Layer-by-layer assembly of electro-active gold nanoparticle/cytochrome c multilayers

Detecting 5-morpholino-3-amino-2-oxazolidone residue in food with label-free electrochemical impedimetric immunosensor

“…In recent years, the electrochemical immunoassays have been developed based on nanomaterials, such as colloid gold (Xiao & Yu, 2010), nano-SiO 2 (Tang, Su, Tang, Ren & Chen, 2010;Wu, Chen & Liu, 2009), nano-TiO 2 (Wang, Ruan, Kanayeva, Lassiter & Li, 2008), nano-Ag (Loyprasert et al, 2008), carbon nanotube (Diakowski, Xiao, Petryk & Kraatz, 2010), Fe 3 O 4 nanoparticles (Huang et al, 2010), and nano-Pt (Huang, Wen, Jiang, Shen & Yu, 2008), all of which have attracted considerable interest in biosensor development. Among the nanomaterials mentioned above, gold nanoparticles, which have good characteristics of easy preparation, good biocompatibility and relatively large surface, are widely used to immobilize DNA (Li, Feng, Dong & Tang, 2010;Zhang, Wang & Xu, 2010), cell (Yan, Chen & Ju, 2007), cytochrome (Bonk & Lisdat, 2009;Li et al, 2009), and enzyme (Kim, Kang, Shim & Moon, 2008;Wang, Wang, Di & Tu, 2008) in the field of biological studies. Recently, gold nanoparticles have been used to immobilize the antigen or antibody onto the electrode surface to manufacture the electrochemical immunosensors (Valera, Muñiz & Rodríguez, 2010;.…”

Development of a fast and simple test system for the semiquantitative protein detection in cerebrospinal liquids based on gold nanoparticles

“…Furthermore nanocrystalline materials can be applied advantageously for sensorial bioelectrochemistry as antibody conjugates in front of the gate of field-effect transistors [29], for amperometric detection of enzyme produced hydrogen peroxide [30], for signal amplification in impedance spectroscopy [31] or for direct electron transfer within protein electrode structures [32,33]. In contact with proteins gold nanoparticles are also able to facilitate the efficient inter protein electron transfer [34].…”

Insights into Interprotein Electron Transfer of Human Cytochrome c Variants Arranged in Multilayer Architectures by Means of an Artificial Silica Nanoparticle Matrix

“…Multilayer architectures of the redox protein can be prepared through the layer-by-layer deposition of cyt c and different kinds of second building blocks, such as polyelectrolytes, 21 DNA, 22 gold, 23 and silica nanoparticles (SiNPs). 24 …”