Latex Immunoagglutination Assays

Molina-Bolı́var, J.A.; Galisteo‐González, F.

doi:10.1081/mc-200045819

Cited by 64 publications

(61 citation statements)

References 156 publications

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“…In this case, latex particles are coated with immunoglobulin (Ig) to create larger particles during the course of the immunological reaction, whereby the intensity of the scattered light is increased and the detection limit can be reduced 1000-fold. [41] This in turn makes it possible to determine very low analyte concentrations, for example, IgA and IgM in cerebrospinal fluids.…”

Section: Immunonephelometrymentioning

confidence: 99%

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Clinical Chemistry: Challenges for Analytical Chemistry and the Nanosciences from Medicine

Durner

2010

Angew Chem Int Ed

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Clinical chemistry and laboratory medicine can look back over more than 150 years of eventful history. The subject encompasses all the medicinal disciplines as well as the remaining natural sciences. Clinical chemistry demonstrates how new insights from basic research in biochemical, biological, analytical chemical, engineering, and information technology can be transferred into the daily routine of medicine to improve diagnosis, therapeutic monitoring, and prevention. This Review begins with a presentation of the development of clinical chemistry. Individual steps between the drawing of blood and interpretation of laboratory data are then illustrated; here not only are pitfalls described, but so are quality control systems. The introduction of new methods and trends into medicinal analysis is explored, along with opportunities and problems associated with personalized medicine.

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Section: Immunonephelometrymentioning

confidence: 99%

“…[28] LFAs are extentions of the homogeneous latex agglutination test introduced in 1956. [40][41][42] These are based on the same principle as immunological assays, namely an antigen-antibody reaction. They have, in addition, chromatographic characteristics.…”

Section: Basic Considerations and General Remarksmentioning

confidence: 99%

Clinical Chemistry: Challenges for Analytical Chemistry and the Nanosciences from Medicine

Durner

2010

Angew Chem Int Ed

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“…Because of many advantages over other tests, that is, fast, easy, highly selective, and inexpensive, LAT is applied to detect over 100 infectious diseases (38)(39)(40).…”

Section: Latex Agglutination Testmentioning

confidence: 99%

“…This method is applicable to the detection of polyvalent antigen such as proteins and microorganisms (38). The most familiar application of this method is the pregnancy test in which human chorionic gonadotropin (HCG) protein in the blood and urine is detected by polystyrene (PS) latex coated with monoclonal antibody leading to the visible agglutination within 3 min (42,43).…”

Section: Direct Latex Agglutination Testmentioning

confidence: 99%

“…3. 6. This approach is applicable to mono and polyvalent antigens, that is, drugs, hormones, and proteins (38). Currently, many infectious diseases including leptospirosis, amebiasis, and Entamoeba histolytica Cyst Passage can be diagnosed with high sensitivity and specificity (>90%) by using the indirect LAT (32,34,44).…”

Section: Indirect Latex Agglutination Testmentioning

confidence: 99%

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Evolution in Malaria Disease Detection: From Parasite Visualization to Colloidal‐Based Rapid Diagnostic

Polpanich

Tangboriboonrat

Elaı̈ssari

2007

Colloidal Nanoparticles in Biotechnology

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Trends in Immunoassay Techniques

Gómez‐Hens

Aguilar-Caballos

2010

Encyclopedia of Analytical Chemistry

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This article attempts to provide an overview of different approaches proposed to improve the performance of immunoassay techniques, most of which are used in routine clinical analysis and are finding wide acceptance in other analytical areas, such as environmental and food analysis. The term immunoassay here encompasses all the methods that involve the formation of antigen‐antibody complex to attain the separation and/or detection of analytes, including conventional immunoassays, immunosensors, and immunoaffinity techniques. Advances in the production of antibodies, in the availability of new labels, and in the immobilization of immunoreagents, which are allowing the improvement of the selectivity, sensitivity and robustness of these assays, are described. The recent use of different nanomaterials as labels, nanoscaffolds, and separation media in immunoassay techniques is especially emphasized. Also, the development of new approaches to increase the usefulness of immunoassays for multianalyte detection is shown through the different microarray systems proposed. The efforts carried out to miniaturize immunoassays are described by discussing the usefulness of microfluidic systems. Finally, recent achievements in immunoextraction, alone or in combination with other separation techniques, such as liquid chromatography and capillary electrophoresis, are also included.

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Latex Immunoagglutination Assays

Cited by 64 publications

References 156 publications

Clinical Chemistry: Challenges for Analytical Chemistry and the Nanosciences from Medicine

Clinical Chemistry: Challenges for Analytical Chemistry and the Nanosciences from Medicine

Evolution in Malaria Disease Detection: From Parasite Visualization to Colloidal‐Based Rapid Diagnostic

Trends in Immunoassay Techniques

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