Latent Infection and the Elusive Cytomegalovirus

Jordan, M. Colin

doi:10.1093/clinids/5.2.205

Cited by 152 publications

(88 citation statements)

References 82 publications

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“…[19][20][21][22][23][24][25][26][27][28][29][30][31] In contrast, congenital (in utero) infection leads to infection of the central nervous system. In the immunocompromised host, CMV lesions may be found in numerous organs, including lung, brain, liver, gastrointestinal tract, and kidney.…”

Section: Discussionmentioning

confidence: 99%

Anatomical mapping of human herpesvirus reservoirs of infection

Chen

Hudnall

2006

Modern Pathology

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Section: Discussionmentioning

confidence: 99%

Anatomical mapping of human herpesvirus reservoirs of infection

Chen

Hudnall

2006

Modern Pathology

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“…Immunohistochemical detection methods might be expected to lack the sensitivity necessary to detect latent viral gene expression directly, although one recent report using immunohistochemical methods suggests that immediate early viral proteins are present in many cell types (Toorkey & Carrigan, 1989). In addition, it is not possible to detect HCMV sequences by dot blot hybridization analysis of leukocyte DNA from healthy seropositive subjects (Jordan, 1983). Consequently, more sensitive techniques are required to detect sites of HCMV persistence.…”

Section: Introductionmentioning

confidence: 99%

Monocytes are a major site of persistence of human cytomegalovirus in peripheral blood mononuclear cells

Taylor‐Wiedeman

Sissons

Borysiewicz

et al. 1991

Journal of General Virology

644

478

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We have used the nested polymerase chain reaction (PCR) combined with fluorescence-activated cell sorting to define sites of latency of human cytomegalovirus (HCMV) in the peripheral blood of healthy subjects. Peripheral blood mononuclear (PBM) cells were separated into T cell or non-T cell populations and monocytes, and were then analysed by PCR for the presence of HCMV DNA. In five of six seropositive subjects, HCMV was found predominantly in the non-T cell population. Further analysis suggested that the virus was present in adherent cells and CD 14 + cells. In three of nine seronegative subjects we could demonstrate HCMV DNA, which we do not believe was due to contamination, reproducibly by PCR. In one of these seronegative subjects, HCMV DNA was present predominantly in the non-T cell fraction of PBM cells. No HCMV DNA was detectable in the remaining six seronegative subjects. We conclude that, within the PBM cells of normal asymptomatic seropositive and some seronegative subjects, HCMV is present predominantly in the monocyte fraction. In addition, the detection of HCMV sequences in seronegative subjects may indicate that infection with HCMV is more widespread than conventional seroepidemiology suggests.

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“…Little is known about the nature of persistent infections with CMV, although there is evidence that the virus is harboured in various tissues such as kidney, liver, salivary glands and cervix and probably in cells of leukocyte lineage (Jordan, 1983). Virus is continuously excreted in the presence of circulating antibody (Reynolds et al, 1973) but cell-mediated immunity may be specifically impaired (Gehrz et al, 1977).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Cytomegalovirus DNA Is an Antiviral Target Late in Virus Growth

Tyms

Davis

Clarke

et al. 1987

Journal of General Virology

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SUMMARYThe mechanism of action of %(1,3-dihydroxypropoxymethyl)guanine (DHPG) and phosphonoformic acid (PFA) but not 5-fluorouridinedeoxyribose (FUdR), provides selective action against cytomegalovirus (CMV)-coded events and this was used to demonstrate that the synthesis of viral DNA was continuous during the extended phase of virus growth. The synthesis de novo of viral DNA was measured by restriction enzyme analysis after exposure to [32p]orthophosphate and its interruption by DHPG or PFA resulted in a cessation in the extrusion of infective virus from treated cells. The rate of decline in infectivity appeared to correspond to the failure of cells to maintain the synthesis of late proteins once DNA synthesis was blocked. Thus, regulation of late protein synthesis appeared to be linked to synthesis de novo of viral DNA even at late stages in CMV growth. The synthesis of the polyamines spermidine and spermine, considered obligatory for CMV growth, was unaffected by early or late inhibition of viral DNA and this showed that some virus-induced events were unaffected by the restriction on virus growth by DHPG. This provided evidence that polyamine biosynthesis was a target independent of viral DNA synthesis per se, which may be important in future considerations of combined drug therapies.

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Latent Infection and the Elusive Cytomegalovirus

Cited by 152 publications

References 82 publications

Anatomical mapping of human herpesvirus reservoirs of infection

Anatomical mapping of human herpesvirus reservoirs of infection

Monocytes are a major site of persistence of human cytomegalovirus in peripheral blood mononuclear cells

Synthesis of Cytomegalovirus DNA Is an Antiviral Target Late in Virus Growth

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