Layer-by-Layer Assembled Gold Nanoparticles for siRNA Delivery

Elbakry, Asmaa M.; Zaky, Alaa A.; Liebl, Renate; Rachel, Reinhard; Goepferich, Achim; Breunig, Miriam

doi:10.1021/nl9003865

Cited by 397 publications

(408 citation statements)

References 39 publications

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“…Self-assembled layerby-layer (LbL) system namely PEI/siRNA/PEI-AuNPs was developed utilizing mercaptoundecanoic acid (MUA). The delivery system reduced the EGFP production to about 28% in CHO-K1 cells (Elbakry et al, 2009). The high stability of LbLcoated AuNPs retarded the release of siRNA in cytoplasm.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Polymers in Small-Interfering RNA Delivery

Singha

Namgung

Kim

2011

Nucleic Acid Therapeutics

181

112

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This review will cover the current strategies that are being adopted to efficiently deliver small interfering RNA using nonviral vectors, including the use of polymers such as polyethylenimine, poly(lactic-co-glycolic acid), polypeptides, chitosan, cyclodextrin, dendrimers, and polymers-containing different nanoparticles. The article will provide a brief and concise account of underlying principle of these polymeric vectors and their structural and functional modifications which were intended to serve different purposes to affect efficient therapeutic outcome of small-interfering RNA delivery. The modifications of these polymeric vectors will be discussed with reference to stimuli-responsiveness, target specific delivery, and incorporation of nanoconstructs such as carbon nanotubes, gold nanoparticles, and silica nanoparticles. The emergence of small-interfering RNA as the potential therapeutic agent and its mode of action will also be mentioned in a nutshell.

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Section: Gold Nanoparticlesmentioning

confidence: 99%

Polymers in Small-Interfering RNA Delivery

Singha

Namgung

Kim

2011

Nucleic Acid Therapeutics

181

112

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“…LbL assembly allows for the construction of hierarchical and multifunctional polyelectrolyte multilayers on a charged colloidal core substrate (13)(14)(15), and it is possible to incorporate therapeutics such as siRNA, inhibitors, or proteins in the multilayers around the core nanoparticle (NP) (16). The LbL platform provides improved biocompatibility that reduces off-target toxicity of the delivered payloads (17), and the LbL stealth coatings provide extended blood plasma half-life when applied to liposomes, QDs, gold, and other NP systems (15,18).…”

mentioning

confidence: 99%

Layer-by-layer assembled fluorescent probes in the second near-infrared window for systemic delivery and detection of ovarian cancer

Dang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

168

133

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Fluorescence imaging in the second near-infrared window (NIR-II, 1,000-1,700 nm) features deep tissue penetration, reduced tissue scattering, and diminishing tissue autofluorescence. Here, NIR-II fluorescent probes, including down-conversion nanoparticles, quantum dots, single-walled carbon nanotubes, and organic dyes, are constructed into biocompatible nanoparticles using the layer-bylayer (LbL) platform due to its modular and versatile nature. The LbL platform has previously been demonstrated to enable incorporation of diagnostic agents, drugs, and nucleic acids such as siRNA while providing enhanced blood plasma half-life and tumor targeting. This work carries out head-to-head comparisons of currently available NIR-II probes with identical LbL coatings with regard to their biodistribution, pharmacokinetics, and toxicities. Overall, rare-earth-based down-conversion nanoparticles demonstrate optimal biological and optical performance and are evaluated as a diagnostic probe for high-grade serous ovarian cancer, typically diagnosed at late stage. Successful detection of orthotopic ovarian tumors is achieved by in vivo NIR-II imaging and confirmed by ex vivo microscopic imaging. Collectively, these results indicate that LbL-based NIR-II probes can serve as a promising theranostic platform to effectively and noninvasively monitor the progression and treatment of serous ovarian cancer.second near-infrared window | layer by layer | systemic comparison | ovarian tumor detection | deep penetration

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“…The technique employs a NIR light trigger to facilitate site specific silencing through biomolecule delivery and the conversion of electronic energy to heat [245][246][247]. Nanocarriers typically target proteins, peptides, nucleic acids, DNA, and RNA [71,241,[248][249][250][251][252][253]. Polymeric structures and liposomes are usually employed as carriers, however, as stated above; polymers are large and cytotoxic and liposomes prone to leakage [254][255][256].…”

Section: Photothermal Transfectionmentioning

confidence: 99%

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

2016

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By electrodeposition and galvanic replacement reaction, we developed a facile, time-saving, cost-effective, and environmentally friendly, two-step synthesis route to obtain a controllable cobalt oxide/Au hierarchically nanostructured electrode for glucose sensing. The nanomaterials were characterized by transmission electron microscopy, scanning electron microscopy, Raman spectroscopy, energy-dispersive spectrometry, and X-ray photoelectron spectroscopy, meanwhile, the sensing performance was investigated by cyclic voltammetry and amperometric response. The results revealed that this novel electrode exhibited excellent electrocatalytic performance toward glucose oxidation, with a wide double-linear range from 0.2 μM to 20 mM and a low detection limit of 0.1 μM based on a signal-to-noise ratio of 3, which was mainly attributed to the ability of loading a small amount of Au with good electron conductivity on the surface of cobalt oxide nanosheets with large active surface area and synergistic electrocatalytic activity of Au and cobalt oxide toward glucose electrooxidation. This facile, sensitive, and selective glucose sensor is also proven to be suitable for the detection of glucose in human serum.

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Layer-by-Layer Assembled Gold Nanoparticles for siRNA Delivery

Cited by 397 publications

References 39 publications

Polymers in Small-Interfering RNA Delivery

Polymers in Small-Interfering RNA Delivery

Layer-by-layer assembled fluorescent probes in the second near-infrared window for systemic delivery and detection of ovarian cancer

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

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