Layer-by-Layer Assembled Enzyme Cascade Bioanode for Catalyzing Oxidation of Sucrose

Zhang, Yuanyuan; Arugula, Mary A.; Williams, Shannon Tierney; Simonian, Aleksandr

doi:10.1149/06636.0009ecst

Cited by 3 publications

(2 citation statements)

References 11 publications

(20 reference statements)

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“…The maximum power density from MG and [Ni(phendion)(phen) 2 ]Cl 2 were 209 ± 3 μW/cm 2 and 405 ± 6 μW/cm 2 on GCE succeeding most of other reported disaccharides biofuel cells in literature. 98,99 The Table I shows some of the examples of single, bi and cascade enzyme electrodes constructed for biofuel cell applications using different immobilization strategies and Lbl.…”

Section: Other Lbl/cnt Based Enzyme Biosensorsmentioning

confidence: 99%

Review—Nanocarbon-Based Multi-Functional Biointerfaces: Design and Applications

Arugula

Simonian

2016

ECS J. Solid State Sci. Technol.

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Nanocarbons are promising materials in a growing list of applications due to their unique intrinsic and potentially tunable properties. Carbon nanotubes are one of the most favorable nanocarbons used in biosensors R&D due to their excellent structural and electronic properties. Scaffolding of different functionally active biopolymers on CNTs and their further layer-by-layer (LbL) assembly on the appropriate interfaces allow creation of new insights and concepts for the development of multiple novel technological biointerfaces based on intercalation of CNTs covered with different oppositely charged biopolymers and enzymes. We concentrate on how different enzymes assembled at the same interface by the LbL technique can operate in different modes in the construction of novel bioelectronic applications. In this review we focus on the design of unique multifunctional devices for biomedical, environment and energy applications. This paper reviews the research results of the last several years and summarizes the carbon nanotubes application in biosensors.The nanocarbon family, made of dimensionally confined sp 2 bonded carbonaceous materials such as carbon nanotubes (CNTs), graphene and fullerenes is a growing subject of interest when designing next generation functional materials for quantitative and qualitative detection of chemical and biological moieties as well as implications for medicine, environment and energy. 1-4 New materials and fabrication strategies are continuously created to meet the needs of growing technology in chemical sensing and biosensing. CNTs and graphene hold a great promise for such applications due to their high surface area (greater interaction zone), electrical properties (fast electron transfer), mechanical properties and greater modulation properties with analytes. Although several other nanomaterials have excellent functions, CNTs are the leading choice, although superseded by graphene. The review mostly focuses on CNTs, which were a technological breakthrough in 1991, and since have been well studied and widely recognized nanomaterials for their excellent and unique physico-chemical properties such as good electrical conductivity, high stability and strong adsorptive ability. 5-8 These materials continue to be fascinating and important factor for current and future technologies especially in biosensor technology.It is well known that the sensors have historically been made with pristine and functionalized CNTs. Nevertheless, selectivity is an important parameter and could be low due to lack of interaction between the analyte and CNTs. Therefore, an emerging group of hybrid structure derived nanomaterials, modified with sensing element that can offer an attractive combination of intrinsic physical and chemical properties for a wide range of applications including mimetic biominerals, biomedicine and biosensors is crucial. 9-11 The sensing elements can range from synthetic molecules to biomolecules. The integration of CNTs with a wide variety of biological molecules such as biopolymers, b...

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Section: Other Lbl/cnt Based Enzyme Biosensorsmentioning

confidence: 99%

Review—Nanocarbon-Based Multi-Functional Biointerfaces: Design and Applications

Arugula

Simonian

2016

ECS J. Solid State Sci. Technol.

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“…Layer-by-layer (LbL) assembly involving consecutive assembly of oppositely charged species has become a prime choice for fabrication of thin film nanostructures (1). In our previous studies, LbL assembly showed great feasibility as a simple and efficient way to construct multi-functional biointerfaces with tailored nano-architecture in development of discriminative biosensor (2,3), and biofuel cell (4,5). It demonstrated striking advantages in precise control of composition, thickness of film, wide selection of biomaterials, and a mild microenvironment to keep their functionality (6).…”

Section: Introductionmentioning

confidence: 99%

Temperature-Responsive PNIPAM-g-alginate Gel Wrapped with LbL Assembled Chitosan/DNA Membranes for Development of Controlled Drug Release System

et al. 2016

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While considerable consumers experience more-than-recommended-dose usage of commercially available scar cream for scar therapy treatment, we report here a novel and smart thermally controlled drug delivery gel patch system for scar therapy purpose. The gel patch contains a core of amine-terminated-poly(N-isopropylacrylamide) (NH2-PNIPAM) grafted alginate wrapped with outer membranes consisting of layer-by-layer (LbL) assembled chitosan and DNA thin films. The outer membrane via LbL assembly controls the rate and profile of model dye rhodamine B. A dramatically reduced burst effect in the delivery process and a diffusion controlled behavior was observed for the proposed system. NH2-PNIPAM cross-linked onto side chain of alginate exhibits contraction and expansion above and below its lower critical solution temperature (LCST: 36℃) and therefore serves as a switch turning “on and off” the release of drug entrapped in the core. The difference in the release of Texas-red conjugated epidermal growth factor (EGF-TR) at 36℃ and 22℃ were monitored with fluorescence microscopy and the system showed higher release of EGF-TR at 36℃ than at 22 ◦C. Below LCST (at 22℃), the expanded NH2-PNIPAM resembles stretched arms that close the release of EGF-TR, while above LCST (at 36℃), the NH2-PNIPAM arms coil and allow the release of EGF, showing great potential for application in controlled drug release system.

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