Lasers for gamete micromanipulation: Basic concepts

Tadir, Yona; Neev, Joseph; Ho, Peter D.; Berns, Michael W.

doi:10.1007/bf01207733

Cited by 28 publications

(18 citation statements)

References 13 publications

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“…Application of ultraviolet (UV) lasers in non-contact mode has been proposed by Tadir et al (1993) for different micromanipulation procedures. These systems have the advantage that there is no need for disposable fibres and for sterilization; also, there is the possibility of intracellular application.…”

Section: Discussionmentioning

confidence: 99%

Fertilization and early embryology: Use of lasers in assisted fertilization and hatching

Obruca¹,

Strohmer²,

Sakkas

et al. 1994

Human Reproduction

102

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The erbium-yttrium-aluminium-garnet (Er:YAG) laser has been applied to micromanipulation in humans. It was used in the fertilization process for both subzonal insemination (SUZI) and for partial zona dissection (PZD). Laser-assisted micromanipulation achieved significantly higher fertilization rates (34.8%) when compared to mechanical SUZI (16.1%), but use of the laser did not improve the PZD results (laser 14.8% versus mechanical 14%). The Er:YAG laser was used to assist hatching. In the mouse it significantly improved the hatching rate (80 versus 29.3%) 110 h after administration of human chorionic gonadotrophin. This technique was applied in two different centres to patients with previous invitro fertilization (IVF) failures. The implantation rate per embryo (14.4% laser-assisted hatching versus 6% control group) and the pregnancy rate per transfer (40 versus 16.2%) were improved.

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Section: Discussionmentioning

confidence: 99%

Fertilization and early embryology: Use of lasers in assisted fertilization and hatching

Obruca¹,

Strohmer²,

Sakkas

et al. 1994

Human Reproduction

102

View full text Add to dashboard Cite

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“…In previous studies (1,2,5,12) we demonstrated the great utility and precision of the laser (at the appropriate parameters) as a superior device for micromanipulation and precise breaching of the ZP. In Fig.…”

Section: Discussionmentioning

confidence: 98%

Assisted hatching in mouse embryos using a noncontact Ho:YSGG laser system

Neev

Schiewe

Sung

et al. 1995

J Assist Reprod Genet

Self Cite

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“…Other methods of hatching have been described, from mechanical methods 7 to the use of a laser 8 . In spite of this widespread use, there is still debate as to its efficacy, with many studies published producing contrasting results, some firmly against any benefit 9,10 while others are strongly supportive of its benefits 11,12 .…”

Section: Discussionmentioning

confidence: 99%

Partial hydatidiform mole following intracytoplasmic sperm injection and assisted zona hatching

Wood

Sephton

Searle

et al. 2002

BJOG

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Case reportA 35 year old women underwent her second cycle of in vitro fertilisation (IVF). She had an eight-year history of primary infertility due to endometriosis and male subfertility. The couple had undergone a cycle of gamete intrafallopian transfer six years previously, at which time only one oocyte was retrieved. The following year, she underwent a cycle of IVF when three oocytes were recovered, of which one fertilised and was replaced, without success. Her baseline investigations showed an follicle-stimulating hormone level of 5.7 u/L, luteinising hormone of 3.0 u/L and prolactin of 306 iu/L; her blood group was B Rhþve. The semen analysis showed a concentration of 80 Â 10 6 /mL, 11% normal forms (WHO criteria) and 17% progressive motility. Following these results, it was decided that intracytoplasmic sperm injection (ICSI) was the most appropriate procedure. Her partner's karyotype was 46 XY, with no gross abnormalities.She was down-regulated with subcutaneous buserelin (Shire Pharmaceuticals, Hants, UK) using a standard longprotocol regimen from the midluteal phase of her previous menstrual cycle. After confirmation of satisfactory down-regulation by a transvaginal ultrasound scan, she underwent ovarian stimulation with 300 iu of human menopausal gonadotrophin (hMG, Menogon, Ferring, Kiel, Germany). After five days, a repeat ultrasound scan showed poor follicular development. The dose of hMG was therefore increased to 450 iu daily for a further seven days, at which time a repeat ultrasound scan showed four follicles between 18 and 25 mm in diameter. That evening, 5000 iu of human chorionic gonadotrophin (hCG) were given intramuscularly, and 36 hours later four oocytes were recovered transvaginally.Four hours after collection, the oocytes were denuded of their cumulus and coronal cells immediately prior to ICSI being performed. All four oocytes were assessed as being at metaphase II and were thus injected. The semen analysis on that day showed a volume of 5 mL, a concentration of 60 Â 10 6 /mL, 32% normal morphology and 40% progressive motility. The sample was prepared using a conventional swim-up technique.Eighteen hours following the ICSI procedure, the four oocytes were examined for evidence of fertilisation. One oocyte contained two clear pronuclei and was assumed to have fertilised 'normally'. One oocyte contained only one pronucleus, although two polar bodies were observed. The remaining two oocytes showed no evidence of fertilisation. These three oocytes were allowed to perish. After a further 24 hours, the normally fertilised oocyte had undergone two cleavage divisions to reach the four-cell stage. No evidence of blastomere fragmentation or irregularity was observed. Before transfer to the woman, this embryo was subjected to assisted zona hatching using acid tyrose solution, as first described by Cohen et al.1 . Luteal support was given in the form of progesterone pessaries (Cyclogest, Shire Pharmaceuticals) begun the day before embryo transfer. Thirteen days after transfer, the woman reported m...

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Lasers for gamete micromanipulation: Basic concepts

Cited by 28 publications

References 13 publications

Fertilization and early embryology: Use of lasers in assisted fertilization and hatching

Fertilization and early embryology: Use of lasers in assisted fertilization and hatching

Assisted hatching in mouse embryos using a noncontact Ho:YSGG laser system

Partial hydatidiform mole following intracytoplasmic sperm injection and assisted zona hatching

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