Lateral flow aptamer assay integrated smartphone-based portable device for simultaneous detection of multiple targets using upconversion nanoparticles

Jin, Birui; Yang, Yexin; He, Rongyan; Park, Yong Il; Lee, Ruda; Bai, Dan; Li, Fēi; Lu, Tian Jian; Xu, Feng; Lin, Min

doi:10.1016/j.snb.2018.08.074

Cited by 122 publications

(48 citation statements)

References 36 publications

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“…The sensor strip has a sensing area and a reference area ( Figure 1 , bottom part ). The sensing pad is made of a dry, porous, and absorbent nitrocellulose blotting filter paper impregnated with all reagents, which resulted in a built-in capacity to drive a \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$30~\mu \text{L}$ \end{document} sample by capillary forces without spilling, and rapidly separating large components such as proteins from soluble ions [22] . Reagent A strips Fe(III) from transferrin, reduces Fe(III) to Fe(II), and chelates potential interferents such as Cu(II), whereas reagent B (ferene) chelates Fe(II) to form the colored complex.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…Due to the above-mentioned limitations, we present here a low-cost novel assay for detection of iron based on storable, dry, and disposable sensor strips and a smartphone mount and application to reduce the need of laboratory space and special instrumentation and to conduct all analyses at room temperature at a low manufacturing and end-user cost. We choose the smartphone as a detection platform since the ever-increasing number of smartphone owners (3 billion+ as of 2020) opens the possibility to deliver low-cost detectors everywhere with proven capabilities of complex imaging algorithms for clinical applications [16] – [23] .…”

Section: Introductionmentioning

confidence: 99%

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Total Iron Measurement in Human Serum With a Novel Smartphone-Based Assay

Serhan

Jackemeyer

Long

et al. 2020

IEEE J. Transl. Eng. Health Med.

130

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Background: Abnormally low or high blood iron levels are common health conditions worldwide and can seriously affect an individual's overall well-being. A low-cost point-of-care technology that measures blood iron markers with a goal of both preventing and treating iron-related disorders represents a significant advancement in medical care delivery systems. Methods A novel assay equipped with an accurate, storable, and robust dry sensor strip, as well as a smartphone mount and (iPhone) app is used to measure total iron in human serum. The sensor strip has a vertical flow design and is based on an optimized chemical reaction. The reaction strips iron ions from blood-transport proteins, reduces Fe(III) to Fe(II), and chelates Fe(II) with ferene, with the change indicated by a blue color on the strip. The smartphone mount is robust and controls the light source of the color reading App, which is calibrated to obtain output iron concentration results. The real serum samples are then used to assess iron concentrations from the new assay, and validated through intra-laboratory and inter-laboratory experiments. The intra-laboratory validation uses an optimized iron detection assay with multi-well plate spectrophotometry. The inter-laboratory validation method is performed in a commercial testing facility (LabCorp). Results: The novel assay with the dry sensor strip and smartphone mount, and App is seen to be sensitive to iron detection with a dynamic range of 50-300 μg/dL, sensitivity of 0.00049 a.u/μg/dL, coefficient of variation (CV) of 10.5%, and an estimated detection limit of ~15 μg/dL These analytical specifications are useful for predicting iron deficiency and overloads. The optimized reference method has a sensitivity of 0.00093 a.u/μg/dL and CV of 2.2%. The correlation of serum iron concentrations (N=20) between the optimized reference method and the novel assay renders a slope of 0.95, and a regression coefficient of 0.98, suggesting that the new assay is accurate. Last, a spectrophotometric study of the iron detection reaction kinetics is seen to reveal the reaction order for iron and chelating agent. Conclusion: The new assay is able to provide accurate results in intra-and inter-laboraty validations, and has promising features of both mobility and low-cost manufacturing suitable for global healthcare settings.

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Section: Experimental Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Total Iron Measurement in Human Serum With a Novel Smartphone-Based Assay

Serhan

Jackemeyer

Long

et al. 2020

IEEE J. Transl. Eng. Health Med.

