Lateral flow assay of pathogenic viruses and bacteria in healthcare

Nan, Xuanxu; Yao, Xianhua; Li, Yang; Cui, Yue

doi:10.1039/d3an00719g

Cited by 3 publications

(1 citation statement)

References 214 publications

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“…LFAs, particularly when deployed in field settings, prove to be highly advantageous as screening tools, rapidly identifying samples warranting further testing within 20 mins (Nan et al 2023). With their quick turnaround time, LFAs enable high throughput testing without prolonged incubation periods or waiting times, offering an attractive alternative to conventional screening methods.…”

Section: Discussionmentioning

confidence: 99%

Application of chimeric antigens to paper-based diagnostics for detection of West Nile virus infections ofCrocodylus porosus –a novel animal test case

Johnston,

Habarugira,

Harrison

et al. 2024

Preprint

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Laboratory-based diagnostics like plaque reduction neutralization tests (PRNT) and ELISA are commonly used to detect seroconversion to flavivirus infections. However, faster, qualitative screening methods are needed for quicker diagnosis and better patient outcomes. Lateral flow assays (LFAs) can provide rapid results (5-15 mins) at the point-of-care, yet few commercial flavivirus antibody detection LFAs are available. We developed an LFA using novel chimeric viral antigens produced by genetically modifying the mosquito restricted Binjari virus (BinJV) to display the outer virion proteins of pathogenic viruses such as West Nile virus (WNV). The BinJV chimeric platform offers various advantages for diagnostic assay development, including rapid construction of new chimeras in response to emerging viral variants, safe, scalable antigen manufacturing, and structural indistinguishability to the wild-type pathogenic virion. As a demonstration of feasibility, we applied chimeric WNV (BinJV/WNV) antigen to LFA as the capture/test line reagent for detection of seroconversion of crocodilians to WNV – a virus affecting crocodilians on multiple continents. We verified the antigenic conservation of the chimera when applied to the LFA detection surface using monoclonal antibodies. Using well-characterised sera (n=60) from WNV seropositive or flavivirus naive Australian saltwater crocodiles (Crocodylus porosus), we illustrated 100% sensitivity and specificity, with results achieved in less than 15 minutes. The LFA further accurately detected seroconversion in animals experimentally infected with WNV. This qualitative screening method can be performed both inside and outside of a laboratory, and the assay design will guide the optimization of similar tests for vector borne virus infection detection in both humans and other animals.

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Section: Discussionmentioning

confidence: 99%