Layer-by-Layer Assembled Gold Nanoparticles for the Delivery of Nucleic Acids

Wurster, Eva-Christina; Elbakry, Asmaa M.; Göpferich, Achim; Breunig, Miriam

doi:10.1007/978-1-62703-140-0_12

Cited by 11 publications

(7 citation statements)

References 24 publications

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“…The particle synthesis and coating protocol has already been published in detail . In brief, 30 nm gold nanoparticles (AuNP) were synthesized by citrate reduction as follows: 2 mL of 1% (w/v) aqueous sodium citrate trihydrate (Merck KGaA, Darmstadt, Germany) solution was added to a boiling solution of 0.1% (w/v) gold(III) chloride under vigorous stirring.…”

Section: Methodsmentioning

confidence: 99%

“…The particle synthesis and coating protocol has already been published in detail. 25 In brief, 30 nm gold nanoparticles (AuNP) were synthesized by citrate reduction as follows: 2 mL of 1% (w/v) aqueous sodium citrate trihydrate (Merck KGaA, Darmstadt, Germany) solution was added to a boiling solution of 0.1% (w/v) gold(III) chloride under vigorous stirring. The conditions were kept constant for 10 min until the red color of colloidal gold appeared.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Oligolayer-Coated Nanoparticles: Impact of Surface Topography at the Nanobio Interface

Wurster

Liebl

Michaelis

et al. 2015

ACS Appl. Mater. Interfaces

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Layer-by-layer coating of nanoparticles with a layer number in the single-digit range has gained increasing attention in the field of nanomedicinal research. However, the impact of using various polyelectrolytes on oligolayer formation and, more importantly, their influence on the interaction with the biological system has not often been considered in the past. Hence, we investigated the polyelectrolyte deposition profiles and resulting surface topographies of up to three polyelectrolyte layers on a flat gold sensor surface using three different polycations, namely, poly(ethylene imine) (PEI), poly(allylamine hydrochloride) (PAH), and poly(diallylammonium chloride) (PD), each in combination with poly(styrenesulfonate) (PSS). Surface plasmon resonance spectroscopy and atomic force microscopy revealed that the PEI/PSS pair in particular showed a so-called overshoot phenomenon, which is associated with partial polyelectrolyte desorption from the surface. This is also reflected by a significant increase in the surface roughness. Then, after having transferred the oligolayer assembly onto nanoparticles of ∼32 nm, we realized that quite similar surface topographies must have emerged on a curved gold surface. A major finding was that the extent of surface roughness contributes significantly to the fashion by which the oligolayer-coated nanoparticles interact with serum proteins and associate with cells. For example, for the PEI/PSS system, both the surface roughness and protein adsorption increased by a factor of ∼12 from the second to third coating layer and, at the same time, the cell association massively decreased to only one-third. Our study shows that surface roughness, along with other particle properties such as size, shape, zeta potential, and hydrophobicity, is another decisive factor for nanoparticles in a biological context, which has indeed been discussed previously but has not to date been investigated for oligolayers.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Oligolayer-Coated Nanoparticles: Impact of Surface Topography at the Nanobio Interface

Wurster

Liebl

Michaelis

et al. 2015

ACS Appl. Mater. Interfaces

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“…Among these strategies, the layer-by-layer (LbL) technique is a versatile methodology to construct functional surfaces by alternate deposition of polycation and polyanion [5]. The LbL approach can be used to control the buildup of multilayer coatings of different architectures and functions on the nanometer scale [6,7]. The simplicity of LbL film formation enables one to design a variety of custom-designed materials with assumed antimicrobial features.…”

