Lateral Flow Devices

Bandla, Murali; Thompson, Rick; Shan, Guomin

doi:10.1002/9780470909935.ch5

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…A single LFS can accommodate more than one antibody, and LFS exist that can detect up to four proteins simultaneously. Individual LFS can also be assembled into multi-analyte combs according to diagnostic needs . Disadvantages of LFS in comparison to ELISA include lower sensitivity and higher relative cost in some cases.…”

Section: Assessment Of Dna- and Protein-based Detection Methodsmentioning

confidence: 99%

Application of DNA- and Protein-Based Detection Methods in Agricultural Biotechnology

Alarcon

Shan

Layton³

et al. 2018

J. Agric. Food Chem.

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DNA-and protein-based detection methods are widely used tools for monitoring biotechnology-derived crops and their products globally. Agricultural biotechnology companies, food/feed suppliers and supply chains, diagnostic testing companies, and regulatory authorities heavily rely on these two technologies for product development, seed production, compliance, and contractual needs. The primary use of DNA-and protein-based detection methods is either to verify the presence or absence of genetically engineered (GE) materials or to quantify the amount of GE material present in a product. This review describes key parameters of DNA-and protein-based detection methods, and thorough assessment of their applications and their advantage and limitations in agricultural biotechnology are discussed in detail. The review highlights the principle and considerations of detection method selection, which will equip users to choose suitable technology and obtain reliable test results. The review also compares the compatibility of the two technologies in GE product testing using a case study. KEYWORDS: genetically modified (GM), genetically modified organism (GMO), genetically engineered (GE), GM food testing, food analysis, DNA detection methods, protein detection methods, Bioengineered (BE)

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Section: Assessment Of Dna- and Protein-based Detection Methodsmentioning

confidence: 99%

Application of DNA- and Protein-Based Detection Methods in Agricultural Biotechnology

Alarcon

Shan

Layton³

et al. 2018

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

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Proteomic Selection of Immunodiagnostic Antigens for Human African Trypanosomiasis and Generation of a Prototype Lateral Flow Immunodiagnostic Device

Sullivan

Wall²,

Carrington

et al. 2013

PLoS Negl Trop Dis

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BackgroundThe diagnosis of Human African Trypanosomiasis relies mainly on the Card Agglutination Test for Trypanosomiasis (CATT). While this test is successful, it is acknowledged that there may be room for improvement. Our aim was to develop a prototype lateral flow test based on the detection of antibodies to trypanosome antigens.Methodology/Principal FindingsWe took a non-biased approach to identify potential immunodiagnostic parasite protein antigens. The IgG fractions from the sera from Trypanosoma brucei gambiense infected and control patients were isolated using protein-G affinity chromatography and then immobilized on Sepharose beads. The IgG-beads were incubated with detergent lysates of trypanosomes and those proteins that bound were identified by mass spectrometry-based proteomic methods. This approach provided a list of twenty-four trypanosome proteins that selectively bound to the infection IgG fraction and that might, therefore, be considered as immunodiagnostic antigens. We selected four antigens from this list (ISG64, ISG65, ISG75 and GRESAG4) and performed protein expression trials in E. coli with twelve constructs. Seven soluble recombinant protein products (three for ISG64, two for ISG65 and one each for ISG75 and GRESAG4) were obtained and assessed for their immunodiagnostic potential by ELISA using individual and/or pooled patient sera. The ISG65 and ISG64 construct ELISAs performed well with respect to detecting T. b. gambiense infections, though less well for detecting T. b. rhodesiense infections, and the best performing ISG65 construct was used to develop a prototype lateral flow diagnostic device.Conclusions/SignificanceUsing a panel of eighty randomized T. b. gambiense infection and control sera, the prototype showed reasonable sensitivity (88%) and specificity (93%) using visual readout in detecting T. b. gambiense infections. These results provide encouragement to further develop and optimize the lateral flow device for clinical use.

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Lateral Flow Devices

Cited by 2 publications

References 37 publications

Application of DNA- and Protein-Based Detection Methods in Agricultural Biotechnology

Application of DNA- and Protein-Based Detection Methods in Agricultural Biotechnology

Proteomic Selection of Immunodiagnostic Antigens for Human African Trypanosomiasis and Generation of a Prototype Lateral Flow Immunodiagnostic Device

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