Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane

Amaro, Mariana; Reina, Francesco; Hof, Martin; Eggeling, Christian; Sezgin, Erdinç

doi:10.1088/1361-6463/aa5dbc

Cited by 123 publications

(144 citation statements)

References 54 publications

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“…The vast majority of membrane order dyes are solvatochromic and display shifts in their emission maxima when found in polar vs . non-polar solvents [214–218]. For example, in Laurdan’s case, the less-polar membrane environment of the ordered phase (often regarded as synonymous with lipid rafts) induces a 50-nm blue shift in the emission maxima.…”

Section: Dysregulation Of Plasma Membrane Biochemical and Biophysicalmentioning

confidence: 99%

Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents

Erazo-Oliveras

Fuentes

Wright

et al. 2018

Cancer Metastasis Rev

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The cell plasma membrane serves as a nexus integrating extra- and intracellular components, which together enable many of the fundamental cellular signaling processes that sustain life. In order to perform this key function, plasma membrane components assemble into well-defined domains exhibiting distinct biochemical and biophysical properties that modulate various signaling events. Dysregulation of these highly dynamic membrane domains can promote oncogenic signaling. Recently, it has been demonstrated that select membrane-targeted dietary bioactives (MTDBs) have the ability to remodel plasma membrane domains and subsequently reduce cancer risk. In this review, we focus on the importance of plasma membrane domain structural and signaling functionalities as well as how loss of membrane homeostasis can drive aberrant signaling. Additionally, we discuss the intricacies associated with the investigation of these membrane domain features and their associations with cancer biology. Lastly, we describe the current literature focusing on MTDBs, including mechanisms of chemoprevention and therapeutics in order to establish a functional link between these membrane-altering biomolecules, tuning of plasma membrane hierarchal organization, and their implications in cancer prevention.

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Section: Dysregulation Of Plasma Membrane Biochemical and Biophysicalmentioning

confidence: 99%

Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents

Erazo-Oliveras

Fuentes

Wright

et al. 2018

Cancer Metastasis Rev

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“…This provides a more representative measure of plasma membrane organization. In addition, although laurdan and Di4 are both used to quantify membrane order[151], recent findings highlight the fact that they probe different properties of the membrane, with Di4 being more sensitive to cholesterol status[152]. Di4 excites at 488 nm and its emission maximum emits at 565 nm or 605 nm in ordered and disordered membranes, respectively[149,151].…”

Section: Introductionmentioning

confidence: 99%

Emerging role of chemoprotective agents in the dynamic shaping of plasma membrane organization

Fuentes

Salinas

Kim

et al. 2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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In the context of an organism, epithelial cells by nature are designed to be the defining barrier between self and the outside world. This is especially true for the epithelial cells that form the lining of the digestive tract, which absorb nutrients and serve as a barrier against harmful substances. These cells are constantly bathed by a complex mixture of endogenous (bile acids, mucus, microbial metabolites) and exogenous (food, nutrients, drugs) bioactive compounds. From a cell biology perspective, this type of exposure would directly impact the plasma membrane, which consists of a myriad of complex lipids and proteins. The plasma membrane not only functions as a barrier but also as the medium in which cellular signaling complexes form and function. This property is mediated by the organization of the plasma membrane, which is exquisitely temporally (nanoseconds to minutes) and spatially (nanometers to micrometers) regulated. Since numerous bioactive compounds found in the intestinal lumen can directly interact with lipid membranes, we hypothesize that the dynamic reshaping of plasma membrane organization underlies the chemoprotective effect of select membrane targeted dietary bioactives (MTDBs).

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confidence: 97%

“…The original paper of di-4-ANEPPDHQ paper (Jin et al , 2006 4 ) as well as Sezgin et al , 2014 2 show the progressive shift of these dyes. And a recent report by Amaro et al , 2017 5 shows the complicated spectral shift of ANEP dye unlike a robust response of Laurdan to molecular order.“C-laurdan, a probe first synthesised and characterised in 2007 was derived from laurdan by adding a carboxyl group to the polar head, and labels the PM of eukaryotic cells more effectively than laurdan.” Here the original C-Laurdan should be cited (Kim et al , 2007) 6 From the Materials and Methods, I could not get what is the excitation wavelength of NileRed?Authors should comment on whether Fraction Mapper can read any spectral imaging file? (lsm, czi, lif etc)…”

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confidence: 99%

Using spectral decomposition of the signals from laurdan-derived probes to evaluate the physical state of membranes in live cells

2017

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We wanted to investigate the physical state of biological Background: membranes in live cells under the most physiological conditions possible.For this we have been using laurdan, C-laurdan or M-laurdan to label Methods: a variety of cells, and a biphoton microscope equipped with both a thermostatic chamber and a spectral analyser. We also used a flow cytometer to quantify the 450/530 nm ratio of fluorescence emissions by whole cells.We find that using all the information provided by spectral analysis to Results: perform spectral decomposition dramatically improves the imaging resolution compared to using just two channels, as commonly used to calculate generalized polarisation (GP). Coupled to a new plugin called Fraction Mapper, developed to represent the fraction of light intensity in the first component in a stack of two images, we obtain very clear pictures of both the intra-cellular distribution of the probes, and the polarity of the cellular environments where the lipid probes are localised. Our results lead us to conclude that, in live cells kept at 37°C, laurdan, and M-laurdan to a lesser extent, have a strong tendency to accumulate in the very apolar environment of intra-cytoplasmic lipid droplets, but label the plasma membrane (PM) of mammalian cells ineffectively. On the other hand, C-laurdan labels the PM very quickly and effectively, and does not detectably accumulate in lipid droplets.From using these probes on a variety of mammalian cell lines, as Conclusions: well as on cells from and , we conclude Drosophila Dictyostelium discoideum that, apart from the lipid droplets, which are very apolar, probes in intracellular membranes reveal a relatively polar and hydrated environment, suggesting a very marked dominance of liquid disordered states. PMs, on the other hand, are much more apolar, suggesting a strong dominance of liquid ordered state, which fits with their high sterol contents.

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Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane

Cited by 123 publications

References 54 publications

Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents

Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents

Emerging role of chemoprotective agents in the dynamic shaping of plasma membrane organization

Using spectral decomposition of the signals from laurdan-derived probes to evaluate the physical state of membranes in live cells

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