Late Embryogenesis Abundant (LEA) protein gene regulation during Arabidopsis seed maturation

Delseny, Michel; Bies‐Etheve, Natacha; Carles, Christel; Hull, Gillian; Vicient, Carlos M.; Raynal, Monique; Grellet, Françoise; Aspart, Lorette

doi:10.1078/0176-1617-00353

Cited by 47 publications

(55 citation statements)

References 42 publications

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“…ABI5 is expressed in a variety of distinct cell types through development, showing some overlap with ABF3 and ABF4 expression in vegetative and floral tissues (Kang et al, 2002), and it is likely that its physiological role varies among tissues and stages. The highest ABI5 expression is observed in mature dry seeds, where it has been shown to regulate some late embryogenesis abundant genes (Finkelstein and Lynch, 2000;Lopez-Molina and Chua, 2000;Delseny et al, 2001), at least some of which are correlated with desiccation tolerance (Swire-Clark and Marcotte, 1999). A similar role might be played in anthers, where maturing pollen must also acquire desiccation tolerance.…”

Section: Discussionmentioning

confidence: 99%

Regulation and Role of the ArabidopsisAbscisic Acid-Insensitive 5Gene in Abscisic Acid, Sugar, and Stress Response

2002

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Abscisic acid (ABA) and stress response from late embryonic growth through early seedling development is regulated by a signaling network that includes the Arabidopsis ABA-insensitive (ABI)5 gene, which encodes a basic leucine zipper transcription factor. We have characterized genetic, developmental, and environmental regulation of ABI5 expression. Although expressed most strongly in seeds, the ABI5 promoter is also active in vegetative and floral tissue. Vegetative expression is strongly induced by ABA, and weakly by stress treatments during a limited developmental window up to approximately 2 d post-stratification, but ABA and some stresses can induce expression in specific tissues at later stages. ABI5 expression is autoregulated in transgenic plants and yeast (Saccharomyces cerevisiae), and stress response appears to involve ABI5-dependent and -independent mechanisms. To determine whether ABI5 is necessary and/or sufficient for ABA or stress response, we assayed the effects of increased ABI5 expression on growth and gene expression. Although overexpression of ABI5 confers hypersensitivity to ABA and sugar, as previously described for ABI4 and ABI3 overexpression lines, it has relatively limited effects on enhancing ABA-responsive gene expression. Comparison of expression of eight ABI5-homologous genes shows overlapping regulation by ABI3, ABI4, and ABI5, suggestive of a combinatorial network involving positive and negative regulatory interactions.The phytohormone abscisic acid (ABA) regulates many agronomically important aspects of plant growth and development, including seed maturation, dormancy, stress tolerances, and water relations (for review, see Leung and Giraudat, 1998; Rock, 2000;. All of these processes are regulated by additional signals, including other phytohormones, stage-specific regulators, and abiotic stresses. Studies of ABA-deficient mutants have shown that ABA is an essential mediator in triggering some plant responses to abiotic stresses, including drought, salinity, and cold (Rock, 2000; Shinozaki and Yamaguchi-Shinozaki, 2000; Xiong and Zhu, 2001). Dehydration and low temperatures result in elevated levels of ABA, which, in turn, trigger the synthesis of some proteins responsible for drought or freezing tolerance. However, although most of the drought-and salt-induced genes studied to date can be induced by ABA, many aspects of stress response are also mediated by ABA-independent mechanisms (Shinozaki and Yamaguchi-Shinozaki, 2000). These results indicate that ABA participates in only part of a network of stress-signaling mechanisms. Furthermore, even the ABA-dependent portions of this network rely on both independent and partially redundant signaling components, some of which are produced only in specific developmental stages or tissues of the plant.Components of this signaling network have been identified by a combination of biochemical, cell biological, and forward and reverse genetic approaches. To date, nearly 50 loci in Arabidopsis alone have been demonstrated to function in v...

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Section: Discussionmentioning

confidence: 99%

Regulation and Role of the ArabidopsisAbscisic Acid-Insensitive 5Gene in Abscisic Acid, Sugar, and Stress Response

2002

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“…In control seeds in which germination was inhibited, mRNA abundance of a number of genes either declined somewhat or was constant following imbibition, but remained elevated after 48 h at 35°C. Among these were Ls-ZEP1 ( Figure 3B), a homolog of the Arabidopsis ABA1 gene encoding an ABA biosynthetic enzyme (Seo and Koshiba, 2002); Ls-ABA8ox4 ( Figure 3C), a homolog of the Arabidopsis CYP707A2 gene encoding an ABA 8'-hydroxlase that initiates ABA catabolism Sawada et al, 2008); transcription factors involved in the ABA signal transduction pathway regulating germination (e.g., Ls-ABI5 and Ls-ABI3; Figures 3D and 3E) (Finkelstein et al, 2008); Ls-SNF4 ( Figure 3F), an ABA-induced gene encoding a regulatory subunit of the SnRK1 kinase family involved in metabolic regulation in seeds (Bradford et al, 2003;Radchuk et al, 2006;Rosnoblet et al, 2007); Ls-GA2ox1 ( Figure 3H), encoding a GA catabolic enzyme (Toh et al, 2008); Ls-LEA ( Figure 3L), encoding a late embryogenesis abundant protein (Delseny et al, 2001); SPATULA ( Figure 3M), a temperature-dependent germination repressor (Penfield et al, 2005); and Ls-DOG1 ( Figure 3O), a homolog of Arabidopsis DOG1, which is involved in the induction and maintenance of seed dormancy (Bentsink et al, 2006; Footitt et al, 2011;Kendall et al, 2011). All of these genes exhibited a tendency for a more rapid decrease in transcript abundance in the RNAi seeds following imbibition, and their mRNAs were very low or absent by 48 h when germination had been completed.…”

