Latitudinal Distribution of Ammonia-Oxidizing Bacteria and Archaea in the Agricultural Soils of Eastern China

Jiang, Hongchen; Huang, Liuqin; Deng, Ye; Wang, Shang; Zhou, Yu; Liu, Li; Dong, Hailiang

doi:10.1128/aem.01617-14

Cited by 51 publications

(37 citation statements)

References 67 publications

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“…Most of AOA amoA gene sequences were equally distributed into two Nitrososphaera clades (together 88.1% of total sequences). This pattern of low richness of AOA is a common observation when soils are sampled at a single location (Jiang et al, 2014a).…”

Section: Aoa and Aob Abundance And Community Composition Response To mentioning

confidence: 69%

Ammonia-oxidizing bacteria are more responsive than archaea to nitrogen source in an agricultural soil

Ouyang

Norton

Stark

et al. 2016

Soil Biology and Biochemistry

238

113

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In the majority of agricultural soils, ammonium (NH +) is rapidly converted to nitrate (NO 3-) in the biological ammonia and nitrite oxidation processes known as nitrification. The often rate-limiting step of ammonia oxidation to nitrite is mediated by ammonia oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA). The response of AOA and AOB communities to organic and conventional nitrogen (N) fertilizers, and their relative contributions to the nitrification process were examined for an agricultural silage corn system using a randomized block design with 4 N treatments: control (no additional N), ammonium sulfate (AS) fertilizer at 100 and 200 kg N ha-1 , and steer-waste compost (200 kg total N ha-1) over four seasons. DNA was extracted from the soil, and real-time PCR and 454-pyrosequencing were used to evaluate the quantity and diversity of the amoA gene which encodes subunit A of ammonia monooxygenase. Soil pH, nitrate pools, and nitrification potentials were influenced by ammonium and organic fertilizers after the first fertilization, while changes in AOB abundance and community structure were not apparent until after the second fertilization or later. The abundance of AOA was always greater than AOB but was unaffected by N treatments. In contrast, AOB abundance and community structure were changed significantly by ammonium fertilizers. Specific inhibitors of nitrification were used to evaluate the relative contribution of AOA and AOB to nitrification. We found that AOB dominantly contributed to potential nitrification activity determined at 1 mM ammonium in soil slurries and nitrification potential activity was higher in soils treated with ammonium fertilizers relative to control soils. However, AOA dominated gross nitrification activity in moist soils. Our result suggests that AOB activity and community are more responsive to ammonium fertilizers than AOA but that in situ nitrification rate is controlled by ammonium availability in this agricultural soil. Understanding this response of AOA and AOB to N fertilizers may contribute to improving strategies for the management of nitrate production in agricultural soils.

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Section: Aoa and Aob Abundance And Community Composition Response To mentioning

confidence: 69%

Ammonia-oxidizing bacteria are more responsive than archaea to nitrogen source in an agricultural soil

Ouyang

Norton

Stark

et al. 2016

Soil Biology and Biochemistry

238

113

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“…The ABT analysis can give relative influence of individual environmental parameters on MCC, but it is limited to quantitatively assess how much MCC variations can be separately explained by environmental factors and spatial factors. In order to quantify the relative importance of environmental and spatial factors in shaping microbial community, a canonical correspondence analysis (CCA)-based variation partitioning analysis (VPA) was carried out according to the methods described previously (Jiang et al, 2014). Briefly, a set of spatial variables were firstly produced through the method of principal coordinates of neighbor matrices (PCNM) analysis according to the longitude and latitude coordinates of sampling sites (Borcard and Legendre, 2002).…”

Section: Methodsmentioning

confidence: 99%

Distinct Factors Shape Aquatic and Sedimentary Microbial Community Structures in the Lakes of Western China

Yang

Jiang

et al. 2016

Front. Microbiol.

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Little is known about the relative importance of spatial and environmental factors to structuring aquatic and sedimentary microbial biogeography in lakes. Here, we investigated the microbial community composition (MCC) of the water (n = 35) and sediment (n = 35) samples from 16 lakes in western China (salinity: freshwater to salt saturation; pairwise geographical distance: 9–2027 km) using high-throughput sequencing and evaluated the relative importance of spatial and environmental factors to microbial (including total, abundant, and rare) distributions. Our results showed that spatial factors were more important than environmental factors in shaping the biogeography of aquatic and sedimentary microbial communities in the studied lakes, and spatial factors on abundant microbial community was stronger than that on the total/rare microbial communities. Moreover, sedimentary rare MCC might be more sensitive to environmental factors than its aquatic counterpart. Such different biogeography responses of total, abundant, and rare communities to environmental and spatial factors could be ascribed to different physiochemical properties between water and sediment. Collectively, this study expands our understanding of factors shaping microbial biogeography of total, abundant, and rare communities between waters and sediments of lakes.

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“…In many cases, the Nitrososphaera cluster is dominant in soils worldwide (Pester et al 2012;Jiang et al 2014). The AOA at our study site consisted exclusively of the Nitrososphaera cluster.…”

Section: Characteristics Of the Identified Ammonia Oxidizersmentioning

confidence: 99%

Characteristics of ammonia oxidation potentials and ammonia oxidizers in mineral soil under Salix polaris–moss vegetation in Ny-Ålesund, Svalbard

Hayashi

Shimomura²,

Morimoto

et al. 2015

Polar Biol

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Although nitrification is a unique and important process in the nitrogen cycle with respect to ammonium consumption and nitrate production, limited information on this process is available for high-Arctic soils. We elucidated the ammonia oxidation potentials (AOPs) and characteristics of ammonia-oxidizing bacteria (AOB) and archaea (AOA) in mineral soils under climax vegetation, i.e., Salix polaris (polar willow)-moss vegetation, on a coastal hill in Ny-Å lesund, Svalbard. AOPs at 10°C were determined by incubation with sufficient substrate (2 mM ammonium). The ammonia monooxygenase subunit A (amoA) genes of AOB and AOA were analyzed by using quantitative polymerase chain reaction and pyrosequencing. AOPs ranged from 1.1 to 14.1 ng N g -1 dry soil h -1 -relatively low but of a similar order to the gross nitrification rates reported in another Svalbard study. AOP was positively correlated with thickness of the moss layer (P \ 0.01), soil water content, and ammonium nitrogen content (P \ 0.05). The population sizes of both AOB and AOA were not significantly related to AOP or edaphic factors. For AOB-amoA, six major operational taxonomic units (OTUs) were identified, all of which were classified into the Nitrosospira Mount Everest cluster. For AOAamoA, six major OTUs were also identified, five of which were grouped with sequences from cold environments within clade A of the Nitrososphaera cluster, i.e., species known to have low, or no, AOP. It is, therefore, possible that the AOPs measured at the study site were driven mainly by psychrotolerant AOB.

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Latitudinal Distribution of Ammonia-Oxidizing Bacteria and Archaea in the Agricultural Soils of Eastern China

Cited by 51 publications

References 67 publications

Ammonia-oxidizing bacteria are more responsive than archaea to nitrogen source in an agricultural soil

Ammonia-oxidizing bacteria are more responsive than archaea to nitrogen source in an agricultural soil

Distinct Factors Shape Aquatic and Sedimentary Microbial Community Structures in the Lakes of Western China

Characteristics of ammonia oxidation potentials and ammonia oxidizers in mineral soil under Salix polaris–moss vegetation in Ny-Ålesund, Svalbard

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