Lateral gene transfer drives metabolic flexibility in the anaerobic methane oxidising archaeal family<i>Methanoperedenaceae</i>

McIlroy, Simon Jon; Leu, Andy O.; Ye, Jun; Parks, Donovan H.; Orphan, Victoria J.; Tyson, Gene W.

doi:10.1101/2020.02.06.936641

Cited by 4 publications

(3 citation statements)

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“…Methanoperedens”, GoM Arc I (also known as AAA), was recently described as an anaerobic ethane degrader, and contains two genera “ Candidatus Argoarchaeum” (15, 21) and the thermophilic “ Candidatus Ethanoperedens”(28). These clades are not specifically considered in the present work as they do not appear to be marine methanotrophs, and have been extensively discussed in a recent study (29).…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea

Chadwick¹,

Skennerton²,

Laso-Pérez

et al. 2021

Preprint

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The anaerobic oxidation of methane coupled to sulfate reduction is a microbially mediated process requiring a syntrophic partnership between anaerobic methanotrophic (ANME) archaea and sulfate reducing bacteria (SRB). Based on genome taxonomy, ANME lineages are polyphyletic within the phylum Halobacterota, none of which have been isolated in pure culture. Here we reconstruct 28 ANME genomes from environmental metagenomes and flow sorted syntrophic consortia. Together with a reanalysis of previously published datasets, these genomes enable a comparative analysis of all marine ANME clades. We review the genomic features which separate ANME from their methanogenic relatives and identify what differentiates ANME clades. Large multiheme cytochromes and bioenergetic complexes predicted to be involved in novel electron bifurcation reactions are well-distributed and conserved in the ANME archaea, while significant variations in the anabolic C1 pathways exists between clades. Our analysis raises the possibility that methylotrophic methanogenesis may have evolved from a methanotrophic ancestor.

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Section: Resultsmentioning

confidence: 99%

Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea

Chadwick¹,

Skennerton²,

Laso-Pérez

et al. 2021

Preprint

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“…It has been suggested that some Ca . Methanoperedens archaea are genetically equipped to use As(V) as an electron acceptor as many of the available genomes encode for arsenate reductase (Arr) (Glodowska et al, 2022; Leu, 2020). However, until now there is a lack of laboratory studies linking the presence of arr genes with actual As(V) reduction and CH 4 oxidation.…”

Section: Resultsmentioning

confidence: 99%

Nitrate leaching and its implication for Fe and As mobility in a Southeast Asian aquifer

Głodowska

Smith

et al. 2022

Preprint

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The drinking water quality of millions of people in South and Southeast Asia is at risk due to arsenic (As) contamination of groundwater and insufficient access to water treatment facilities. Intensive use of nitrogen (N) fertilizer increases the possibility of nitrate (NO3-) leaching into aquifers, yet very little is known about how the N cycle will interact with and affect the iron (Fe) and As mobility in aquifers. We hypothesized that input of NO3- into highly methanogenic aquifers can stimulate nitrate-dependent anaerobic methane oxidation (N-DAMO) and subsequently help to remove NO3- and decrease CH4 emission. We, therefore, investigated the effects of N input into aquifers and its effect on Fe and As mobility, by running a set of microcosm experiments using aquifer sediment from Van Phuc, Vietnam supplemented with 15NO3- and 13CH4. Additionally, we assessed the effect of N-DAMO by inoculating the sediment with two different N-DAMO enrichment cultures (N-DAMO(O) and N-DAMO(V)). We found that native microbial communities and both N-DAMO enrichments could efficiently consume nearly 5 mM NO3- in 5 days. In an uninoculated setup, NO3- was preferentially used over Fe(III) as an electron acceptor and consequently inhibited Fe(III) reduction and As mobilization. The addition of N-DAMO(O) and N-DAMO(V) enrichment cultures led to substantial Fe(III) reduction followed by the release of Fe2+ (0.190 mM and 0.350 mM, respectively) and buildup of sedimentary Fe(II) (11.20 mM and 10.91 mM, respectively) at the end of the experiment (day 64). Only in the N-DAMO(O) inoculated setup, As was mobilized (27.1 ug/L), while in the setup inoculated with N-DAMO(V) a significant amount of Mn (24.15 mg/L) was released to the water. Methane oxidation and 13CO2 formation were observed only in the inoculated setups, suggesting that the native microbial community did not have sufficient potential for N-DAMO. An increase of NH4+ implied that dissimilatory nitrate reduction to ammonium (DNRA) took place in both inoculated setups. The archaeal community in all treatments was dominated by Ca. Methanoperedens while the bacterial community consisted largely of various denitrifiers. Overall, our results suggest that input of N fertilizers to the aquifer decreases As mobility and that CH4 cannot serve as an electron donor for the native NO3- reducing community.

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“…The Ca. Methanoperedenaceae (previously named ANME-2d) are also closely related to ANME-2a/b in the phylogenetic tree, but they are able to live without partner bacteria and are largely derived from freshwater habitats 16 . The ANME-3 clade is usually dominant in methane-rich mud volcanoes 17 .…”

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confidence: 99%

The late Archaean to early Proterozoic origin and evolution of anaerobic methane‐oxidizing archaea

Wang

Xie

Hou

et al. 2022

mLife

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Impact statement Microorganisms, called anaerobic methane‐oxidizing archaea (ANME), can reduce a large amount of greenhouse gas methane and therefore have the potential to cool the Earth. We collected nearly all ANMEs genomes in public databases and performed a comprehensive comparative genomic analysis and molecular dating. Our results show that ANMEs originated in the late Archaean to early Proterozoic eon. During this period of time, our planet Earth was experiencing the Great Oxygenation Event and Huronian Glaciation, a dramatic drop in the Earth's surface temperature. This suggests that the emergence of ANMEs may contribute to the reduction of methane at that time, which is an unappreciated potential cause that led to the Huronian Glaciation.

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Lateral gene transfer drives metabolic flexibility in the anaerobic methane oxidising archaeal familyMethanoperedenaceae

Cited by 4 publications

References 110 publications

Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea

Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea

Nitrate leaching and its implication for Fe and As mobility in a Southeast Asian aquifer

The late Archaean to early Proterozoic origin and evolution of anaerobic methane‐oxidizing archaea

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