Lateral flow immunoassay based on dual spectral-overlapped fluorescence quenching of polydopamine nanospheres for sensitive detection of sulfamethazine

The purpose of this research was to study the biological activity of probiotic against MDR bacteria that isolated from diabetic foot infections. During the months of October 2020 to March 2021, a total of 65 specimens and investigations were collected. Specimens for diabetic foot infection were gathered from two hospitals in the province of AL-Najaf (AL-Sadder Medical City and AL-Hakeem Hospital). Swabs from diabetic foot infection patients were collected and sent to the lab, where they were streaked on MacConkey agar, a blood agar., around 40 (61.5%) exhibit positive bacterial growth culture, whereas 25 (38.5%) show negative findings for culturing. Negative growth might be due to other infection-causing agents such as anaerobic bacteria, fungi, and viruses [1]. Gram-negative bacteria had a high rate of 42 (64.4%) based on morphological and biochemical tests, with B. cepacia having a high percentage of 14 (33.3%) and A. baumanni having a high percentage of 12 (28.7%). K. pneumonia has 7 (16.6%), E. cloacae has 6 (14.4%), and no growth in 3 (7%) as shown in figure (4-2).

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“…The elution buffer will be consumed by the matrix for 2 minutes. The pure DNA eluted by centrifugation at 16000 rpm for 2 min [5].…”

Section: Extraction Of Dna For Sequencingmentioning

confidence: 99%

“…For producing various types of nanoparticles, a variety of physical, chemical, and biological processes are available, some of which are more costly, energyintensive, and possibly harmful to the environment [5]. The synthesis of green nanoparticles is an interesting topic of nanoscience and nanotechnology.…”

Section: Introductionmentioning

confidence: 99%

Selenium Nano-particles bio-synthesized by pathogenic bacteria having antibiofilm activity analysed by fluorescent microscopy and Molecular detection of antibiotic resistance genes

Hadi

Aldujaili

2022

ijhs

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“…At present, the main methods used for the analysis of SAs in animal-derived foods are high-performance liquid chromatography–mass spectrometry (HPLC–MS), ,− immunoassay including enzyme linked immunosorbent assay (ELISA), ,− and DNA aptamer-based methods including colorimetric, electrochemical, fluorescent, and chemiluminescent methods. − Among the above methods, HPLC–MS has capability of simultaneously determining multiple SAs with high sensitivity, reliability and better reproducibility. ,− However, HPLC–MS methods require costly apparatus and a longer analysis time, thus making the on-site screening of SAs residues in animal-derived foods impossible. Immunoassays especially ELISA methods do not required apparatus and complicated pretreatment and have better specificity and a short detection time.…”

Section: Introductionmentioning

confidence: 99%

“…Immunoassays especially ELISA methods do not required apparatus and complicated pretreatment and have better specificity and a short detection time. Especially, some ELISA methods previously reported are capable of simultaneously detecting multiple SAs by employing broad-specificity antibody as receptor, ,− which meets the requirement of on-site screening of SAs residues in animal-derived foods. However, the employment of an antibody makes immunoassays have obvious weaknesses such as the dependence on the surrounding environment, higher cost, poorer stability, and risk of a false positive result.…”

Section: Introductionmentioning

confidence: 99%

Broad-Specificity Aptamer of Sulfonamides: Isolation and Its Application in Simultaneous Detection of Multiple Sulfonamides in Fish Sample

Chen

Huang

et al. 2022

J. Agric. Food Chem.

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Sulfonamide antibiotics (SAs) are widely used in animal husbandry and aquaculture, and the excess residues of SAs in animal-derived foods will harm the health of consumers. In reality, various SAs were alternately used in animal husbandry and aquaculture, and thus, it is urgent need to develop simple and high-throughput methods for simultaneously detecting multiple SAs or groups of SAs in order to realize rapid screening of total SAs residues in animal-derived foods. We herein isolated a broad-specificity aptamer for SAs by using a multi-SAs systematic evolution of ligands by exponential enrichment (SELEX) strategy. The isolated broad-specificity aptamer has a higher binding affinity to five different SAs including sulfaquinoxaline (SQ), sulfamethoxypyridazine (SMPZ), sulfametoxydiazine (SMD), sulfachloropyridazine (SCP), and sulfapyridine (SPD) and, thus, can be used as a bioreceptor for developing various high-throughput methods for the simultaneous detection or rapid screening of above five SAs. Based on the isolated broad-specificity aptamer and Cy7 (diethylthiatricarbocyanine) displacement strategy, a colorimetric aptasensor was developed for the simultaneous detection of SQ, SMPZ, SMD, SCP, and SPD with a visual detection limit of 2.0–5.0 μM and a spectrometry detection limit of 0.2–0.5 μM. The colorimetric aptasensor was successfully used to detect SQ, SMPZ, SMD, SCP, and SPD in fish muscle with a recovery of 82%–92% and a RSD (n = 5) < 7%. The success of this study provided a promising bioreceptor for developing various high-throughput methods for on-site rapid screening of multiple SAs residues, as well as a simple method for the rapid and cost-effective screening of total SQ, SMPZ, SMD, SCP, and SPD in seafood.

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“…Immunochromatographic assays (ICAs) have unique advantages, such as simplicity, rapidness, low cost, and ease of use. They have been widely employed for on-site detection Wang et al, 2022). In an ICA, label signal reporters are important because of detection sensitivity and selectivity .…”

Section: Introductionmentioning

confidence: 99%

Quantum dot nanobeads as multicolor labels for simultaneous multiplex immunochromatography detection of four nitrofuran metabolites in aquatic products

Liu

Cheng

Xia

et al. 2022

Preprint

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A multicolor immunochromatography assay platform based on quantum dot nanobeads (QBs) for the rapid and simultaneous detection of nitrofuran metabolites in different aquatic products is documented. These metabolites include 3-amino-2-oxazolidinone (AOZ), 1-aminohydantoin (AHD), semicarbazide (SEM), and 3-amino-5-morpholino-methyl-1,3-oxazolidinone (AMOZ). QBs with emission colors of red, yellow, green, and orange were employed and functionalized with the corresponding antibodies to each analyte to develop a multicolor channel. The visual detection limits (cut-off values) of our method for AOZ, AHD, SEM, and AMOZ reached up to 50 ng/mL, which were 2, 20, 20, and 20 times lower than those of traditional colloidal gold test strips, respectively. The test strip is capable of detection within 10 min in real samples while still achieving good stability and specificity. These results demonstrate that the developed multicolor immunochromatography assay platform is a promising technique for multiplex, highly sensitive, and on-site detection of nitrofuran metabolites in aquatic products.

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Lateral flow immunoassay based on dual spectral-overlapped fluorescence quenching of polydopamine nanospheres for sensitive detection of sulfamethazine

Cited by 59 publications

References 56 publications

Selenium Nano-particles bio-synthesized by pathogenic bacteria having antibiofilm activity analysed by fluorescent microscopy and Molecular detection of antibiotic resistance genes

Selenium Nano-particles bio-synthesized by pathogenic bacteria having antibiofilm activity analysed by fluorescent microscopy and Molecular detection of antibiotic resistance genes

Broad-Specificity Aptamer of Sulfonamides: Isolation and Its Application in Simultaneous Detection of Multiple Sulfonamides in Fish Sample

Quantum dot nanobeads as multicolor labels for simultaneous multiplex immunochromatography detection of four nitrofuran metabolites in aquatic products

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