Lateral pressures in biomembranes estimated from the dynamics of fluorescent probes

Fulford, Anthony J. C.; Peel, William E.

doi:10.1016/0005-2736(80)90002-4

Cited by 47 publications

(23 citation statements)

References 13 publications

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“…Steady‐state fluorescence anisotropy is an excellent tool for measuring the fluidity of a system using a fluorescence probe. The most common probe used to measure fluorescence anisotropy is 1,6‐diphenyl‐1,3,5‐hexatriene (DPH), which intercalates between the hydrophobic interior of the aggregates thanks to its rod‐like shape and hydrophobic nature . The higher steady‐state anisotropy ( r ) value indicates the restricted movement of DPH in various aggregated structures .…”

Section: Resultsmentioning

confidence: 99%

Hydrophobic End‐Modulated Amino‐Acid‐Based Neutral Hydrogelators: Structure‐Specific Inclusion of Carbon Nanomaterials

Choudhury

Mandal

Brahmachari

et al. 2016

Chemistry A European J

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Hydrophobic end-modulated l-phenylalanine-containing triethylene glycol monomethyl ether tagged neutral hydrogelators (1-4) are developed. Investigations determine the gelators' structure-dependent inclusion of carbon nanomaterials (CNMs) in the self-assembled fibrillar network (SAFIN). The gelators (1, 3, and 4) can immobilize water and aqueous buffer (pH 3-7) with a minimum gelator concentration of 10-15 mg mL(-1). The hydrophobic parts of the gelators are varied from a long chain (C-16) to an extended aromatic pyrenyl moiety, and their abilities to integrate 1 D and 2 D allotropes of carbon (i.e., single-walled carbon nanotubes (SWNTs) and graphene oxide (GO), respectively) within the gel are investigated. Gelator 1, containing a long alkyl chain (C-16), can include SWNTs, whereas the pyrene-containing 4 can include both SWNTs and GO. Gelator 3 fails to incorporate SWNTs or GO owing to its slow rate of gelation and possibly a mismatch between the aggregated structure and CNMs. The involvement of various forces in self-aggregated gelation and physicochemical changes occurring through CNM inclusion are examined by spectroscopic and microscopic techniques. The distinctive pattern of self-assembly of gelators 1 and 4 through J- and H-type aggregation might facilitate the structure-specific CNM inclusion. Inclusion of SWNTs/GO within the hydrogel matrix results in a reinforcement in mechanical stiffness of the composites compared with that of the native hydrogels.

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Section: Resultsmentioning

confidence: 99%

Hydrophobic End‐Modulated Amino‐Acid‐Based Neutral Hydrogelators: Structure‐Specific Inclusion of Carbon Nanomaterials

Choudhury

Mandal

Brahmachari

et al. 2016

Chemistry A European J

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“…Steady‐state fluorescence anisotropy is a popular tool to understand the microenvironment of the aggregated systems 18. 19 1,6‐Diphenyl‐1,3,5‐hexatriene (DPH) is the most widely used fluorescent probe in determining the existence of diverse aggregated structures including vesicles, bilayers, and others. DPH solubilizes itself in the hydrophobic bilayer region of the vesicle because of its rigid, rodlike structure without any polar group 19.…”

Section: Resultsmentioning

confidence: 99%

Spontaneous Formation of Biocompatible Vesicles in Aqueous Mixtures of Amino Acid‐Based Cationic Surfactants and SDS/SDBS

2010

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The spontaneous formation of vesicles by six amino acid-based cationic surfactants and two anionic surfactants (sodium dodecylbenzene sulfonate (SDBS) and sodium dodecyl sulfate (SDS)) is reported. The head-group structure of the cationic surfactants is minutely altered to understand their effect on vesicle formation. To establish the regulatory role of the aromatic group in self-aggregation, both aliphatic and aromatic side-chain-substituted amino acid-based cationic surfactants are used. The presence of aromaticity in any one of the constituents favors the formation of vesicles by cationic/anionic surfactant mixtures. The formation of vesicles is primarily dependent on the balance between the hydrophobicity and hydrophilicity of both cationic and anionic surfactants. Vesicle formation is characterized by surface tension, fluorescence anisotropy, transmission electron microscopy, dynamic light scattering, and phase diagrams. These vesicles are thermally stable up to 65 °C, determined by temperature-dependent fluorescence anisotropy. According to the MTT assay, these catanionic vesicles are nontoxic to NIH3T3 cells, thus indicating their wider applicability as delivery vehicles to cells. Among the six cationic surfactants examined, tryptophan- and tyrosine-based surfactants have the ability to reduce HAuCl(4) to gold nanoparticles (GNPs), which is utilized to obtain in-situ-synthesized GNPs entrapped in vesicles without the need for any external reducing agent.

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“…It can be used to estimate the free volume available to the probe, which in turn can be related to lateral pressure at the level of the probe [18]. Currently, this relationship between fluorescence anisotropy and lateral pressure profile needs further refinement, especially through molecular dynamics simulations.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence anisotropy of diphenylhexatriene and its cationic Trimethylamino derivative in liquid dipalmitoylphosphatidylcholine liposomes: opposing responses to isoflurane

et al. 2012

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BackgroundThe mechanism of action of volatile general anesthetics has not yet been resolved. In order to identify the effects of isoflurane on the membrane, we measured the steady-state anisotropy of two fluorescent probes that reside at different depths. Incorporation of anesthetic was confirmed by shifting of the main phase transition temperature.ResultsIn liquid crystalline dipalmitoylphosphatidylcholine liposomes, isoflurane (7-25 mM in the bath) increases trimethylammonium-diphenylhexatriene fluorescence anisotropy by ~0.02 units and decreases diphenylhexatriene anisotropy by the same amount.ConclusionsThe anisotropy data suggest that isoflurane decreases non-axial dye mobility in the headgroup region, while increasing it in the tail region. We propose that these results reflect changes in the lateral pressure profile of the membrane.

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Lateral pressures in biomembranes estimated from the dynamics of fluorescent probes

Cited by 47 publications

References 13 publications

Hydrophobic End‐Modulated Amino‐Acid‐Based Neutral Hydrogelators: Structure‐Specific Inclusion of Carbon Nanomaterials

Hydrophobic End‐Modulated Amino‐Acid‐Based Neutral Hydrogelators: Structure‐Specific Inclusion of Carbon Nanomaterials

Spontaneous Formation of Biocompatible Vesicles in Aqueous Mixtures of Amino Acid‐Based Cationic Surfactants and SDS/SDBS

Fluorescence anisotropy of diphenylhexatriene and its cationic Trimethylamino derivative in liquid dipalmitoylphosphatidylcholine liposomes: opposing responses to isoflurane

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