mm diameter. Encapsulation efficiency of LbL-MC increased linearly (R 2 ¼ 0.89-0.99) with the increase in solute concentration. Increase in pH from 2 to 6 increased the encapsulation of charged molecules in LbL-MC. Charged molecules showed greater encapsulation efficiency in LbL-MC compared with neutral molecule. In vitro release kinetics showed Fickian and non-Fickian diffusion of small molecules, depending on the nature of molecular interactions with LbL-MC. At 50 mM concentration, free IM showed significantly (p50.05) more cytotoxicity compared with IM-loaded LbL-MC. Biodistribution studies showed that PEGylation of LbL-MC decreased the liver and spleen uptake of IM-encapsulated LbL-MC. In conclusion, LbL-MC can be developed as a potential carrier for small molecules depending on their physical and chemical properties.

show abstract

Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 86%

Influence of charge on encapsulation and release behavior of small molecules in self-assembled layer-by-layer microcapsules

et al. 2013

View full text Add to dashboard Cite

show abstract

“…7 If 5-FU were carried by CTS or its derivatives, the result would be extended release, improved bioavailability, and reduced side effects. [8][9][10] Carrier agents, such as CTS, also have adhesion and biodegradability properties that give them the potential to improve drug efficacy.…”

Section: Introductionmentioning

confidence: 99%

Optimized synthesis of glycyrrhetinic acid-modified chitosan 5-fluorouracil nanoparticles and their characteristics

Cheng¹,

Chen²,

Wang³

et al. 2014

IJN

View full text Add to dashboard Cite

The nanoparticle drug delivery system, which uses natural or synthetic polymeric material as a carrier to deliver drugs to targeted tissues, has a broad prospect for clinical application for its targeting, slow-release, and biodegradable properties. Here, we used chitosan (CTS) and hepatoma cell-specific binding molecule glycyrrhetinic acid to synthesize glycyrrhetinic acid-modified chitosan (GA-CTS). The synthetic product was confirmed by infrared (IR) spectra and hydrogen-1 nuclear magnetic resonance. The GA-CTS/5-fluorouracil (5-FU) nanoparticles were synthesized by combining GA-CTS and 5-FU and conjugating 5-FU onto the GA-CTS nanomaterial. The central composite design was performed to optimize the preparation process as CTS:tripolyphosphate sodium (TPP) weight ratio =5:1, 5-FU:CTS weight ratio =1:1, TPP concentration =0.05% (w/v), and cross-link time =50 minutes. GA-CTS/5-FU nanoparticles had a mean particle size of 193.7 nm, a polydispersity index of 0.003, a zeta potential of +27.4 mV, and a drug loading of 1.56%. The GA-CTS/5-FU nanoparticle had a protective effect on the drug against plasma degrading enzyme, and provided a sustained release system comprising three distinct phases of quick, steady, and slow release. Our study showed that the peak time, half-life time, mean residence time and area under the curve of GA-CTS/5-FU were longer or more than those of the 5-FU group, but the maximum concentration (C max ) was lower. We demonstrated that the nanoparticles accumulated in the liver and have significantly inhibited tumor growth in an orthotropic liver cancer mouse model.

show abstract

“…5b, the volumetric expansion (v) of LBPAA follows the order vpH 5> vpH 8> vpH 3, and the cumulative release profiles of cyfluthrin followed the same rules. This demonstrates that pH is important not only because of its influence on the structure of the hydrogel network, but also because of its effect on the stability of the pesticide (Ya et al 2011). …”

Section: Pesticide Loading and Release In Water At 25 °Cmentioning

confidence: 99%

Controlled Pesticide Release from Porous Composite Hydrogels Based on Lignin and Polyacrylic Acid

Sun¹,

Ma²,

Gui-zhen³

et al. 2016

BioResources

View full text Add to dashboard Cite

For the controlled release of pesticides, a novel composite porous hydrogel (LBPAA) was prepared based on lignin and polyacrylic acid for use as the support frame of a pore structure for water delivery. The LBPAA was analyzed to determine its water-swelling and slow release properties. The controlled release properties of LBPAA were evaluated through experiments in relation to the cumulative release of pesticides, with particular emphasis on environmental effects and release models. The porous LBPAA hydrogel showed improved properties compared to polyacrylic acid, and could therefore be considered an efficient material for application in controlled release systems in agriculture.

show abstract

Layer-by-layer assembly of poly(l-glutamic acid)/chitosan microcapsules for high loading and sustained release of 5-fluorouracil

Cited by 97 publications

References 53 publications

Influence of charge on encapsulation and release behavior of small molecules in self-assembled layer-by-layer microcapsules

Influence of charge on encapsulation and release behavior of small molecules in self-assembled layer-by-layer microcapsules

Optimized synthesis of glycyrrhetinic acid-modified chitosan 5-fluorouracil nanoparticles and their characteristics

Controlled Pesticide Release from Porous Composite Hydrogels Based on Lignin and Polyacrylic Acid

Contact Info

Product

Resources

About