Section: Introductionmentioning

confidence: 99%

Multilayered Curcumin-Loaded Hydrogel Microcarriers with Antimicrobial Function

et al. 2022

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The design of multifunctional microcarriers has attracted significant attention because they combine various functions within a single system. In this study, we developed a set of multilayered hydrogel microcarriers, which were first loaded with chemotherapeutic curcumin (CUR), then, using the layer-by-layer (LbL) technique, coated through a polyelectrolyte shell consisting of chitosan (CHIT) or poly(allylamine hydrochloride) (PAH). As an outer layer with antimicrobial function, newly synthesised alkylene quaternary ammonium salt functionalised polyelectrolytes (A-QAS-PEs) were applied. For this purpose, poly(acrylic acid) (PAA) was decorated with different hydrophobic side chains (n-hexane and n-dodecane side entities) and different degrees of substitution (m) of quaternary ammonium groups (abbreviated as PAA-C(O)O-(CH2)n-N+(CH3)3(m); n = 6, 12; m = 8–14%). The grafting approach of PAA with the alkylene quaternary ammonium salt moiety was performed under mild reaction conditions using Steglich esterification followed by quaternisation. The structure of antimicrobial decorated PAA was confirmed by 1H NMR and FTIR, and the mean diameter of all multifunctional microparticles was characterised by SEM. The viscoelastic properties of the functional layers were studied using quartz crystal microbalance with a dissipation (QCM-D). The release of CUR from the microcarriers was described using a hybrid model, i.e., a combination of first-order kinetics and the Korsmeyer-Peppas model. The antimicrobial activity of functionalised PAA and multilayered CUR-loaded hydrogel microcarriers with quaternary ammonium function was assessed against Staphylococcus aureus and Serratia marcescens by the agar diffusion assay method. Only a limited inhibition zone of PAA was observed, but in the case of both antimicrobial decorated PAA and the corresponding multilayered nanocarriers, the inhibitory activity increase was achieved against both strains of bacteria.

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“…The synthesis of gold nanoparticles was conducted based on the modified procedure by Frens. , For this, 25 mL of a 0.01% (w/v) HAuCl 4 solution in water was heated to 100 °C and then mixed with 180 μL of an aqueous sodium citrate solution (1% [w/v]). The color of the solution changed gradually from faint yellow to red.…”

Section: Methodsmentioning

confidence: 99%

Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air–Blood Barrier Crossing

Taschauer

Polzer

Pöschl

et al. 2020

ACS Appl. Mater. Interfaces

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Current nucleic acid (NA) nanotherapeutic approaches face challenges because of shortcomings such as limited control on loading efficiency, complex formulation procedure involving purification steps, low load of NA cargo per nanoparticle, endosomal trapping, and hampered release inside the cell. When combined, these factors significantly limit the amount of biologically active NA delivered per cell in vitro , delivered dosages in vivo for a prolonged biological effect, and the upscalability potential, thereby warranting early consideration in the design and developmental phase. Here, we report a versatile nanotherapeutic platform, termed auropolyplexes, for improved and efficient delivery of small interfering RNA (siRNA). Semitelechelic, thiolated linear polyethylenimine (PEI) was chemisorbed onto gold nanoparticles to endow them with positive charge. A simple two-step complexation method offers tunable loading of siRNA at concentrations relevant for in vivo studies and the flexibility for inclusion of multiple functionalities without any purification steps. SiRNA was electrostatically complexed with these cationic gold nanoparticles and further condensed with polycation or polyethyleneglycol–polycation conjugates. The resulting auropolyplexes ensured complete complexation of siRNA into nanoparticles with a high load of ∼15,500 siRNA molecules/nanoparticle. After efficient internalization into the tumor cell, an 80% knockdown of the luciferase reporter gene was achieved. Auropolyplexes were applied intratracheally in Balb/c mice for pulmonary delivery, and their biodistribution were studied spatio-temporally and quantitatively by optical tomography. Auropolyplexes were well tolerated with ∼25% of the siRNA dose remaining in the lungs after 24 h. Importantly, siRNA was released from auropolyplexes in vivo and a fraction also crossed the air–blood barrier, which was then excreted via kidneys, whereas >97% of gold nanoparticles were retained in the lung. Linear PEI-based auropolyplexes offer a combination of successful endosomal escape and better biocompatibility profile in vivo . Taken together, combined chemisorption and complexation endow auropolyplexes with crucial biophysical attributes, enabling a versatile and upscalable nanogold-based platform for siRNA delivery in vitro and in vivo .

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Layer-by-Layer Assembled Gold Nanoparticles for the Delivery of Nucleic Acids

Cited by 11 publications

References 24 publications

Oligolayer-Coated Nanoparticles: Impact of Surface Topography at the Nanobio Interface

Oligolayer-Coated Nanoparticles: Impact of Surface Topography at the Nanobio Interface

Multilayered Curcumin-Loaded Hydrogel Microcarriers with Antimicrobial Function

Combined Chemisorption and Complexation Generate siRNA Nanocarriers with Biophysics Optimized for Efficient Gene Knockdown and Air–Blood Barrier Crossing

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