Section: Gene Expression In Nced4-rnai Lettuce Seeds Imbibed At High mentioning

confidence: 99%

Expression of9-cis-EPOXYCAROTENOID DIOXYGENASE4Is Essential for Thermoinhibition of Lettuce Seed Germination but Not for Seed Development or Stress Tolerance

et al. 2013

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Thermoinhibition, or failure of seeds to germinate at warm temperatures, is common in lettuce (Lactuca sativa) cultivars. Using a recombinant inbred line population developed from a lettuce cultivar (Salinas) and thermotolerant Lactuca serriola accession UC96US23 (UC), we previously mapped a quantitative trait locus associated with thermoinhibition of germination to a genomic region containing a gene encoding a key regulated enzyme in abscisic acid (ABA) biosynthesis, 9-cis-EPOXYCAROTENOID DIOXYGENASE4 (NCED4). NCED4 from either Salinas or UC complements seeds of the Arabidopsis thaliana nced6-1 nced9-1 double mutant by restoring germination thermosensitivity, indicating that both NCED4 genes encode functional proteins. Transgenic expression of Salinas NCED4 in UC seeds resulted in thermoinhibition, whereas silencing of NCED4 in Salinas seeds led to loss of thermoinhibition. Mutations in NCED4 also alleviated thermoinhibition. NCED4 expression was elevated during late seed development but was not required for seed maturation. Heat but not water stress elevated NCED4 expression in leaves, while NCED2 and NCED3 exhibited the opposite responses. Silencing of NCED4 altered the expression of genes involved in ABA, gibberellin, and ethylene biosynthesis and signaling pathways. Together, these data demonstrate that NCED4 expression is required for thermoinhibition of lettuce seeds and that it may play additional roles in plant responses to elevated temperature.

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“…The ABI5 family of proteins all share three conserved charged domains (present in the N-terminal half of the protein and containing potential phosphorylation sites) and a bZIP domain at the C-terminus. Like VP1/ABI3, ABI5 can either activate or repress gene expression, and ABI3 and ABI5 may have antagonistic or synergistic effects on gene expression, depending on the gene Delseny and others 2001).…”

Section: Aba Signaling Pathway Components: Putative Roles In Developmmentioning

confidence: 99%

Role of Abscisic Acid in Seed Dormancy

Kermode

2005

J Plant Growth Regul

242

156

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Seed dormancy is an adaptive trait that improves survival of the next generation by optimizing the distribution of germination over time. The agricultural and forest industries rely on seeds that exhibit high rates of germination and vigorous, synchronous growth after germination; hence dormancy is sometimes considered an undesirable trait. The forest industry encounters problems with the pronounced dormancy of some conifer seeds, a feature that can lead to non-uniform germination and poor seedling vigor. In cereal crops, an optimum balance is most sought after; some dormancy at harvest is favored because it prevents germination of the physiologically mature grain in the head prior to harvest (that is, preharvest sprouting), a phenomenon that leads to considerable damage to grain quality and is especially prominent in cool moist environments. The sesquiterpene abscisic acid (ABA) regulates key events during seed formation, such as the deposition of storage reserves, prevention of precocious germination, acquisition of desiccation tolerance, and induction of primary dormancy. Its regulatory role is achieved in part by cross-talk with other hormones and their associated signaling networks, via mechanisms that are largely unknown. Quantitative genetics and functional genomics approaches will contribute to the elucidation of genes and proteins that control seed dormancy and germination, including components of the ABA signal transduction pathway. Dynamic changes in ABA biosynthesis and catabolism elicit hormone-signaling changes that affect downstream gene expression and thereby regulate critical checkpoints at the transitions from dormancy to germination and from germination to growth. Some of the recent developments in these areas are discussed.

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Late Embryogenesis Abundant (LEA) protein gene regulation during Arabidopsis seed maturation

Cited by 47 publications

References 42 publications

Regulation and Role of the ArabidopsisAbscisic Acid-Insensitive 5Gene in Abscisic Acid, Sugar, and Stress Response

Regulation and Role of the ArabidopsisAbscisic Acid-Insensitive 5Gene in Abscisic Acid, Sugar, and Stress Response

Expression of9-cis-EPOXYCAROTENOID DIOXYGENASE4Is Essential for Thermoinhibition of Lettuce Seed Germination but Not for Seed Development or Stress Tolerance

Role of Abscisic Acid in Seed Dormancy

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