130

View full text Add to dashboard Cite

show abstract

“…Recently, UCNPs have gained attention as signal reporters due to low background signals, unique luminescent properties, low signal-to-noise ratios, high resistance to photobleaching, and high sensitivity in both in vivo and in vitro applications. Using this principle, Jin et al developed a lateral flow aptamer assay (LFAA: shown in Figure 7) for the simultaneous detection of multiple targets of interest using a smartphone-based portable device (Jin et al, 2018). In their design, three different aminomodified aptamer sequences exhibiting high specificity for mercury, ochratoxin A, and Salmonella were covalently bound to red, green, and blue UCNPs, respectively, to form aptamermulticolored UCNP probes.…”

Section: Multiplex Detection Of Environmental Contaminants In Watermentioning

confidence: 99%

“…FIGURE 7 | Schematic representation of the lateral-flow aptamer assay developed for the detection of multiple analytes in environmental water samples (Jin et al, 2018).…”

Section: Monitoring Lead In Soil Using Aptamer-based Biosensorsmentioning

confidence: 99%

Aptamer-Based Biosensors for Environmental Monitoring

2020

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Due to their relative synthetic and chemical simplicity compared to antibodies, aptamers afford enhanced stability and functionality for the detection of environmental contaminants and for use in environmental monitoring. Furthermore, nucleic acid aptamers can be selected for toxic targets which may prove difficult for antibody development. Of particular relevance, aptamers have been selected and used to develop biosensors for environmental contaminants such as heavy metals, small-molecule agricultural toxins, and water-borne bacterial pathogens. This review will focus on recent aptamer-based developments for the detection of diverse environmental contaminants. Within this domain, aptamers have been combined with other technologies to develop biosensors with various signal outputs. The goal of much of this work is to develop cost-effective, user-friendly detection methods that can complement or replace traditional environmental monitoring strategies. This review will highlight recent examples in this area. Additionally, with innovative developments such as wearable devices, sentinel materials, and lab-on-a-chip designs, there exists significant potential for the development of multifunctional aptamer-based biosensors for environmental monitoring. Examples of these technologies will also be highlighted. Finally, a critical perspective on the field, and thoughts on future research directions will be offered.

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“…Recognition aptamers present on GNPs and capture aptamers immobilized on the capture lines should have different binding sites to increase the sensitivity. Some LFAs for Salmonella detection based on aptamers are recorded in the literature with various reagents and techniques [35,36]. While some aptamers show lower LOD such as 10 1 colony forming unit (cfu) of S. enteritidis [37], some of them show higher.…”

Section: Antibodies For Lfasmentioning

confidence: 99%

Lateral Flow Assay for Salmonella Detection and Potential Reagents

Çam¹

2020

New Insight Into Brucella Infection and Foodborne Diseases

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Salmonella is among the very important pathogens threating human and animal health. It is a common food pathogen transmitted from animals to humans via contaminated food, drinking water, and air. It invades the intestinal tract of hosts and causes salmonellosis leading to death. S. enteritidis was the most common species accounted for all salmonellosis cases. S. typhimurium is also another significant species causing the serious cases worldwide. To ensure public health, early detection of pathogens is crucial. Lateral flow assay (LFA), immunochromatographic assay, is a simple and rapid diagnostic test kits used in various fields and can be developed by, aptamers, antibodies (Abs), and nucleic acids. They are also being continued to develop different capture reagents coming from the recombinant technology. It has many advantages such as having mature technology, market presence, low cost, easy to use for end users without education, and stable shelf life. Gold nanoparticles (GNPs) are the most commonly used labels in the LFAs for the naked-eye analysis. Therefore, Salmonella detection by LFA based on GNPs in a rapid and simple way is always open to be developed by new reagents and methods.

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Lateral flow aptamer assay integrated smartphone-based portable device for simultaneous detection of multiple targets using upconversion nanoparticles

Cited by 122 publications

References 36 publications

Total Iron Measurement in Human Serum With a Novel Smartphone-Based Assay

Total Iron Measurement in Human Serum With a Novel Smartphone-Based Assay

Aptamer-Based Biosensors for Environmental Monitoring

Lateral Flow Assay for Salmonella Detection and Potential Reagents

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