Lateral-flow immunoassays for mycotoxins and phycotoxins: a review

Anfossi, Laura; Baggiani, Claudio; Giovannoli, Cristina; D’Arco, Gilda; Giraudi, Gianfranco

doi:10.1007/s00216-012-6033-4

Cited by 178 publications

(95 citation statements)

References 67 publications

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“…Yet, this approach seems to be restricted to straightforward detection mechanisms in which the target molecule reacts in a selective manner with a component of the solution to generate a colourimetric signal. This is a limitation compared to paper-based sensors, which enable complex detection schemes such as immunoassays 22 and DNA tests. 21 In recent years nanosensors have been changing this paradigm by enabling the detection of a multitude of analytes in solution and with the naked eye (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Solution-based nanosensors for in-field detection with the naked eye

Paterson

Rica

2015

Analyst

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This version is available at https://strathprints.strath.ac.uk/52490/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the Strathprints administrator: strathprints@strath.ac.ukThe Strathprints institutional repository (https://strathprints.strath.ac.uk) is a digital archive of University of Strathclyde research outputs. It has been developed to disseminate open access research outputs, expose data about those outputs, and enable the management and persistent access to Strathclyde's intellectual output.Journal Name Nanomaterials are revolutionising analytical applications with low-cost tests that enable detecting a target molecule in a few steps and with the naked eye. With this approach, nonexperts can perform analyses on-site and without utilising electronic readers. This is advantageous in point-of-care diagnostics, in-field measurements and analyses performed in resource-constrained settings. Here we review the main strategies adopted for detecting analytes with the naked eye and at the point of need using plasmonic nanosensors, catalytic nanoparticles and fluorescent nanomaterials. Examples of the detection of ions, glucose, small molecules, peptides and proteins with the nanosensors are explained in detail. IntroductionThe design of sensors for in-field measurements has been a central issue of analytical chemistry for decades. From the diagnosis of diseases at the point of care 1 to the detection of hazardous levels of pollutants 2,3 and the identification of pathogens in food samples, 4,5 there is a growing need for obtaining accurate information about the composition of a sample rapidly and at the point of need. For many years electrochemical sensors have dominated the area of in-field sensing due to the possibility of fabricating all the elements of the sensor with well-known microfabrication techniques. 6,7 These fabrication methods generate portable, compact devices in which the transducers are directly integrated with the circuitry. 8,9 However, although microchips containing electrochemical transducers can be mass-produced, the manufacturing cost of these devices is still too high for certain applications in which the sensor cannot be reutilised or recycled. Electrical readers are also expensive, and therefore their utilisation can only be justified in routine tests, for example in diagnostic tests r...

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Section: Introductionmentioning

confidence: 99%

“…Several excellent recent reviews covering these approaches are available in the literature. 18,19,22,46 …”

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confidence: 99%

Solution-based nanosensors for in-field detection with the naked eye

Paterson

Rica

2015

Analyst

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“…Nowadays, immunochromatographic tests are successfully commercialized and sold by a number of companies, and are also described in a large number of scientific articles [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…However, the influence of these factors is described only in general terms, without demonstrating the extent to which they change the characteristics of the analysis [7]. It has been reported that a detailed analysis of individual factors, such as the composition of antibody-colloidal gold conjugates [6] and the size of nanocarriers required for immobilization of antibodies [8].…”

Section: Introductionmentioning

confidence: 99%

Degradation of Monocrotophos in Sandy Loam Soil by Aspergillus sp

Jain¹

2015

IJEE

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Analytical parameters of immunochromatographic test-systems, such as the detection limit and working range, depend on many factors. However, the process of choosing the concentration and composition of reagents applied to test strip membranes, and choosing the membranes themselves has not been systematically described for commercially produced tests or for scientific studies. Here, we present the results of a study to determine how the properties of the test system components, using mycotoxin aflatoxin B1 as an example, affect its analytical performance. A comparison of different membranes used for immunochromatography was performed as well as reagent concentrations and composition of intermolecular conjugates. Optimization of the immunochromatographic system indicated a limit of aflatoxin B1 detection equal to 0.2 ng/mL. The effectiveness of the system in testing plant extracts with minimal (twice) dilution prior to analysis was determined and the final content of methanol in the course of immunoassay was 35%.

