Late stage synchronization of respiratory syncytial virus replication

Levine, Seymour; Buthala, D. A.; Hamilton, Ramon D.

doi:10.1016/0042-6822(71)90340-0

Cited by 21 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After a latent period of 12--16 hours progeny virus is produced reaching a peak of 45--150 PFU/eell after 3648 hours post-infection but over 90 per cent of infectious virus remains cell associated (16,157,164,231,295). Optimal virus growth is dependent on an adequate supply of exogenous arginine (163,295). Optimal virus growth is dependent on an adequate supply of exogenous arginine (163,295).…”

Section: Replicationmentioning

confidence: 99%

Respiratory syncytial virus

Stott¹,

Taylor²

1985

Archives of Virology

211

126

View full text Add to dashboard Cite

Section: Replicationmentioning

confidence: 99%

Respiratory syncytial virus

Stott¹,

Taylor²

1985

Archives of Virology

211

126

View full text Add to dashboard Cite

“…Presumably only the latter are infectious. Thus, the consistently observed predominance of apparently incomplete virus might account for the generally low order of infectivity encountered with RS virus in any of several cell systems (10,12,13). Measurable infectivity may doubtless also be lost as a result of ultracentrifugation methods ordinarily used to concentrate RS virus from culture fluids.…”

Section: Fig 2 Same Asmentioning

confidence: 99%

Morphogenesis and Ultrastructure of Respiratory Syncytial Virus

Bächi

Howe

1973

J Virol

107

View full text Add to dashboard Cite

Respiratory syncytial (RS) virus was grown in Vero cells and fixed for electron microscopy at various stages of maturation. Both filamentous and round or kidney-shaped particles, either with (complete) or without (incomplete) internal structure, were observed. All four morphological forms were identical with respect to their reactivity with ferritin-labeled antibody to RS virus. Freezeetching revealed a structural feature apparently unique for RS virus, namely helical striations around the core on the internal aspect of the envelope. This specific configuration was already detectable during the early stages of viral differentiation of the host cell membrane. Concentration of free virus by zonal ultracentrifugation of culture fluids onto sucrose cushions yielded predominantly filamentous forms up to 10 um in length.

show abstract

“…The Long strain of RS virus was grown in HeLa cell monolayers maintained in MEM with 5% FBS, and assayed on HeLa monolayers by the plaque method previously described (Levine et al, 1971 ;Levine & Hamilton, 1969). Virus was labelled by growth in the presence of [3H]glucosamine (5 ~tCi/ml), [3H]mannose (5 ~tCi/ml), [3H]galactose (5 rtCi/ml), [3H]fucose (5 ~Ci/ml), [~C]glucosamine (2-5 ~tCi/ml) or [35S]methionine (10 btCi/ml), incorporated into MEM containing 5 % FBS.…”

Section: Methodsmentioning

confidence: 99%

Respiratory Syncytial Virus Polypeptides. IV. The Oligosaccharides of the Glycoproteins

Gruber

Levine

1985

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

SUMMARYThe cell-associated glycoproteins of respiratory syncytial (RS) virus included GP1 (90K), VP70 (70K), VGP48 (48K) and GP26 (26K). Although present in infected cells, there was no VP70 in purified virus. Trypsin treatment of infected cells removed 80 to 90% of VP70 as well as its products VGP48 and GP26. This suggested that most of the VP70 in the cell is located on the plasma membrane. The glycoproteins of purified RS virus (GP1, VGP48 and GP26) contain mannose, galactose and fucose as well as glucosamine, but the quantity of mannose in GP 1 is low when compared to that of the other three sugars. The effects that follow the treatment of infected cells with the glycosylation inhibitors tunicamycin and monensin, and the treatment of the immunoprecipitated product of pulse-chase experiments with endonuclease H demonstrated that VP70 and its products contained N-linked oligosaccharides, and that the oligosaccharides of the mature VGP48 subunit were of the complex type, while GP1 contained both N-and O-linked oligosaccharides. The non-glycosylated forms of VP70 and GP1 have estimated mol. wt. of 50K and 33K respectively. Therefore, the carbohydrate contribution to the mol. wt. of VP70 and GP1, as determined by PAGE, was equivalent to 20K for the former and 57K for the latter. The majority of the GP1 oligosaccharides were O-linked, a form of sugar linkage not previously found among paramyxoviruses.

show abstract

Late stage synchronization of respiratory syncytial virus replication

Cited by 21 publications

References 20 publications

Respiratory syncytial virus

Respiratory syncytial virus

Morphogenesis and Ultrastructure of Respiratory Syncytial Virus

Respiratory Syncytial Virus Polypeptides. IV. The Oligosaccharides of the Glycoproteins

Contact Info

Product

Resources

About