Lateral shearing digital holographic imaging of small biological specimens

Singh, Amardeep; Anand, Arun; Leitgeb, Rainer A.; Javidi, Bahram

doi:10.1364/oe.20.023617

Cited by 151 publications

(60 citation statements)

References 17 publications

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“…For dynamic imaging of cells, microscope setups with sub-nanometer temporal stability is required. Such microscopes could be implemented with common path self-referencing geometry and can image sub-nanometer oscillations of cell membranes [8][9][10]. The next step is to combine the static as well as dynamic parameters provided by this type of digital holographic microscopes for identification of cells.…”

Section: Discussionmentioning

confidence: 99%

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Identification of malaria infected red blood samples by digital holographic quantitative phase microscope

Patel

Chhaniwal

Javidi³

et al. 2015

SPIE Proceedings

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Development of devices for automatic identification of diseases is desired especially in developing countries. In the case of malaria, even today the gold standard is the inspection of chemically treated blood smears through a microscope. This requires a trained technician/microscopist to identify the cells in the field of view, with which the labeling chemicals gets attached. Bright field microscopes provide only low contrast 2D images of red blood cells and cell thickness distribution cannot be obtained. Quantitative phase contrast microscopes can provide both intensity and phase profiles of the cells under study. The phase information can be used to determine thickness profile of the cell. Since cell morphology is available, many parameters pertaining to the 3D shape of the cell can be computed. These parameters in turn could be used to decide about the state of health of the cell leading to disease diagnosis. Here the investigations done on digital holographic microscope, which provides quantitative phase images, for comparison of parameters obtained from the 3D shape profile of objects leading to identification of diseased samples is described.

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Section: Discussionmentioning

confidence: 99%

“…Digital holographic microscopy is such a technique, providing a means for effective three-dimensional imaging of micro-objects [1][2][3][4][5][6][7][8][9][10]. Digital holograms directly provide the phase information of the object, from which its thickness profile can be reconstructed [6,7].…”

Section: Introductionmentioning

confidence: 99%

Identification of malaria infected red blood samples by digital holographic quantitative phase microscope

Patel

Chhaniwal

Javidi³

et al. 2015

SPIE Proceedings

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“…This technique has been proposed with incoherent illumination [4][5][6][7][8][9][10] to create speckle free images; however, the compactness of the imager is then compromised since a rather large distance (several centimeters) is needed between the source and the sample to obtain enough spatial coherence. Other compact common-path interferometric methods have been investigated to obtain quantitative phase imaging based on lateral phase shifting [11,12].…”

Section: Introductionmentioning

confidence: 99%

Compact lensless phase imager

Rostykus¹,

Soulez²,

Unser³

et al. 2017

Opt. Express

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Lensless quantitative phase imaging is of high interest for obtaining a large field of view (FOV), typically the size of the camera chip, to observe biological cell material with high contrast. It has the potential to be widely spread due to its inherent simplicity. However, the tradeoff is the added complexity due to the illumination. Current illumination systems are several centimeters away from the sample, use mechanics to obtain super resolution (i.e., smaller than the detector pixel size) or different illumination directions, and block the view to the sample. In this paper, we propose and demonstrate a side illumination system which reduces the height by an order of magnitude while providing an unobstructed view of the sample. We achieve this by shaping the illumination using multiplexed analog holograms that produce 9 illumination angles. We demonstrate experimentally imaging of phase samples with a FOV of ~17mm 2 .

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“…The intrinsic optical parameters of the human RBC, such as absorption coefficient, scattering coefficient, anisotropy factor and hence complex refractive index (RI) were determined both experimentally and theoretically by Friebel et al [11]. The other optical techniques at the individual cell level are; interferometric quantitative phase-microscopy [12,13], digital holographic phase-contrast and differential interference ISSN: 1226-4776(Print) / ISSN: 2093-6885(Online) DOI: http://dx.doi.org/10.3807/JOSK.2016.20.1.078 contrast microscopy [14][15][16][17][18], lateral shearing digital holographic imaging [19], Fourier-phase microscopy [20], Hilbert phasemicroscopy (HPM) [21,22], diffraction phase microscopy and tomographic phase-microscopy [3,[23][24][25][26]. All have enabled the extraction of sub-nanometer path-length changes, 3D RI map and millisecond scale fluctuation dynamics of RBC membrane to enhance the understanding of RBCs significantly [27].…”

Section: Introductionmentioning

confidence: 99%

Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging

Kumar

Srivastava

Mehta

et al. 2016

Journal of the Optical Society of Korea

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Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationary in interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innate morphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laser beam is an alternative modality to overcome contact-induced perturbation but at the same time a steeply focused laser beam might cause photo-damage. In order to address both the photo-damage and substrate adherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beam of 'arbitrary intensity profile' generated by a source having cavity imperfections per se. Thus the immobilized RBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robust Michelson interferometer which was designed from a cubic beam splitter having one surface coated with reflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferograms from bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limited to work in reflection configuration only because of the availability of an upright microscope. Due to RBC's membrane being poorly reflective for visible wavelengths, quantitative information in the signal is weak and therefore, the quality of experimental results is limited in comparison to results obtained in transmission mode by various holographic techniques reported elsewhere.

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Lateral shearing digital holographic imaging of small biological specimens

Cited by 151 publications

References 17 publications

Identification of malaria infected red blood samples by digital holographic quantitative phase microscope

Identification of malaria infected red blood samples by digital holographic quantitative phase microscope

Compact lensless phase imager

Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging

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