To support development of Martian Ascent Vehicles, analysis tools are needed to support the development of Guidance, Navigation, and Control requirements. This paper presents a focused approach to Navigation analysis to capture development of requirements on initial state knowledge and inertial sensor capabilities. A simulation and analysis framework was used to assess the capability of a range of sensors to operate inertially along a range of launch trajectories. The baseline Martian Ascent Vehicle was used as the input for optimizing a set of trajectories from each launch site. These trajectories were used to perform Monte Carlo analysis dispersing error sensor terms and their effects on integrated vehicle performance. Additionally, this paper provides insight into the use of optical navigation techniques to assess state determination and the potential to use observations of local extraplanetary bodies to estimate state. This paper provides an initial level of performance assessment of navigation components to support continued requirements development of a Martian Ascent Vehicle with applications to both crew and sample return missions.