Lateral flow immunoassay with peptide-functionalized gold nanoparticles for rapid detection of protein tyrosine phosphatase 1B

Li, Xiaotong; Zhu, Qunyan; Feng-qin, XU; Jian, Minghong; Yao, Chaoqun; Zhang, Hua

doi:10.1016/j.ab.2022.114671

Cited by 11 publications

(7 citation statements)

References 35 publications

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“…15,16 Peptides-based examples are sparsely described and generally also involve antibodies. 17,18 To our knowledge, despite their numerous advantages, only two examples of LFAs solely using peptide aptamers as recognition units have been described in the literature. 19,20…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a peptide-based lateral flow assay for the detection of the cancer biomarker Mdm2

Gosselin,

Retout,

Jabin

et al. 2024

Sens. Diagn.

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show abstract

Section: Introductionmentioning

confidence: 99%

“…17,18 To our knowledge, despite their numerous advantages, only two examples of LFAs solely using peptide aptamers as recognition units have been described in the literature. 19,20…”

Section: Introductionmentioning

confidence: 99%

Development of a peptide-based lateral flow assay for the detection of the cancer biomarker Mdm2

Gosselin,

Retout,

Jabin

et al. 2024

Sens. Diagn.

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“…Compared with most reported studies, − ,,− the proposed PEC-QCM dual-mode sensing platform exhibits lower detection limits and wider linear ranges (SI Table S1). Especially, the excellent trace assay performance (as low as 10 or 40 fM) implies that the proposed PEC-QCM dual-mode sensing platform has higher assay sensitivity than the colorimetry and fluorescence methods listed in SI Table S1, which may be attributed to the excellent properties of Fe 3 O 4 @Cu 2 O@TiO 2 nanospheres in PEC photocurrent polarity switching and QCM signal amplification processes.…”

Section: Resultsmentioning

confidence: 82%

Bifunctional Magnetic Fe₃O₄@Cu₂O@TiO₂ Nanosphere-Mediated Dual-Mode Assay of PTP1B Activity Based on Photocurrent Polarity Switching and Nanozyme-Engineered Biocatalytic Precipitation Strategies

Zhang

Yang

et al. 2022

Anal. Chem.

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Dysregulation of protein phosphatases is associated with the progression of various human diseases and cancers. Herein, a photoelectrochemical (PEC)-quartz crystal microbalance (QCM) dual-mode sensing platform was developed for protein tyrosine phosphatase 1B (PTP1B) activity assay based on bifunctional magnetic Fe 3 O 4 @Cu 2 O@TiO 2 nanosphere-mediated PEC photocurrent polarity switching and QCM signal amplification strategies. The PTP1B-specific phosphopeptide (P-peptide) with a cysteine end was designed and immobilized onto the QCM Au chip via the Au−S bond. Subsequently, the Fe 3 O 4 @Cu 2 O@TiO 2 nanosphere was connected to the P-peptide via the specific interaction between the phosphate group on the P-peptide and TiO 2 . After incubation with PTP1B, the dephosphorylation of the Ppeptide occurred, causing some Fe 3 O 4 @Cu 2 O@TiO 2 nanospheres to be released from the chip surface. The released magnetic Fe 3 O 4 @Cu 2 O@TiO 2 nanospheres (labeled as R-Fe 3 O 4 @Cu 2 O@TiO 2 ) were quickly separated via magnetic separation technology and attached to the Bi 2 S 3 -decorated magnetic indium−tin oxide (Bi 2 S 3 /MITO) electrode by magnetic force, inducing the switch of the photocurrent polarity of the electrode from anodic current (the Bi 2 S 3 /MITO electrode) to cathodic current (the R-Fe 3 O 4 @ Cu 2 O@TiO 2 /Bi 2 S 3 /MITO electrode). Also, the nondephosphorylated P-peptide linked Fe 3 O 4 @Cu 2 O@TiO 2 nanospheres as nanozymes with horseradish peroxidase activity to catalyze the formation of precipitation on the surface of the Au chip, leading to a frequency change of the QCM. Thus, the proposed PEC-QCM dual-mode sensing platform achieved accurate and reliable assay of PTP1B activity because of the different mechanisms and independent signal transductions. In addition, this dual-mode sensing platform can be easily extended for other protein phosphatase activity analysis and shows great potential in the early diagnosis of the protein phosphatase-related diseases and the protein phosphatase-targeted drug discovery.

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“…Usually, preparations of GNPs with a diameter of about 30–40 nm are considered as preferable for LFIA [ 40 ]. However, the advantages of smaller GNPs for competitive schemes were sometimes noted–13 nm [ 41 , 42 ], 15 nm [ 43 ], and 20 nm [ 44 ]. The use of larger GNPs in LFIA could be associated with unproductive competition, as described in [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

et al. 2022

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The use of a large amount of toxic synthetic materials leads to an increase in the pollution of environmental objects. Phthalates are compounds structurally related to esters of phthalic acid that are widely used in the manufacturing of synthetic packaging materials as plasticizers. Their danger is conditioned by leaching into the environment and penetrating into living organisms with negative consequences and effects on various organs and tissues. This work presents the first development of lateral flow immunoassay to detect dibutyl phthalate, one of the most common representatives of the phthalates group. To form a test zone, a hapten–protein conjugate was synthesized, and gold nanoparticles conjugated with antibodies to dibutyl phthalate were used as a detecting conjugate. The work includes the preparation of immunoreagents, selectivity investigation, and the study of the characteristics of the medium providing a reliable optical signal. Under the selected conditions for the analysis, the detection limit was 33.4 ng/mL, and the working range of the determined concentrations was from 42.4 to 1500 ng/mL. Time of the assay–15 min. The developed technique was successfully applied to detect dibutyl phthalate in natural waters with recovery rates from 75 to 115%.

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Lateral flow immunoassay with peptide-functionalized gold nanoparticles for rapid detection of protein tyrosine phosphatase 1B

Cited by 11 publications

References 35 publications

Development of a peptide-based lateral flow assay for the detection of the cancer biomarker Mdm2

Development of a peptide-based lateral flow assay for the detection of the cancer biomarker Mdm2

Bifunctional Magnetic Fe₃O₄@Cu₂O@TiO₂ Nanosphere-Mediated Dual-Mode Assay of PTP1B Activity Based on Photocurrent Polarity Switching and Nanozyme-Engineered Biocatalytic Precipitation Strategies

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

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Lateral flow immunoassay with peptide-functionalized gold nanoparticles for rapid detection of protein tyrosine phosphatase 1B

Cited by 11 publications

References 35 publications

Development of a peptide-based lateral flow assay for the detection of the cancer biomarker Mdm2

Development of a peptide-based lateral flow assay for the detection of the cancer biomarker Mdm2

Bifunctional Magnetic Fe3O4@Cu2O@TiO2 Nanosphere-Mediated Dual-Mode Assay of PTP1B Activity Based on Photocurrent Polarity Switching and Nanozyme-Engineered Biocatalytic Precipitation Strategies

Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

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Bifunctional Magnetic Fe₃O₄@Cu₂O@TiO₂ Nanosphere-Mediated Dual-Mode Assay of PTP1B Activity Based on Photocurrent Polarity Switching and Nanozyme-Engineered Biocatalytic Precipitation Strategies