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“…Improvements in automated image and data analysis software for novice end users are required to improve the overall rapidity of analysis. The complex toxic effects of mycotoxins pose significant health risks to humans and animals and this necessitates for effective control and surveillance procedures [5][6][7][8][9]. The initial stages in the protection against mycotoxin contamination is through the adoption of good agricultural, storage and processing practices to ensure mycotoxin levels remain negligible or as low as reasonably achievable [10].…”

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confidence: 99%

Development of a nanoarray capable of the rapid and simultaneous detection of zearalenone, T2-toxin and fumonisin

McNamee

Bravin

Rosar

et al. 2017

Talanta

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K. (2017). Development of a nanoarray capable of the rapid and simultaneous detection of zearalenone, T2-toxin and fumonisin. Talanta, 164, 368-376. DOI: 10.1016/j.talanta.2016 Published in: Talanta Document Version: Peer reviewed version Queen's University Belfast -Research Portal: Link to publication record in Queen's University Belfast Research Portal Publisher rights © 2017 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/bync-nd/4.0/ which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited. General rightsCopyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights.Take down policy The Research Portal is Queen's institutional repository that provides access to Queen's research output. Every effort has been made to ensure that content in the Research Portal does not infringe any person's rights, or applicable UK laws. If you discover content in the Research Portal that you believe breaches copyright or violates any law, please contact openaccess@qub.ac.uk. Fusarium mycotoxins such as trichothecenes, zearalenone and fumonisins occur on a worldwide basis in cereal grains, animal feeds and forages. Practical solutions for multiple mycotoxin determination in samples are required by industry and regulators for cost effective screening purposes. The feasibility of developing a novel multiplex nanoarray for the simultaneous and semi-quantitative detection of three regulated mycotoxins: zearalenone (ZEA), T2-toxin (T2) and fumonisin B1 (FUM) was examined. Additionally, the assay was also able to detect HT2 toxin and fumonisin B2 and B3 due to the cross reactivity profiles of the antibodies used. Individual mycotoxin conjugates specific to the three mycotoxins were nano-spotted onto wells of a microtitre plate. Optimisation of assay parameters and antibodies was undertaken with both individual and multiplex calibration curves generated. A competitive assay format was employed enabling a calibration curve for concentration analysis and duplicate results for up to 40 samples in 70 min for the three target mycotoxins. The characteristics and performance of the nanoarray were evaluated including sensitivity and specificity for each target. Additionally, intra and inter spotting precision, cross reactivity, matrix effects and sample analysis in maize and wheat (n=8) was performed. Sensitivity, determined as the concentration causing 50 % inhibition, was 70.1, 2.8 and 90.9 ppb in PBS, 172.4, 3.2 and 129.3 ppb in methanol, 197.4, 0.7 and 216.7 ppb in wheat and 43.6, 0.5 and 25.9 ppb in maize for ZEA, T2 and FUM respectively. Intra spotting precision was 6, 11 and 10 % for PBS and 5, 11 and 12 % for methanol for ZEA, T2 and FUM respectively. Inter spotti...

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Lateral-flow immunoassays for mycotoxins and phycotoxins: a review

Cited by 178 publications

References 67 publications

Solution-based nanosensors for in-field detection with the naked eye

Solution-based nanosensors for in-field detection with the naked eye

Degradation of Monocrotophos in Sandy Loam Soil by Aspergillus sp

Development of a nanoarray capable of the rapid and simultaneous detection of zearalenone, T2-toxin and fumonisin

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