Layer-by-layer adsorption of biopolyelectrolytes as a universal approach to fabrication of protein-loaded microparticles

Abstract

This study was aimed at examination of microparticles formed via the layer by layer adsorption of dextran sulfate and chitosan onto the insoluble complexes of various proteins with polyanions. Microparticles with all tested proteins were stable at pH values of 1-5. At pH > 6 the mucoadhesivity of the microparticles changed and the encapsulated proteins were released. Microparticles were able to protect the proteins from proteases. Proteinous protease inhibitors encapsulated as well (2-3%) completely prevented protein proteol ysis. The pharmacological effect of microencapsulated insulin was studied in vivo using the model of chronic diabetes in rats, which were treated by oral administration.

Polyelectrolyte micro- and nanoparticles with doxorubicin

“…The layer by layer adsorption of oppositely charged polyelectrolytes on colloidal particles of dif ferent compositions and structures is widely used for encapsulating biologically active substances (BASs) [6][7][8][9]. This immobilization method has the following advantages: the process can be performed in aqueous media under mild conditions and can be used to pro duce preparations of various structures that corre spond to their intended use.…”

Pharmacopoeial Methods for Determining Particle Size. Report 2. Quality Control of Peptide and Protein Based Suspensions for Subcutaneous Administration Drugs

“…Развитие физико-химических методов анализа существенно расширяет возможности стандартиза-ции и контроля качества лекарственных препаратов белковой природы [1]. Многочисленные исследования направлены на разработку биосовместимых микрокапсулированных препаратов инсулина, обладающих защит-ной функцией и способных контролируемо высвобождать АФИ.…”

Protein‐Containing Multilayer Capsules by Templating on Mesoporous CaCO3 Particles: POST‐ and PRE‐Loading Approaches

“…Encapsulation of proteins into polymer microcapsules by means of consecutive polymer adsorption onto decomposable matrices offers a way to assemble protein‐containing microparticles with controlled protein release governed by properties of polymers as components of multilayer shell and a number of deposited layers . Such microcapsules have found a number of attractive biologically relevant applications .…”