1971
DOI: 10.1016/0042-6822(71)90340-0
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Late stage synchronization of respiratory syncytial virus replication

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Cited by 21 publications
(9 citation statements)
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“…After a latent period of 12--16 hours progeny virus is produced reaching a peak of 45--150 PFU/eell after 3648 hours post-infection but over 90 per cent of infectious virus remains cell associated (16,157,164,231,295). Optimal virus growth is dependent on an adequate supply of exogenous arginine (163,295). Optimal virus growth is dependent on an adequate supply of exogenous arginine (163,295).…”
Section: Replicationmentioning
confidence: 99%
“…After a latent period of 12--16 hours progeny virus is produced reaching a peak of 45--150 PFU/eell after 3648 hours post-infection but over 90 per cent of infectious virus remains cell associated (16,157,164,231,295). Optimal virus growth is dependent on an adequate supply of exogenous arginine (163,295). Optimal virus growth is dependent on an adequate supply of exogenous arginine (163,295).…”
Section: Replicationmentioning
confidence: 99%
“…Presumably only the latter are infectious. Thus, the consistently observed predominance of apparently incomplete virus might account for the generally low order of infectivity encountered with RS virus in any of several cell systems (10,12,13). Measurable infectivity may doubtless also be lost as a result of ultracentrifugation methods ordinarily used to concentrate RS virus from culture fluids.…”
Section: Fig 2 Same Asmentioning
confidence: 99%
“…The Long strain of RS virus was grown in HeLa cell monolayers maintained in MEM with 5% FBS, and assayed on HeLa monolayers by the plaque method previously described (Levine et al, 1971 ;Levine & Hamilton, 1969). Virus was labelled by growth in the presence of [3H]glucosamine (5 ~tCi/ml), [3H]mannose (5 ~tCi/ml), [3H]galactose (5 rtCi/ml), [3H]fucose (5 ~Ci/ml), [~C]glucosamine (2-5 ~tCi/ml) or [35S]methionine (10 btCi/ml), incorporated into MEM containing 5 % FBS.…”
Section: Methodsmentioning
confidence: 99%