Latent Syphilis: Immunoglobulins Reactive in Immunofluorescence and Other Serological Tests

Julian, Alfredo J.; Logan, Leslie C.; Norins, Leslie C.; Scotti, Angelo

doi:10.1128/iai.3.4.559-561.1971

Cited by 14 publications

(10 citation statements)

References 5 publications

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“…In the light of Hederstedt's work, our previous immunofluorescence studies of antibodies in early syphilis (9), and the current study, we can assume a uniform concept of action wherein the FTA-ABS and TPI tests gain their apparent specificity. Both assays incorporate factors which remove, suppress, or inactivate natural anti-T. pallidum reactivity in the sera of normal people, while allowing the recognition of immune antibodies in infected individuals.…”

Section: Downloaded Frommentioning

confidence: 62%

“…Gel filtration. Sera or Cohn fractions were fractionated by gel filtration through a bed of Sephadex G-200, 90 X 2.5 cm, as previously described (9).…”

Section: Materlkls and Methodsmentioning

confidence: 99%

“…After incubation and rinsing, attachment of immunoglobulins to the organism was revealed by "staining" with fluoresceinlabeled goat antisera (Hyland) specific for human IgG, IgM, or IgA, and the slide was examined with a fluorescence microscope. The detailed evidence for the monospecificity of each reagent, used at its working dilution, has been presented elsewhere (9).…”

Section: Materlkls and Methodsmentioning

confidence: 99%

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Immunoglobulin Class of Natural Human Antibodies Reactive with Treponema pallidum

Julian¹,

Logan²,

Norins³

et al. 1970

Infect Immun

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To aid the development of serological tests for incubating syphilis, a characterization was made of the background of natural anti-Treponema pallidum antibodies against which the initial immune response to syphilis takes place. Sera from presumed normal persons were studied with monospecific antisera in an indirect fluorescent-antibody procedure. Of 36 sera tested at a 1:5 dilution, all showed IgG reactivity with T. pallidum (Nichols strain), 58% showed IgM reactivity, and 20% showed IgA reactivity. The titer of IgG reactivity was considerably higher than that of the other two immunoglobulins. Heating the sera for 1 hr at 65 C abolished IgA and IgM anti-T. pallidum reactivity, but one-third of the sera retained IgG reactivity. Human Cohn fractions II and III, from three commercial sources contained mostly IgG antibodies reactive with T. pallidum, but IgM and IgA antibodies were also present. IgG reactivity was found in a pool of presumably normal maternal sera from 20 mothers and in a corresponding pool of umbilical cord serum from their infants. IgM and IgA reactivities were present only in the maternal serum pool.

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confidence: 62%

“…Gel filtration. Sera or Cohn fractions were fractionated by gel filtration through a bed of Sephadex G-200, 90 X 2.5 cm, as previously described (9).…”

Section: Materlkls and Methodsmentioning

confidence: 99%

Section: Materlkls and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Immunoglobulin Class of Natural Human Antibodies Reactive with Treponema pallidum

Julian¹,

Logan²,

Norins³

et al. 1970

Infect Immun

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“…The conjugate was absorbed with Treponema phagedenis biotype Reiter with 1 part packed cells to 5 parts conjugate. The conjugate and cells were incubated at 37°C for 2 h, followed by holding at 2 to 8°C overnight (3). The bacterial cells were removed by centrifugation at 17,300 x g for 30 min, and the supernatant was filtered with a 0.45-ltm Millipore membrane filter.…”

Section: Methodsmentioning

confidence: 99%

Fluorescent treponemal antibody-absorption double-staining procedure

Hunter¹,

Pender²,

Kennedy³

et al. 1981

J Clin Microbiol

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The fluorescent treponemal antibody-absorption double-staining step-by-step procedure and proposed reference reagents for the test are described. The test and reagents were evaluated in two separate laboratories on 265 fresh sera, and test results were compared with the reference fluorescent treponemal antibody-absorption test results performed in a third laboratory. The data indicate that the tests are comparable in the areas where the test is recommended for use. Problems with inadequate light filtration occurred, but these could be resolved. This test is recommended for use with microscopes equipped with incident illumination.

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“…In syphilis, as in many other infectious diseases, the early antibody response to infection is the production of IgM, rapidly followed by the appearance of IgG, which soon becomes predominant (18). In current studies, several workers have shown that the measurement of specific IgM antibodies to T.p.…”

Section: Discussionmentioning

confidence: 93%

Clinical Survey of Syphilis at the Dermatological Clinic of Nippon Medical School Hospital from 1984 to 1988, with Special Reference to the Recent Clinical Manifestations and Evaluation of IgM Antibodies to Treponema pallidum

Ishinaga

Sasaki

1990

The Journal of Dermatology

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One hundred eighty-one patients with syphilis were seen from May 1, 1984, to April 30, 1988 at the Dermatological Clinic of Nippon Medical School Hospital. The incidence of syphilis has increased gradually year by year. The number of early infectious syphilis cases was almost twice as high as late latent syphilis ones. As a source of infection, female prostitutes were noteworthy. Among primary syphilis cases, multiple chancres were observed in 29.2%. The frequency of ulcus durum was much higher than initial sclerosis. A relationship with oral sex is suggested. Among secondary syphilis cases, pruritus was observed in 23.9%, prominently on volar lesions. Psoriasiform papular and macular syphilide were the commonest features. Secondary syphilis with persisting chancres were seen in 41.3% and is gradually increasing. JH reactions were observed in 26.3%. The frequency was highest in late primary and in early secondary stages. IgM-TPHA and IgG-TPHA were tested in 94 sera by gel-filtration and 77 by HPLC. IgM-TPHA tests were reactive in virtually all the sera from untreated syphilis cases. The titres in untreated syphilis were higher than in treated cases. IgM-TPHA/IgM-TPHA + IgG-TPHA was higher in early syphilis than in late syphilis. Fifty-eight untreated cases were tested at frequent intervals after treatment for up to 12 months. IgM antibodies disappeared in 53 patients within 12 months. Non-treponemal antibodies measured by the CF test disappeared within 15 patients and TPHA tests remained positive after 12 months in all patients. IgM-TPHA may support a diagnosis of active syphilis.

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Latent Syphilis: Immunoglobulins Reactive in Immunofluorescence and Other Serological Tests

Cited by 14 publications

References 5 publications

Immunoglobulin Class of Natural Human Antibodies Reactive with Treponema pallidum

Immunoglobulin Class of Natural Human Antibodies Reactive with Treponema pallidum

Fluorescent treponemal antibody-absorption double-staining procedure

Clinical Survey of Syphilis at the Dermatological Clinic of Nippon Medical School Hospital from 1984 to 1988, with Special Reference to the Recent Clinical Manifestations and Evaluation of IgM Antibodies to Treponema pallidum

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