Late Biliary Obstruction in Wistar Rats After Intermittent Hepatic Pedicle Clamping

Jorge, Gracinda de Lourdes; Tártaro, Rodolfo dos Reis; Facin, Andréia Coutinho; Pereira, Renato Augusto; Escanhoela, C.A.F.; Boin, I.F.S.F.

doi:10.1016/j.transproceed.2014.05.067

Cited by 5 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, the new technique for temporary BDO using a vascular clip is a safe, fast, and inexpensive method without significant mortality. Similar observations concerning safety and usage of a vascular clip device were published by Jorge with experimental intermittent hepatic pedicle clamping and temporary choledochal clamping (~10 min) in rats [7], [12], [13].…”

Section: Discussionsupporting

confidence: 62%

“…The second endpoint in our study was the suitability of the clip to induce comparable systemic and histological alterations, as already described with the “ligation technique of BDL” [7], [8], [12], [13], [14], [15]. BDO led to a strong increase in systemic cholestatic parameters, while after removal of the clip we determined a rapid decline of these parameters [7], [8], [12], [13], [14], [15]. Interestingly, in our setting, the common bile duct showed no remaining dilatation after the removal of the vascular clip.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, in our setting, the common bile duct showed no remaining dilatation after the removal of the vascular clip. In contrast, Jorge described in his setting (his clip was removed after 10 min) a residual dilatation of the main bile duct for up to 28 days [12], [13]. As Jorge described no further details of the used vascular clip, it led to the suspicion that the closing force is a crucial factor for visible and remaining dilatation of the main bile duct.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Reversible biliary occlusion in a small animal model: first description of a new technique

Richter

Khodaverdi

Bechstein

et al. 2018

Innovative Surgical Sciences

View full text Add to dashboard Cite

BackgroundExperimental models with reversible biliary occlusion resulted in a high mortality of the animals, up to 20–60% according to the literature. Our aim was to assess a safe and valid technique for reversible biliary occlusion with a low mortality.MethodsWe randomized 30 rats into two groups: with bile duct occlusion (BDO, n=18) and with sham manipulation of the extrahepatic bile duct (control, n=12). We used a removable vascular clip for temporary occlusion of the extrahepatic bile duct. The clip was removed on postoperative day (POD) 2. On POD 2, 3, and 5, we measured the hepatocellular injury and metabolic function markers in serum. Activation of mononuclear cells (HIS36) and expression of regeneration markers [cytokeratin 19, hepatic growth factor (HGF)-α, and HGF-β] were determined by immunohistochemistry.ResultsThe survival rate was 96.67% (1/30); one animal died. The mortality in the BDO group was 6% (1/18) and that in the control group was 0% (0/12). BDO resulted in a sharp increase of hepatocellular injury and cholestatic parameters on POD 2 with a rapid decline till POD 3. Significantly strongest activation of Kupffer cells and expression of proliferation markers were found until POD 5 after BDO.ConclusionThe clip technique is a safe, cheap, and valid method for reversible biliary occlusion with an extremely low mortality.

show abstract

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Reversible biliary occlusion in a small animal model: first description of a new technique

Richter

Khodaverdi

Bechstein

et al. 2018

Innovative Surgical Sciences

View full text Add to dashboard Cite

show abstract

“…After the anesthetic procedure, trichotomy incision in a U-shape [7] was carried out. Isolation of the hepatic pedicle (hepatic artery, portal vein, and bile duct) with flexible cotton buds was performed in each group before intervention.…”

Section: Methodsmentioning

confidence: 99%

“…It is important to evaluate hepatic rat tissue through an intermittent long-time ischemia period such as hepatic protectors. Some research has shown that severe liver injury is identified in the postoperative period even in short-time clamping of the hepatic pedicle in rats [7].…”

mentioning

confidence: 99%

No Protective Function Found in Wistar Rats Submitted to Long Ischemia Time and Reperfusion After Intermittent Clamping of the Total Hepatic Pedicle

Tártaro

Jorge

Leonardi

et al. 2015

Transplantation Proceedings

Self Cite

View full text Add to dashboard Cite

show abstract

Long-Time Choledochal Clamping in Wistar Rats Causes Biliary Obstruction Progressing to Hepatic Fibrosis

Jorge

Tártaro

Escanhoela

et al. 2016

Transplantation Proceedings

View full text Add to dashboard Cite

show abstract

Late Biliary Obstruction in Wistar Rats After Intermittent Hepatic Pedicle Clamping

Cited by 5 publications

References 20 publications

Reversible biliary occlusion in a small animal model: first description of a new technique

Reversible biliary occlusion in a small animal model: first description of a new technique

No Protective Function Found in Wistar Rats Submitted to Long Ischemia Time and Reperfusion After Intermittent Clamping of the Total Hepatic Pedicle

Long-Time Choledochal Clamping in Wistar Rats Causes Biliary Obstruction Progressing to Hepatic Fibrosis

Contact Info

Product

Resources

About