Late Miocene diversification and phylogenetic relationships of the huge toads in the Rhinella marina (Linnaeus, 1758) species group (Anura: Bufonidae)

Maciel, Natan Medeiros; Collevatti, Rosane Garcia; Colli, Guarino R.; Schwartz, Elisabeth Ferroni

doi:10.1016/j.ympev.2010.08.025

Cited by 70 publications

(69 citation statements)

References 51 publications

(93 reference statements)

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“…Pramuk 2006). As a definite outgroup we employed a sample of Rhinella poeppigii (Tschudi, 1845) from a distant species group of the Rhinella marina species group (Pramuk 2006; Maciel et al 2010). Our dataset was further supplemented by sequences from GenBank to include representatives of all earlier published genetic lineages of the Rhinella margaritifera species group (Pramuk 2006; Fouquet et al 2007a, 2012a, b; Jansen et al 2011).…”

Section: Methodsmentioning

confidence: 99%

A new species of the Rhinella margaritifera species group (Anura, Bufonidae) from the montane forest of the Selva Central, Peru

Moravec¹,

Lehr²,

Cusi³

et al. 2014

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We describe a new species of the bufonid toad genus Rhinella from transition montane forest of the buffer zones of the Yanachaga-Chemillén National Park and the Pui Pui Protected Forest (eastern slopes of Andes, Selva Central, Peru). The new species belongs to the Rhinella margaritifera species group (confirmed by mtDNA data) and differs from all its members by the absence of tympanic membrane and tympanic annulus. It is characterized by medium size (SVL 57.5–65.5 mm, n = 5), moderately developed cranial crests, absence of neural crest of vertebrae, absence of bone protrusion at angle of jaw, presence of lateral rows of enlarged tubercles, and absence of subgular vocal sac and vocal slits in males. In addition, based on the molecular phylogenetic analyses of selected Rhinella species we propose the monophylum containing R. chavin, R. festae, R. macrorhina, R. manu, R. nesiotes, R. rostrata, and R. yanachaga as a new species group under the name Rhinella festae species group.

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Section: Methodsmentioning

confidence: 99%

A new species of the Rhinella margaritifera species group (Anura, Bufonidae) from the montane forest of the Selva Central, Peru

Moravec¹,

Lehr²,

Cusi³

et al. 2014

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“…For this purpose, we defined three groups: i) R. marina sequences were divided in the two different geographic groups of populations, RAB and LAB; and, ii) all R . schneideri sequences were defined as a group, The toads R. icterica and R. arenarum were used as outgroups [24,25]. The input file for *BEAST was created using the application BEAUti [82] and partitions and models were edited by hand to fit models for each unlinked nuclear fragment, as determined with Kakusan4.…”

Section: Methodsmentioning

confidence: 99%

“…These species are morphologically similar (but diagnosable by the presence of tibial glands in R. schneideri ) and have essentially a parapatric distribution, but may occur in sympatry along ecological transitions between humid and dry forests (Figure 1). Previous phylogenetic analyses of the R. marina group based on mtDNA [24,25] suggested a division in two major clades: one including R. veredas , R. cerradensis , R. jimi , R. marina , R . schneideri , and R. poeppigii (north-central clade), and another including R. arenarum , R. rubescens , R. achavali , and R. icterica (south-central clade).…”

Section: Introductionmentioning

confidence: 99%

Hybridization and massive mtDNA unidirectional introgression between the closely related Neotropical toads Rhinella marina and R. schneideriinferred from mtDNA and nuclear markers

