Lateral flow assay modified with time-delay wax barriers as a sensitivity and signal enhancement strategy

Sena‐Torralba, Amadeo; Ngo, Duy Ba; Parolo, Claudio; Hu, Liming; Álvarez-Diduk, Ruslán; Bergua, José Francisco; Rosati, Giulio; Surareungchai, Werasak; Merkoçi, Arben

doi:10.1016/j.bios.2020.112559

Cited by 53 publications

(54 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concept of using PVA dam to enhance the sensitivity of cellulose fiber-based LFA The sample flow rate has a strong effect on the sensitivity (Sena-Torralba et al 2020). Effective strategies to slow down the flow rate can enhance the sensitivity of LFA devices (Rivas et al 2014;Sena-Torralba et al 2020).…”

Section: Resultsmentioning

confidence: 99%

Improving the sensitivity of cellulose fiber-based lateral flow assay by incorporating a water-dissolvable polyvinyl alcohol dam

Alam

et al. 2021

Cellulose

View full text Add to dashboard Cite

Lateral flow assay (LFA) is an important point-of-care (POC) test platform due to the associated portability, on-site testing, and low cost for diagnosis of pathogen infections and disease biomarkers. However, compared to high-end analyzers in hospitals, LFA devices, in particular, paper-based LFA tests, fall short in accuracy. This study focuses on two ways to improve LFAs: (1) using cellulose fibers, rather than glass fibers for a sample pad, and (2) incorporating a one-step simple, facile, and low cost PVA dam into the LFA. Both strategies (cellulose fiber as a sample pad and water dissolvable PVA dam) contributed to delaying the controlled biomolecule's flow through the nitrocellulose membrane's capillary channels resulting in increased bio-recognition time, thus contributing to the enhancement of LFA sensitivity. PVA modified cellulose fiber-based LFA demonstrated 10 times higher sensitivity than the cellulose fiber-based unmodified LFA, whereas 2 times enhancement was obtained in the cellulose fiber-based sample pad LFA compared to the glass fiber-based sample pad LFA. Ultimately, 20 times increase in sensitivity was achieved in the modified LFA device. This study shows that PVA and eco-friendly cellulose fibers could be incorporated into other paper based POC testing devices for future development.

show abstract

Section: Resultsmentioning

confidence: 99%

Improving the sensitivity of cellulose fiber-based lateral flow assay by incorporating a water-dissolvable polyvinyl alcohol dam

Alam

et al. 2021

Cellulose

View full text Add to dashboard Cite

show abstract

“…Soluble wax barriers were printed on the NC surface, then melted to penetrate NC pores to create hydrophobic barriers at 1 mm after the test line, temporarily accumulating the target and label nanoparticles on top of the test line ( Figure 9 D). The wax barriers modulated the internal incubation step of the LFAs to 12 min, promoting immune-complex formation that achieved LOD of 41.19 ng/mL, which was a 51.7-fold sensitivity enhancement, and up to a 96% signal enhancement compared to the unmodified LFA for Human IgG (H-IgG) detection [ 192 ]. No full-wax barriers were printed onto the NC membrane, but several wax pillar patterns were printed onto the nitrocellulose membrane in order to produce delays as well as pseudo-turbulence in the microcapillary flow—a sensitivity improvement of almost threefold compared to the sensitivity of a conventional free-barrier LFA [ 199 ].…”

Section: Pads Engineering-based Signal Amplificationmentioning

confidence: 99%

“…Adapted with permission from Ref. [ 192 ]. ( E ) Laser-based direct-write procedure for localized deposition of a photopolymer to create polymerized hydrophobic structures in the porous substrate.…”

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

Advanced Signal-Amplification Strategies for Paper-Based Analytical Devices: A Comprehensive Review

Hoang

Phan

et al. 2021

Biomedicines

View full text Add to dashboard Cite

Paper-based analytical devices (PADs) have emerged as a promising approach to point-of-care (POC) detection applications in biomedical and clinical diagnosis owing to their advantages, including cost-effectiveness, ease of use, and rapid responses as well as for being equipment-free, disposable, and user-friendly. However, the overall sensitivity of PADs still remains weak, posing a challenge for biosensing scientists exploiting them in clinical applications. This review comprehensively summarizes the current applicable potential of PADs, focusing on total signal-amplification strategies that have been applied widely in PADs involving colorimetry, luminescence, surface-enhanced Raman scattering, photoacoustic, photothermal, and photoelectrochemical methods as well as nucleic acid-mediated PAD modifications. The advances in signal-amplification strategies in terms of signal-enhancing principles, sensitivity, and time reactions are discussed in detail to provide an overview of these approaches to using PADs in biosensing applications. Furthermore, a comparison of these methods summarizes the potential for scientists to develop superior PADs. This review serves as a useful inside look at the current progress and prospective directions in using PADs for clinical diagnostics and provides a better source of reference for further investigations, as well as innovations, in the POC diagnostics field.

show abstract

“…The biosensor sensitivity is of great importance to detect pathogens in environmental, agrifood, or biological samples because pathogens are typically present in very low concentrations. Therefore, various diagnostic assays using functionalized nanoparticles to amplify the signal and boost the sensitivity have been reported in recent years [25][26][27][28]. Herein, we propose to evaluate the sensitivity increase of MELISA for Influenza A detection via the nucleoprotein A, using immunomagnetic beads for the capture step and biotin-coated nanoparticles (biotin-NP) to enhance the signal at the detection step.…”

Section: Introductionmentioning

confidence: 99%

Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay

et al. 2020

View full text Add to dashboard Cite

This study reports the development of a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the panreactive detection of the Influenza A virus. The assay combines immunomagnetic beads and biotin-nanoparticle-based detection to quantify a highly conserved viral nucleoprotein in virus lysates. At the capture step, monoclonal antibody-coated magnetic microbeads were used to bind and concentrate the nucleoprotein in samples. The colorimetric detection signal was amplified using biotinylated silica nanoparticles (NP). These nanoparticles were functionalized on the surface with short DNA spacers bearing biotin groups by an automated supported synthesis method performed on nano-on-micro assemblies with a DNA/RNA synthesizer. A biotin-nanoparticle and immunomagnetic bead-based assay was developed. We succeeded in detecting Influenza A viruses directly in the lysis buffer supplemented with 10% saliva to simulate the clinical context. The biotin-nanoparticle amplification step enabled detection limits as low as 3 × 10 3 PFU mL −1 and 4 × 10 4 PFU mL −1 to be achieved for the H1N1 and H3N2 strains respectively. In contrast, a classical ELISA test based on the same antibody sandwich showed detection limit of 1.2 × 10 7 PFU mL −1 for H1N1. The new enhanced MELISA proved to be specific, as no cross-reactivity was found with a porcine respiratory virus (PRRSV).

show abstract

Lateral flow assay modified with time-delay wax barriers as a sensitivity and signal enhancement strategy

Cited by 53 publications

References 81 publications

Improving the sensitivity of cellulose fiber-based lateral flow assay by incorporating a water-dissolvable polyvinyl alcohol dam

Improving the sensitivity of cellulose fiber-based lateral flow assay by incorporating a water-dissolvable polyvinyl alcohol dam

Advanced Signal-Amplification Strategies for Paper-Based Analytical Devices: A Comprehensive Review

Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay

Contact Info

Product

Resources

About