et al. 2011

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BackgroundThe classical perspective that interspecific hybridization in animals is rare has been changing due to a growing list of empirical examples showing the occurrence of gene flow between closely related species. Using sequence data from cyt b mitochondrial gene and three intron nuclear genes (RPL9, c-myc, and RPL3) we investigated patterns of nucleotide polymorphism and divergence between two closely related toad species R. marina and R. schneideri. By comparing levels of differentiation at nuclear and mtDNA levels we were able to describe patterns of introgression and infer the history of hybridization between these species.ResultsAll nuclear loci are essentially concordant in revealing two well differentiated groups of haplotypes, corresponding to the morphologically-defined species R. marina and R. schneideri. Mitochondrial DNA analysis also revealed two well-differentiated groups of haplotypes but, in stark contrast with the nuclear genealogies, all R. schneideri sequences are clustered with sequences of R. marina from the right Amazon bank (RAB), while R. marina sequences from the left Amazon bank (LAB) are monophyletic. An Isolation-with-Migration (IM) analysis using nuclear data showed that R. marina and R. schneideri diverged at ≈ 1.69 Myr (early Pleistocene), while R. marina populations from LAB and RAB diverged at ≈ 0.33 Myr (middle Pleistocene). This time of divergence is not consistent with the split between LAB and RAB populations obtained with mtDNA data (≈ 1.59 Myr), which is notably similar to the estimate obtained with nuclear genes between R. marina and R. schneideri. Coalescent simulations of mtDNA phylogeny under the speciation history inferred from nuclear genes rejected the hypothesis of incomplete lineage sorting to explain the conflicting signal between mtDNA and nuclear-based phylogenies.ConclusionsThe cytonuclear discordance seems to reflect the occurrence of interspecific hybridization between these two closely related toad species. Overall, our results suggest a phenomenon of extensive mtDNA unidirectional introgression from the previously occurring R. schneideri into the invading R. marina. We hypothesize that climatic-induced range shifts during the Pleistocene/Holocene may have played an important role in the observed patterns of introgression.

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“…The studied species belong to the R. marina group and are phylogenetically close to each other. The group contains 10 species distributed throughout different habitats from Texas to Uruguay (11). R. jimi (Stevaux, 2002) are originally from xeric environments (Brazilian Caatinga region) and face temperatures from 20°C to more than 40°C.…”

Section: Introductionmentioning

confidence: 99%

Analysis of heart rate control to assess thermal sensitivity responses in Brazilian toads

Natali¹,

Santos²,

Rodrigues³

et al. 2015

Braz J Med Biol Res

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In anurans, changes in ambient temperature influence body temperature and, therefore, energy consumption. These changes ultimately affect energy supply and, consequently, heart rate (HR). Typically, anurans living in different thermal environments have different thermal sensitivities, and these cannot be distinguished by changes in HR. We hypothesized that Rhinella jimi (a toad from a xeric environment that lives in a wide range of temperatures) would have a lower thermal sensitivity regarding cardiac control than R. icterica (originally from a tropical forest environment with a more restricted range of ambient temperatures). Thermal sensitivity was assessed by comparing animals housed at 15° and 25°C. Cardiac control was estimated by heart rate variability (HRV) and heart rate complexity (HRC). Differences in HRV between the two temperatures were not significant (P=0.214 for R. icterica and P=0.328 for R. jimi), whereas HRC differences were. All specimens but one R. jimi had a lower HRC at 15°C (all P<0.01). These results indicate that R. jimi has a lower thermal sensitivity and that cardiac control is not completely dependent on the thermal environment because HRC was not consistently different between temperatures in all R. jimi specimens. This result indicates a lack of evolutive trade-offs among temperatures given that heart rate control at 25°C is potentially not a constraint to heart rate control at 15°C.

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Late Miocene diversification and phylogenetic relationships of the huge toads in the Rhinella marina (Linnaeus, 1758) species group (Anura: Bufonidae)

Cited by 70 publications

References 51 publications

A new species of the Rhinella margaritifera species group (Anura, Bufonidae) from the montane forest of the Selva Central, Peru

A new species of the Rhinella margaritifera species group (Anura, Bufonidae) from the montane forest of the Selva Central, Peru

Hybridization and massive mtDNA unidirectional introgression between the closely related Neotropical toads Rhinella marina and R. schneideriinferred from mtDNA and nuclear markers

Analysis of heart rate control to assess thermal sensitivity responses in Brazilian toads

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